Antioxidants in aqueous extract of Myristica fragrans (Houtt.) suppress mitosis and cyclophosphamide-induced chromosomal aberrations in Allium cepa L. cells

In this study, freeze-dried water extract from the leaves of Myristica fragrans (Houtt.) was tested for mutagenic and antimutagenic potentials using the Allium cepa assay. Freeze-dried water extract alone and its combination with cyclophosphamide (CP) (50 mg/kg) were separately dissolved in tap water at 500, 1000, 2000, and 4000 mg/kg. Onions (A. cepa) were suspended in the solutions and controls for 48 h in the dark. Root tips were prepared for microscopic evaluation. 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radicals’ scavenging power of the extract was tested using butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) as standards. Water extract of Myristica fragrans scavenged free radicals better than BHA, but worse than BHT. The extract alone, as well as in combination with CP suppressed cell division, and induced chromosomal aberrations that were insignificantly different from the negative control (P≤0.05). However, cytotoxic and mutagenic actions of CP were considerably suppressed. The observed effects on cell division and chromosomes of A. cepa may be principally connected to the antioxidant properties of the extract. The obtained results suggest mitodepressive and antimutagenic potentials of water extract of the leaves of M. fragrans as desirable properties of a promising anticancer agent.     

Teacher change beliefs: validating a scale with structural equation modelling

The objectives of the study were to validate a substantiated Teacher Change Beliefs Model (TCBM) and an instrument to identify critical components of teacher change beliefs (TCB) in Malaysian secondary schools. Five different pilot test approaches were applied to ensure the validity and reliability of the instrument. A total of 936 teachers from 47 high-performing secondary schools completed the survey. Structural equation modelling was applied to test the models. Exploratory factor analysis was employed to identify the underlying factors, whereas confirmatory factor analysis was applied to test the measurement models. The analysis yielded a three-factor TCBM: (1) discrepancy, (2) efficacy and (3) principal support. The results demonstrated a good fit of the model: normed χ² = 3.156, Tucker-Lewis Fix Index = .987, Comparative Fix Index = .991 and Root Mean Square Error of Approximation = .048. The results also provided evidence for convergent validity, discriminant validity and construct reliability. The TCBM is an empirically tested model derived in a local Malaysian cultural education setting. It provides direction for practitioners in planning and designing training programmes of change management for school principals in the enhancement of TCB among teachers in schools. Besides, Teacher Change Beliefs Scale is a promising and welcome tool for both practitioners and researchers. With only nine items, it is easy to administer and not time-consuming.     

Reconceptualizing student ratings of teaching to support quality discourse on student learning: a systems perspective

Higher Education - This paper summarizes the discourse on student ratings of teaching in higher education. It reconceptualizes student ratings within a larger process of promoting quality in...     

Robust stabilization of nonlinear time delay systems: A complete type functionals approach

The robust stabilization of some classes of nonlinear delay systems with nominal linear delay system is addressed. The form of the controller is not an a priori proposal, but it is the result of a synthesis relying on the use of complete type Lyapunov–Krasovskii functionals, leading to distributed delay linear or nonlinear robust control laws. Simulation results of the stabilization of a chemical refining process demonstrate the good performance of the proposed approaches.     

Synthesis of singly-terminated ladder networks by multivariable array using homogeneous polynomials

This paper discusses a multivariable array whose elements are multivariable homogeneous polynomials which may have missing terms in any of the variables. This array permits one to obtain conditions for the realizability of a driving-point function or a transfer function as a resistively terminated low-pass ladder network. By reac- tance transformations, other forms of ladder structures can be obtained. The case of cascade-separable ladder networks is discussed in detail.     

Study of resonance-cone propagation in truncated hyperbolic metamaterial grids using transmission-line matrix simulations

In this paper, we utilize transmission-line matrix simulations to study the electromagnetic properties of hyperbolic metamaterial grids, which are synthesized by arranging unloaded transmission lines over electrical ground planes, such that the unit cells are rectangular in shape. The hyperbolic grids support Bragg and Hyperbolic resonant modes that have rectangular and hyperbolic dispersion surfaces, respectively. We demonstrate the formation of these resonant modes in the hyperbolic grids and their refraction, and spatial scanning properties via circuit simulations. The simulation results are compared with experimental results of a two-layer anisotropic hyperbolic metamaterial.     

A CRISPR/Cas12a-empowered surface plasmon resonance platform for rapid and specific diagnosis of the Omicron variant of SARS-CoV-2

The outbreak of the COVID-19 pandemic was partially due to the challenge of identifying asymptomatic and presymptomatic carriers of the virus, and thus highlights a strong motivation for diagnostics with high sensitivity that can be rapidly deployed. On the other hand, several concerning SARS-CoV-2 variants, including Omicron, are required to be identified as soon as the samples are identified as ‘positive’. Unfortunately, a traditional PCR test does not allow their specific identification. Herein, for the first time, we have developed MOPCS (Methodologies of Photonic CRISPR Sensing), which combines an optical sensing technology-surface plasmon resonance (SPR) with the ‘gene scissors’ clustered regularly interspaced short palindromic repeat (CRISPR) technique to achieve both high sensitivity and specificity when it comes to measurement of viral variants. MOPCS is a low-cost, CRISPR/Cas12a-system-empowered SPR gene-detecting platform that can analyze viral RNA, without the need for amplification, within 38 min from sample input to results output, and achieve a limit of detection of 15 fM. MOPCS achieves a highly sensitive analysis of SARS-CoV-2, and mutations appear in variants B.1.617.2 (Delta), B.1.1.529 (Omicron) and BA.1 (a subtype of Omicron). This platform was also used to analyze some recently collected patient samples from a local outbreak in China, identified by the Centers for Disease Control and Prevention. This innovative CRISPR-empowered SPR platform will further contribute to the fast, sensitive and accurate detection of target nucleic acid sequences with single-base mutations.     

Tide–surge interaction model including air bubble effects for the coast of Bangladesh

An estimate of water levels due to the interaction of tide and surge associated with a storm including air bubble effects is made along the coastal region of Bangladesh. For this purpose, a vertically integrated shallow water model in Cartesian coordinate system is developed introducing air bubbles and is solved using nested finite difference schemes. A fine grid model covering the region between 21°15′N to 23°N and 89°E to 92° E has been nested into a coarse grid model covering the region between 15°N to 23°N and 85°E to 95°E in the Bay of Bengal to incorporate all the major islands and coastal bending accurately. By applying tidal forcing through the southern open boundary of the coarse grid model appropriate tidal conditions are generated in the problem domain. Numerical experiments are performed with the help of the model to simulate water levels due to tide and surge interaction including air bubble effects associated with the tropical storm of April 1991. The model results are found to be reasonable and the model can be found to simulate higher water levels in the presence of air bubbles.     

An Air of Sharing. A Format of Open Interviewing in a Joint Nordic Project

The five Nordic countries and their respective quality assurance agencies ¹ 1. EVA, FINHEEC, NOKUT, HSV, and the Ministry of Education, Science and Culture of Iceland. have convened annually for over a decade to exchange experiences and discuss issues concerning quality assurance in higher education. In recent years this has resulted in a regional network for the quality assurance agencies (NOQA). During this period, methodological issues have increasingly been emphasised within the network’s activities, and the current article presents and discusses a new format of open interviewing in a setting where the quality assurance work of four higher education institutions were analysed. The openness of the process was the key in providing an opportunity for the higher education institutions to learn from each other.