A parallel microfluidic channel fixture fabricated using laser ablated plastic laminates for electrochemical and chemiluminescent biodetection of DNA

Herein is described the fabrication and use of a plastic multilayer 3-channel microfluidic fixture. Multilayer devices were produced by laser machining of plastic polymethylmethacrylate and polyethyleneterapthalate laminates by ablation. The fixture consisted of an array of nine individually addressable gold or gold/ITO working electrodes, and a resistive platinum heating element. Laser machining of both the fluidic pathways in the plastic laminates, and the stencil masks used for thermal evaporation to form electrode regions on the plastic laminates, enabled rapid and inexpensive implementation of design changes. Electrochemiluminescence reactions in the fixture were achieved and monitored through ITO electrodes. Electroaddressable aryl diazonium chemistry was employed to selectively pattern gold electrodes for electrochemical multianalyte DNA detection from double stranded DNA (dsDNA) samples. Electrochemical detection of dsDNA was achieved by melting of dsDNA molecules in solution with the integrated heater, allowing detection of DNA sequences specific to breast and colorectal cancers with a non-specific binding control. Following detection, the array surface could be renewed via high temperature (95 °C) stripping using the integrated heating element. This versatile and simple method for prototyping devices shows potential for further development of highly integrated, multi-functional bioanalytical devices.     

Integrated carbon fiber electrodes within hollow polymer microneedles for transdermal electrochemical sensing

In this study, carbon fiber electrodes were incorporated within a hollow microneedle array, which was fabricated using a digital micromirror device-based stereolithography instrument. Cell proliferation on the acrylate-based polymer used in microneedle fabrication was examined with human dermal fibroblasts and neonatal human epidermal keratinocytes. Studies involving full-thickness cadaveric porcine skin and trypan blue dye demonstrated that the hollow microneedles remained intact after puncturing the outermost layer of cadaveric porcine skin. The carbon fibers underwent chemical modification in order to enable detection of hydrogen peroxide and ascorbic acid; electrochemical measurements were demonstrated using integrated electrode-hollow microneedle devices.     

A Good Job Is Hard to Find … Until Students Know What They Do Best

Why don't more students use the career center? Shane J. Lopez argues that the traditional career center has not kept up with students’ needs. He suggests that they reimagine themselves as places where students identify and nurture their strengths in order to find fulfilling work and create fulfilling lives.     

Looking Far And Looking Near: Student Insights On History,Language, And Power

ABSTRACT

Three community college students participated in a collaborative student/professor research project to experiment with new ideas in teaching. Under the direction of the instructor, the students studied the South African riots in Soweto, 1976, and how issues of language and power were connected to this event. This investigative process began with video information to introduce students to the key issues, and was then followed by student research and discussion. The students, who were particularly motivated to understand the conflict by relating it to their own linguistic circumstances, worked individually and together to find articles and video sources to support their personal interests and ideas. By reflecting on their experiences and perceptions about language and communication, students were able to become engaged in an unfamiliar historical topic, discover relevant academic information, evaluate sources, and formulate insightful responses.     

Termination Is a Time to Look Back and Move Forward: Farewell From the Editor

We support role changes for educational and psychological consultants who work with children and youth with disabilities as they make important transitions. Principles derived from critical pedagogy and disability studies could provide the theoretical framework for the proposed shift in roles that change the basis on which consulting services are provided. Rather than needs-based services that focus on helping individuals with disabilities cope with deficits, this article supports an empowering person-centered, strength-based orientation tied to perceptions of the individual as competent and complex. We offer recommendations for transition consultants, researchers, and practitioners that could result in more widespread implementation of the principles of critical pedagogy and disability studies.     

The Journal of Educational and Psychological Consultation: A Quarter Century of Contribution,A Future of Challenge and Optimism

The Journal of Educational and Psychological Consultation (JEPC) is beginning its second quarter century of publication. Given the challenges education faced in 1990, when the journal began publishing, and the evolving challenges of 2015, it became important to answer two questions: (1) Has JEPC successfully contributed to the facilitation of service delivery leading to improved outcomes? (2) What major challenges should JEPC consider—in an era of diminishing school budgets, professional autonomy, and social safety nets—to ensure that it can help to meet the needs of struggling learners, their fellow students, and the people and organizations who work on their behalf? To help answer these questions, this article discusses JEPC's contributions to consultation, current and future challenges, and possible directions for meeting the current and future needs of struggling learners, their peers, and all other stakeholders to which JEPC has dedicated itself.     

Application of the Literacy Training Service component of the National Service Training Program in New Bilibid Prison (Philippines)

International Review of Education - In 2000, the government of the Philippines launched its National Service Training Program (NSTP), a compulsory 2-semester course component for all the...     

Scope and Difficulties of the Transmedia Practice. A Case Study on the Spanish PSM System

ABSTRACT

According to a growing volume of academic literature on the subject, transmedia fiction and non-fiction production is now one of the main areas driving innovation in media companies, thanks to the improvements it brings in numerous interconnected fields: narrative and content, management and productive strategy, brand promotion, etc. In this context the article focuses on transmedia linked to public broadcasting companies, starting with a qualitative study based on interviews with expert professionals from thirteen corporations in Spain, one operating at the national level (RTVE) and twelve at the regional level (A Punt Media, CARTV, CCMA, CRTVG, CMM, EITB, EPRTVIB, RTVM, RTPA, RTVA, RTRM and RTVC). The results show how professionals view the concept of transmedia production, its advantages, disadvantages and the challenges it poses, which depend on these corporations’ service and relationship with audiences. As the conclusions state, the keys factors for understanding the professional transmedia strategy of public broadcasting corporations are found in the characteristics of today’s media market, which is suffering from budget cuts and growing competition.     

How Science Teachers DiALoG Classrooms: Towards a Practical and Responsive Formative Assessment of Oral Argumentation

Journal of Science Education and Technology - We present lessons learned from an ongoing attempt to conceptualize, develop, and refine a way for teachers to gather formative assessment evidence...