Quantitation of endogenous inhibitor of milli-CANP in Duchenne muscular dystrophy platelets and lymphocytes: Its implication in regulation

With a view to gain an insight into the regulation of calcium activated neutral protease by its endogenous inhibitor, the inhibitor was quantitated in platelets and lymphocytes of Duchenne muscular dystrophy patients and controls by dot immunoblot procedure using polyclonal antibodies against the inhibitor. A two fold increase in inhibitor levels was observed in lymphocytes but no significant increase in inhibitor levels in platelets from Duchenne muscular dystrophy. These results suggest that different regulatory mechanisms of modulation of CANP operate in these two cell types in Duchenne muscular dystrophy.     

Analysis of Bcl I and Xba I polymorphism in factor VIII gene to detect carriers of haemophilia a in Andhra Pradesh

The efficacy of two intragenic polymorphic markers of factor VIII gene has been examined in Andhra Pradesh population with a view to confirm/revise the strategy for carrier detection that would be precise and economical. The haemophilia A carrier was detected using Bcl I and Xba I polymorphic sites in intron 18 and 22 respectively. The cumulative efficiency of these two sites for detection of carriers is 100% since all 15 families tested were informative for one of these polymorphisms, thus confirming their usefulness for factor VIII gene mutations found in Andhra Pradesh.     

Immunogenicity of formaldehyde and binary ethylenimine inactivated infectious bursal disease virus in broiler chicks

Infectious bursal disease virus （IBDV） was inactivated by two different chemicals-formaldehyde and binary ethylenimine （BEI）. Formaldehyde was used at 0. 1% and 0.2%, while BEI was used at concentrations of 0.001 and 0.002 mol/L. These four vaccines were tested for their efficiency in generating humoral immune response in different groups of broiler chicks. Both BEI-inactivated vaccines gave relatively higher antibody titers and were almost twice as efficient as formaldehyde-inactivated ones.