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291.
Jinghui Luo Bahige G. Abdallah Gregory G. Wolken Edgar A. Arriaga Alexandra Ros 《Biomicrofluidics》2014,8(2)
Isolated mitochondria display a wide range of sizes plausibly resulting from the coexistence of subpopulations, some of which may be associated with disease or aging. Strategies to separate subpopulations are needed to study the importance of these organelles in cellular functions. Here, insulator-based dielectrophoresis (iDEP) was exploited to provide a new dimension of organelle separation. The dielectrophoretic properties of isolated Fischer 344 (F344) rat semimembranosus muscle mitochondria and C57BL/6 mouse hepatic mitochondria in low conductivity buffer (0.025–0.030 S/m) at physiological pH (7.2–7.4) were studied using polydimethylsiloxane (PDMS) microfluidic devices. First, direct current (DC) and alternating current (AC) of 0–50 kHz with potentials of 0–3000 V applied over a channel length of 1 cm were separately employed to generate inhomogeneous electric fields and establish that mitochondria exhibit negative DEP (nDEP). DEP trapping potential thresholds at 0–50 kHz were also determined to be weakly dependent on applied frequency and were generally above 200 V. Second, we demonstrated a separation scheme using DC potentials <100 V to perform the first size-based iDEP sorting of mitochondria. Samples of isolated mitochondria with heterogeneous sizes (150 nm–2 μm diameters) were successfully separated into sub-micron fractions, indicating the ability to isolate mitochondria into populations based on their size. 相似文献
292.
Alexandra Kim 《Curator: The Museum Journal》2011,54(4):403-412
Abstract Kensington Palace, London, has been home to kings and queens, dukes and princesses for 300 years. It was the focus for the tremendous public outpouring of grief following the death of Diana, Princess of Wales in 1997. Recently, the historic building was transformed into “The Enchanted Palace at Kensington,” which sent visitors on a quest through the sumptuous rooms to find seven of the palace’s princesses. On their journey, visitors discovered fantastical, fairy‐tale‐like installations, some of them by the best British fashion designers, and inspired by Kensington Palace’s incredible history. 相似文献
293.
Dirk T. Tempelaar Alexandra Niculescu Bart Rienties Wim H. Gijselaers Bas Giesbers 《The Internet and Higher Education》2012,15(3):161-169
This empirical study investigates students' learning choices for mathematics and statistics in a blended learning environment, composed of both online and face-to-face learning components. The students (N = 730) were university freshmen with a strong diversity in prior schooling and a wide range of proficiency in quantitative subjects. In this context, we investigated the impact that individual differences in achievement emotions (enjoyment, anxiety, boredom, hopelessness) had on students' learning choices, in terms of the intensity of using the online learning mode versus the face-to-face mode. Unlike the general level of learning activities, which is only minimally influenced by achievement emotions, these emotions appear to have a moderately strong effect on a student's preference for online learning. Following this, we explored the antecedents of achievement emotions. Through the use of path-modeling, we conclude that while goal setting behavior only marginally impacts achievement emotions, effort views—a crucial component of the social-cognitive model of implicit theories of intelligence—have a substantial impact on achievement emotions. 相似文献
294.
This article describes initiatives undertaken by one academic medical library to support patrons using handheld mobile devices. These initiatives included efforts to assist patrons with installation and use of mobile information resources, educate librarians regarding content and navigation of mobile apps and websites, and leverage tools to promote the library in a mobile environment. 相似文献
295.
296.
Małgorzata Rymarzak Alexandra den Heijer Monique Arkesteijn Mathilda Du Preez 《Higher Education Quarterly》2023,77(3):447-464
Campus decision makers are increasingly expected to adopt ‘campus innovations’ (affecting real estate and different facilities), not only from the market and demand-led (external campus innovations), but also developed by the university's own scientists (internal campus innovations). The adoption of the latter can be driven and hindered by many unique factors that campus decision makers have not dealt with before. To provide insight into them, qualitative data were collected from 13 out of 14 Dutch research-intensive universities. The results indicate that internal campus innovations are driven by co-creation stimulation, collaborative partnership, transparency and accountability, and local development contribution. Their adoption, however, may be obstructed by barriers embedded in the interaction between campus decision makers and scientists, organizational university context, funds unavailability and innovations' supply-pushed characteristics. An increased understanding of these barriers and the practices to overcome them is crucial for universities' campus decision makers to actively engage in the adoption of internal campus innovations. 相似文献
297.
Our Q method exploration of counselor educators’ perspectives of minimally acceptable cognitive complexity (CC) in trainees at the end of internship yielded two factors: Trainee's Conceptual Integration Ability and Trainee's Ability to Apply Integrated Knowledge. Implications for teaching, supervision, and research pertinent to assessment of and enhancement of CC conclude. 相似文献