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Dmitriy Khodakov Leigh Thredgold Claire E. Lenehan Gunther G. Andersson Hilton Kobus Amanda V. Ellis 《Biomicrofluidics》2012,6(2)
Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional “tag sequence” linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes. 相似文献
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Given projected increases in workplace diversity, an understanding of diversity’s intersection with mentoring is a critical topic in the literature. This article involved an interview with Belle Rose Ragins, one of the world’s leading thinkers on diversity and mentoring in the workplace. After providing an overview of Ragins’ key achievements and contributions to the mentoring literature, the article delved into the interview, which involves four parts: (a) Ragins’ education and early influences; (b) her views on the extant literature as well as insights around future areas for inquiry; (c) her current research interests; and (d) her views on consulting and teaching. 相似文献
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Abstract In this article, we investigate the use of multimedia information and communication technologies (ICTs) in integrated arts projects in two secondary schools. The ways in which teachers and students make sense of the technologies in relation to the dominant discourses of education/technology policy and educational research are explored before considering the implications for curriculum and teacher development. The ICT in the word contrad(ICT)ions is emphasised to represent the ways in which teachers and students' use of multimedia exposes and makes visible problematic issues in relation to literacy, school subjects and pedagogy. 相似文献
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Martha M. Ellis 《Community College Journal of Research & Practice》2013,37(2):73-84
The community college as the entranceway into the baccalaureate degree is becoming a prevalent choice for students. This study was a qualitative approach to understanding attitudes, behaviors, and knowledge acquisition by successful community college transfer students. University students who transferred from a community college and were making successful progress toward completing the baccalaureate degree participated in focus groups to answer questions about the transfer process. Successful transfer students are highly motivated, persistent, engaged, believe they were well prepared by the community colleges for university junior level studies, and have recommendations for community colleges and universities in improving the transfer process. 相似文献
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Jules L. Ellis 《Educational and psychological measurement》2021,81(3):549
This study develops a theoretical model for the costs of an exam as a function of its duration. Two kind of costs are distinguished: (1) the costs of measurement errors and (2) the costs of the measurement. Both costs are expressed in time of the student. Based on a classical test theory model, enriched with assumptions on the context, the costs of the exam can be expressed as a function of various parameters, including the duration of the exam. It is shown that these costs can be minimized in time. Applied in a real example with reliability .80, the outcome is that the optimal exam time would be much shorter and would have reliability .675. The consequences of the model are investigated and discussed. One of the consequences is that optimal exam duration depends on the study load of the course, all other things being equal. It is argued that it is worthwhile to investigate empirically how much time students spend on preparing for resits. Six variants of the model are distinguished, which differ in their weights of the errors and in the way grades affect how much time students study for the resit. 相似文献