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51.
Anmol Goyal Rajendra Prasad Parul Goel Amit Pal Suvarna Prasad Isha Rani 《Indian journal of clinical biochemistry : IJCB》2021,36(4):387
Coronavirus disease 2019 (Covid-19) caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a pandemic disease which has affected more than 6.2 million people globally, with numbers mounting considerably daily. However, till date, no specific treatment modalities are available for Covid-19 and also not much information is known about this disease. Recent studies have revealed that SARS-CoV-2 infection is associated with the generation of thrombosis and coagulopathy. Fundamentally, it has been believed that a diverse array of signalling pathways might be responsible for the activation of coagulation cascade during SARS-CoV-2 infection. Henceforth, a detailed understanding of these probable underlying molecular mechanistic pathways causing thrombosis in Covid-19 disease deserves an urgent exploration. Therefore, in this review, the hypothetical crosstalk between distinct signalling pathways including apoptosis, inflammation, hypoxia and angiogenesis attributable for the commencement of thrombotic events during SARS-CoV-2 infection has been addressed which might further unravel promising therapeutic targets in Covid-19 disease. 相似文献
52.
In this paper, we have presented a non-lithographic embedded template method for rapid and cost-effective fabrication of a selectively permeable calcium-alginate (Ca-alginate) based microfluidic device with long serpentine delay channel. To demonstrate the versatility of the presented method, we have demonstrated two different strategies to fabricate serpentine long delay channels without using any sophisticated microfabrication techniques, in formal lab atmosphere. The procedure presented here, also, enables the preparation of a multilayered microfluidic device with channels of varying dimensions, in a single device without using any sophisticated micromachining instrumentation. In addition, we have also qualitatively studied the diffusion of small and large molecules from a Ca-alginate based microfluidic device and proposed a method to effectively control the out-flow of macro biomolecules from the crosslinked Ca-alginate matrix to create a selectively permeable matrix required for various biological and biomimetic applications, as mentioned in the Introduction section of this work. 相似文献
53.
Vijay S. Bhat Amit Kumar Mandal Bobby Mathew 《Indian journal of clinical biochemistry : IJCB》2012,27(2):202-206
The present report describes the molecular study of HbD Iran (beta) 22 Glu → Gln associated with β-Thalassemia IVS1–5 (G > C) found in India, and the first case in which mutation has been identified using mass spectrometry. Given the apparent ethnic origin and the mobility of the variant hemoglobin at alkaline pH, hemoglobin D-Punjab would be suspected, but HPLC excluded this possibility. Further characterization of hemoglobinopathy was made by using nondenaturing gel electrophoresis and matrix assisted laser desorption ionization mass spectrometry and IVS1–5 being validated by reverse dot blot hybridization followed by sequencing of the β-globin gene. 相似文献
54.
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56.
Vijay S. Bhat S. Lokeshwarappa Amit Kumar Mandal 《Indian journal of clinical biochemistry : IJCB》2015,30(2):238-242
The present case report describes the molecular and proteomic based study of Hb variant HbE associated with β+ thalassemia IVS 1-1 G>T, in a juvenile diabetic patient. Given the ethnic origin and mobility of the variant hemoglobin at alkaline pH, HbE would be suspected. But hematologically and clinically abnormality being detected, HPLC and Electrophoresis not being able to characterize due to retention time and band being in region of HbA2, respectively, further characterization of hemoglobinopathy was made using MALDI and IVS 1-1 G>T being validated by reverse dot blot hybridization. Capillary electrophoresis was also employed in order to separate HbE and HbA2 bands. This case report being first of its kind, wherein a HbE/β+ thalassemia has been characterized using multiple techniques. 相似文献
57.
Matthew B. Byrne Lisa Trump Amit V. Desai Lawrence B. Schook H. Rex Gaskins Paul J. A. Kenis 《Biomicrofluidics》2014,8(4)
Diffusion of
autocrine and paracrine signaling molecules allows cells to
communicate in the absence of physical contact. This chemical-based, long-range
communication serves crucial roles in tissue function, activation of the immune system,
and other physiological functions. Despite its importance, few in vitro
methods to study cell-cell signaling through paracrine factors are available today. Here,
we report the design and validation of a microfluidic platform that enables (i) soluble molecule-cell and/or
(ii) cell-cell paracrine signaling. In the microfluidic platform, multiple cell
populations can be introduced into parallel channels. The channels are separated by arrays
of posts allowing diffusion of paracrine molecules between cell
populations. A computational analysis was performed to aid design of the microfluidic platform.
Specifically, it revealed that channel spacing affects both spatial and temporal
distribution of signaling molecules, while the initial concentration of the signaling
molecule mainly affects the concentration of the signaling molecules excreted by the
cells. To validate the microfluidic platform, a model system composed of the
signaling molecule lipopolysaccharide, mouse macrophages, and engineered human embryonic
kidney
cells was introduced into the platform. Upon diffusion from the first
channel to the second channel, lipopolysaccharide activates the macrophages which begin to
produce TNF-α. The TNF-α diffuses from the second channel to the third channel to
stimulate the kidney
cells, which express green fluorescent protein (GFP) in response. By
increasing the initial lipopolysaccharide concentration an increase in fluorescent
response was recorded, demonstrating the ability to quantify intercellular communication
between 3D cellular constructs using the microfluidic platform reported
here. Overall, these studies provide a detailed analysis on how concentration of the
initial signaling molecules, spatiotemporal dynamics, and inter-channel spacing affect
intercellular
communication. 相似文献
58.
Ghizal Fatima Siddharth Kumar Das Abbas Ali Mahdi Nar Singh Verma Faizan Haider Khan Amit Mani Kumar Tiwari Tabrez Jafer Baby Anjum 《Indian journal of clinical biochemistry : IJCB》2013,28(2):181-184
Fibromyalgia syndrome (FMS) patients have disturbed sleep patterns which may lead to altered circadian rhythm in serum cortisol secretion. The aim of this study was to assess circadian changes, if any, in serum cortisol levels in female patients with FMS. Cortisol levels were estimated every 6 h during 24 h period; in 40 female patients satisfying ACR criteria for FMS (Age 36.4 ± 9.9), and 40 healthy females without FMS (Age 33.8 ± 11.1). A significant difference in the night time serum cortisol level was observed among the patients and control groups (patients, 12.9 ± 9.7 controls 5.8 ± 3.0; p < 0.01). However, no significant difference was found in serum cortisol levels in patients and control groups in the morning (patients, 28.4 ± 13.2 controls, 27.6 ± 14.5; p > 0.05), afternoon (patients, 14.4 ± 5.6 controls, 14.0 ± 6.6; p > 0.05) and evening hours (patients, 10.9 ± 5.8 controls, 8.9 ± 3.6; p > 0.05). It could be concluded that there is an abnormality in circadian secretion of cortisol in female FMS patients. 相似文献
59.
This study delves into the motivations, symbolic rewards, and experiences of Filipinos involved in the creation of a magazine catering to the Filipino migrant workers’ community in Israel. Although practices of resistance are the prevailing framework within research about diasporic media, this paper offers another perspective of the construction of a subjugated minority's sphericule. Power (or lack thereof) is not necessarily a basic force of motivation. Participation in cultural production is not perceived as a journalistic endeavor by Filipinos, but a rare and crucial opportunity to be heard, to have a voice, to win over coerced living circumstances of alienation, solitude, and hard work. In this context they are not ‘just the caregiver,’ but accomplished writers, winners of competitions, and recipients of respect. Empowerment is derived from pleasure; it is grounded in recreational gratifications and a sense of mission that have no political dimensions. 相似文献
60.
Chu-Feng Liu Weng-Hang Leong Kangwei Xia Xi Feng Amit Finkler Andrej Denisenko Jrg Wrachtrup Quan Li Ren-Bao Liu 《国家科学评论(英文版)》2021,8(5)
Nitrogen-vacancy (NV) centers in diamond are promising quantum sensors because of their long spin coherence time under ambient conditions. However, their spin resonances are relatively insensitive to non-magnetic parameters such as temperature. A magnetic-nanoparticle-nanodiamond hybrid thermometer, where the temperature change is converted to the magnetic field variation near the Curie temperature, were demonstrated to have enhanced temperature sensitivity () (Wang N, Liu G-Q and Leong W-H et al. Phys Rev X 2018; 8: 011042), but the sensitivity was limited by the large spectral broadening of ensemble spins in nanodiamonds. To overcome this limitation, here we show an improved design of a hybrid nanothermometer using a single NV center in a diamond nanopillar coupled with a single magnetic nanoparticle of copper-nickel alloy, and demonstrate a temperature sensitivity of . This hybrid design enables detection of 2 mK temperature changes with temporal resolution of 5 ms. The ultra-sensitive nanothermometer offers a new tool to investigate thermal processes in nanoscale systems. 相似文献