Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

权力话语的镜像表现——论中国"十七年"电影英雄叙事的性别话语

“十七年”电影中的性别话语作为一种文化话语是一种功能、运动和关系,其意义也在这些功能、运动和关系中产生、发展和变化,英雄叙事功能本身就构成一个复杂的话语系统。因此,“十七年”电影英雄叙事的性别话语研究就必须结合其他话语系统如意识形态、历史等,才能充分考虑背后的权力关系,即叙事运作的策略性关系。     

浅析当前徐州市场钾肥生产与销售状况

钾是植物三大必要营养元素之一,是我国粮食和农产品的重要支柱。2011年,受进口钾肥价格大幅上涨等因素影响,国内进口钾肥企业和国内主要生产企业出现压单、囤货等现象,可能影响农业生产资料运转,加大农业生产成本,挫伤农民种粮积极性,并进一步影响农民增收。     

The relative age effect on physical fitness in preschool children

ABSTRACT

The aim of the present study was to investigate the existence of a relative age effect (RAE) on physical fitness of preschoolers. Anthropometry and physical fitness were assessed in 3147 children (3–5 years old) using the PREFIT battery. Based on the birth year, participants were divided into 3year groups (3-, 4- and 5-years). Within each year group, 4quarter groups were created: quarter 1, preschoolers born from January to March; quarter 2, from April to June; quarter 3, from July to September; quarter 4, from October to December. The MANCOVA analysis revealed a main effect of year group (Wilks’ λ = 0.383; F_10,5996 = 369.64; p < 0.001, η_p ² = 0.381) and of quarter (Wilks’ λ = 0.874; F_15,8276.6 = 27.67; p < 0.001; η_p ² = 0.044) over the whole battery of tests. To the best of our knowledge, this is the first study to report the existence of RAE at the preschool stage. In general, performance improved as the relative age increased (i.e., those born in quarter 1 performed better than those in the other quarters). Individualization strategies should be addressed within the same academic year not only in elementary or secondary years but also in preschoolers.     

Higher Education as a Platform for Cross‐Cultural Transition: the Case of the First Educated Bedouin Women in Israel

This article examines two groups of Bedouin women who studied in different cultural spaces. The first group, due to a lack of high schools in the Negev (during the 1970s), were obliged to leave the village to study and reside in boarding schools in the central and northern regions of Israel. These women returned to their society of origin after the completion of their academic studies. The second group went to Jewish universities in a town near their homes (Beersheba), attending daily and returning home each night to patriarchal control. The article examines the experience of these pioneers in higher education as a form of cultural transition and internal immigration, with an emphasis on the unique characteristics of each of the two groups. Going out to gain higher education is seen in Bedouin society as a form of immigration, and the first educated women therefore became a type of immigrant.     

身在佛门心系红尘——妙玉情感矛盾透析

自动走向佛门是精神解脱,被动走向佛门是精神禁锢。妙玉“身在佛门,心系红尘”,其情感矛盾的变化折射出她人生思想的变化历程,反映了佛性与人性在其心中地位的转换。人性的复醒,使妙玉对红尘生活的向往与日俱增,她背负着沉重的精神枷锁,步履艰难地迈向世俗世界。妙玉在人性与佛性的门缝中呻吟、挣扎,最终被佛性与人性两扇大门挤扁了,磨碎了。