Membrane-integrated microfluidic device for high-resolution live cell imaging

The design and fabrication of a membrane-integrated microfluidic cell culture device (five layers,≤500 μm total thickness) developed for high resolution microscopy is reported here. The multi-layer device was constructed to enable membrane separated cell culture for tissue mimetic in vitro model applications and pharmacodynamic evaluation studies. The microdevice was developed via a unique combination of low profile fluidic interconnect design, substrate transfer methodology, and wet silane bonding. To demonstrate the unique high resolution imaging capability of this device, we used oil immersion microscopy to image stained nuclei and mitochondria in primary hepatocytes adhered to the incorporated membrane     

Decreasing the proportion of self-control trials during the acquisition period does not compromise the learning advantages in a self-controlled context

The present experiment examined the learning effects of participants self-controlling their receipt of knowledge of results (KR) on all or half of their acquisition trials (50%). For participants who were provided 50% self-control, the first half of their acquisition period consisted of receiving KR on all trials, or according to a faded-KR schedule. Participants practiced a sequential timing task. The results showed that independent of practice condition, participants who self-controlled their KR during the acquisition period demonstrated superior performance compared to the respective yoked conditions in the retention and transfer portion of the experiment. These results extend previous research by suggesting that decreasing the proportion of self-control trials does not compromise learning in a self-controlled context.     

The force-time profile of elite front crawl swimmers

In this study, we used recently developed technology to determine the force-time profile of elite swimmers, which enabled coaches to make informed decisions on technique modifications. Eight elite male swimmers with a FINA (Federation Internationale de Natation) rank of 900+ completed five passive (streamline tow) and five net force (arms and leg swimming) trials. Three 50-Hz cameras were used to video each trial and were synchronized to the kinetic data output from a force-platform, upon which a motorized towing device was mounted. Passive and net force trials were completed at the participant's maximal front crawl swimming velocity. For the constant tow velocity, the net force profile was presented as a force-time graph, and the limitation of a constant velocity assumption was acknowledged. This allowed minimum and maximum net forces and arm symmetry to be identified. At a mean velocity of 1.92+0.06 m s?1, the mean passive drag for the swimmers was 80.3+4.0 N, and the mean net force was 262.4+33.4 N. The mean location in the stroke cycle for minimum and maximum net force production was at 45% (insweep phase) and 75% (upsweep phase) of the stroke, respectively. This force-time profile also identified any stroke asymmetry.     

The possibility of deliberate norm-adherence in AI

Ethics and Information Technology - Moral agency status is often given to those individuals or entities which act intentionally within a society or environment. In the past, moral agency has...     

Tweet Up? Examining Twitter's Impact on Social Capital and Digital Citizenship in Higher Education

Twitter is a highly accessible communication venue that is embraced by great numbers of traditionally aged and ethnically diverse college students. Danielle Morgan Acosta challenges us to turn our research and teaching attention to the potential of Twitter to support student learning.     

Peer Play Interactions and Learning for Low-Income Preschool Children: The Moderating Role of Classroom Quality

Research Findings: The present study examined the degree to which the association between interactive peer play and academic skills was dependent upon the level of classroom quality for a representative sample of culturally and linguistically diverse urban Head Start children (N = 304 children across 53 classrooms). Peer play interactions within the classroom were assessed by teacher assistants in the fall of the year; observations of the quality of classroom instructional, emotional, and organizational support were conducted in the middle of the year; and norm-referenced direct assessments of literacy, language, and mathematics skills were administered in the spring. Findings from multilevel models indicated that disruptive and disconnected peer play behaviors early in the preschool year were associated with lower literacy and language skills regardless of classroom quality. However, interactive peer play early in the year was associated with higher mathematics outcomes when children were enrolled in classrooms characterized by high instructional support. Practice or Policy: Implications for early childhood research, practice, and policy are discussed.     

An Investigation of How a Physics Professional Development Course Influenced the Teaching Practices of Five Elementary School Teachers

This paper reports the results of an investigation of how a professional development content course based on the Physics and Everyday Thinking (PET) curriculum affected the teaching practices of five case study elementary school teachers. The findings of this study highlight different ways that teachers use what they learn in content courses to teach science to elementary children. While some teachers transferred pedagogical practices along with the content, others transformed the content to be useful in already existing pedagogical frameworks, and still others show little or no evidence of transfer. The range of transfer is explained by considering how each teacher interacted with the learning context (the PET curriculum) and their initial ideas about teaching science.     

In vitro antioxidant activity and potential inhibitory action against α-glucosidase of polysaccharides from fruit peel oftea (Camellia sinensis L.)简

The conditions for extracting polysaccharides from tea (Camellia sinensis L.) fruit peel (TFPPs) were studied. Three parameters (temperature, time, and liquid/solid ratio) affecting the extraction of TFPP were optimized using response surface methodology (RSM). Under the optimized conditions, the yield of TFPP was predicted to be 4.98%. The physicochemical properties, in vitro antioxidant activities, and inhibitory effects on α-glucosidase of fractionated TFPPs (TFPP-0, TFPP-20, TFPP-40, and TFPP-60) were investigated. We found that the TFPPs were all acid protein-bound heteropolysaccharides, although with different chemical compositions. They had not only remarkable scavenging activity on 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and reducing activity, but also excellent inhibitory potential against α-glucosidase in vitro. Our results suggest that tea fruit peel could be treated as a potential bioresource for the development of polysaccharide antioxidants.     

A Behind‐the‐Scenes Guide to School‐Based Research

Schools are an important context for both basic and applied scientific research. Unlike the laboratory, however, the physical and social conditions of schools are not under the exclusive control of scientists. In this article, we liken collecting data in schools to putting on a theatrical production. We begin by describing the large cast of characters whose collaborative efforts make school‐based research possible. Next, we address the critics, including the university Institutional Review Board (IRB) and school administrators, whose feedback often improves the final study design. We then turn our attention to set building, stage directions, and rehearsals—key steps in the iterative process of refining study procedures. We end with a discussion of the day of data collection itself and activities that take place after the curtain drops. Throughout, we make recommendations based on our recent experience collecting data at several high schools.