Performance evaluation of the high sensitive troponin I assay on the Atellica IM analyser

IntroductionThe Fourth Universal Definition of Myocardial Infarction Global Taskforce recommends the use of high sensitive troponin (hs-Tn) assays in the diagnosis of acute myocardial infarction. We evaluated the analytical performance of the Atellica IM High-sensitivity Troponin I Assay (hs-TnI) (Siemens Healthcare Diagnostics Inc., Tarrytown, USA) and compared its performance to other hs-TnI assays (Siemens Advia Centaur, Dimension Vista, Dimension EXL, and Abbott Architect (Wiesbaden, Germany)) at one or more sites across Europe.Materials and methodsPrecision, detection limit, linearity, method comparison, and interference studies were performed according to Clinical and Laboratory Standards Institute protocols. Values in 40 healthy individuals were compared to the manufacturer’s cut-offs. Sample turnaround time (TAT) was examined.ResultsImprecision repeatability CVs were 1.1–4.7% and within-lab imprecision were 1.8–7.6% (10.0–25,000 ng/L). The limit of blank (LoB), detection (LoD), and quantitation (LoQ) aligned with the manufacturer’s values of 0.5 ng/L, 1.6 ng/L, and 2.5 ng/L, respectively. Passing-Bablok regression demonstrated good correlations between Atellica IM analyser with other systems; some minor deviations were observed. All results in healthy volunteers fell below the 99th percentile URL, and greater than 50% of each sex demonstrated values above the LoD. No interference was observed for biotin (≤ 1500 µg/L), but a slight bias at 5.0 g/L haemoglobin and 50 ng/L Tn was observed. TAT from was fast (mean time = 10.9 minutes) and reproducible (6%CV).ConclusionsReal-world analytical and TAT performance of the hs-TnI assay on the Atellica IM analyser make this assay fit for routine use in clinical laboratories.     

How the Role of Computing is Driving New Genetics' Public Policy

In this paper we will examine some ethical aspects of the role that computers and computing increasingly play in new genetics. Our claim is that there is no new genetics without computer science. Computer science is important for the new genetics on two levels: (1) from a theoretical perspective, and (2) from the point of view of geneticists practice. With respect to (1), the new genetics is fully impregnate with concepts that are basic for computer science. Regarding (2), recent developments in the Human Genome Project (HGP) have shown that computers shape the practices of molecular genetics; an important example is the Shotgun Method's contribution to accelerating the mapping of the human genome. A new challenge to the HGP is provided by the Open Source Philosophy (I computer science), which is another way computer technologies now influence the shaping of public policy debates involving genomics.     

La influencia del estilo cognitivo «Dependencia-Independencia de campo» en la resolución de dos problemas de física

Resumen

Este artículo trata de la influencia que tiene, en la actuación formal de los adolescentes, el estilo cognitivo. En este caso, el estilo cognitivo estudiado es la “Dependencia-Independencia de campo”, y las pruebas formales utilizadas son dos problemas de física en los que está implicado el esquema de control de variables. Los resultados obtenidos avalan la hipótesis de que, en este tipo de tareas, son los sujetos dependientes de campo los que tienen mayores dificultades para actualizar su competencia formal debido, fundamentalmente, a la dificultad que presentan para disociar unos factores de otros, sobre todo cuando éstos se ofrecen perceptivamente muy ligados.     

Leptotrichia hongkongensis sp. nov., a novel Leptotrichia species with the oral cavity as its natural reservoir

A straight, non-sporulating, Gram-variable bacillus (HKU24T) was recovered from the blood culture of a patient with metastatic breast carcinoma. After repeated subculturing in BACTEC Plus Anaerobic/F blood culture broth, HKU24T grew on brucella agar as non-hemolytic, pinpoint colonies after 96 h of incubation at 37 °C in an anaerobic environment and aerobic environment with 5% CO2. Growth was enhanced with a streak of Staphylococcus aureus. HKU24T was non-motile and catalase-negative, but positive for alkaline phosphatase, β-glucosidase, and α-glucosidase. It hydrolyzed phenylphosphonate and reduced resazurin. 16S rRNA, groEL, gyrB, recA, and rpoB sequencing showed that HKU24T occupies a distinct phylogenetic position among the Leptotrichia species, being most closely related to Leptotrichia trevisanii. Using HKU24T groEL, gyrB, recA, and rpoB gene-specific primers, fragments of these genes were amplified from one of 20 oral specimens. Based on phenotypic and genotypic characteristics, we propose a new species, Leptotrichia hongkongensis sp. nov., to describe this bacterium.     

Impact case studies: what accounts for the need for numbers in impact evaluation?

ABSTRACT

The UK Research Excellence Framework (REF) introduced impact as an indicator in the evaluation of higher education research quality in 2014. Impact case studies (ICS) are the basis of this evaluation. ICS use narrative explanations of how research causes ‘benefits to society’. This article analyses the evolving roles of the ICS and the relationships of ICS with various stakeholders. It draws on insights from legitimacy theory, Porter’s analysis of the social meaning of objectivity, and the insight from economist Alan Goodhart on the dysfunctional transformation of indicators into targets. It highlights the challenges to ICS evaluators and shows how a spiral of mistrust can both undermine but also cause demand for numbers. It explores whether impact narratives represent an escape from dominant research evaluation metrics and concludes that they do not.     

Something old,not much new,and a lot borrowed: philanthropy,business, and the changing roles of government in global education policy networks

This paper focuses on the role of governments in contemporary networked political configurations. Such networks constitute policy communities, usually based upon shared conceptions of social problems and their solutions. By enabling social, political, and economic connections at local, regional, national, and international levels, such networks become key policy players as well as a policy technology in different spaces. More specifically, the paper is organised around three policy frameworks in the field of education. Each framework is based on a ‘network-case’. In the first framework, governments represent the main driver for political change in legislating a landscape that creates the conditions for networks to develop around different aspects within the public sphere (e.g. organisation, co-funding, delivery, etc.). The second policy framework focuses on the activities of an already organised network in order to engage with existing political configurations as a ‘political actor’ in its own right, what could be called ‘governing with/alongside networks’. The third policy framework focuses on instances where the network operates directly as a ‘state-maker’.     

Changing Criterion Designs: Integrating Methodological and Data Analysis Recommendations

Abstract

Changing criterion designs (CCD) are single-case experimental designs that entail a step-by-step approximation of the final level desired for a target behavior. Following a recent review on the desirable methodological features of CCDs, the current text focuses on an analytical challenge: the definition of an objective rule for assessing the correspondence between criterion levels and behavior. We review extensively applied research and methodological recommendations for establishing experimental control, such as the range-bound CCD, and integrate them into a proposal for a formal expression of an objective rule. We illustrate how this proposal can lead to establishing the acceptable level of behavior prior to gathering the intervention phase data and how it leaves space for professional judgment. A discussion of software implementing the suggested proposal is included.     

“Don’t give up on me”: critical mentoring pedagogy for the classroom building students’ community cultural wealth

This one-year ethnographic case study focused on students of color from a West Coast High School who faced a variety of academic challenges. Collectively, they shared perspectives on school improvement, and among the recommendations was the importance of mentorship in the classroom to develop students’ aspirational, navigational, and informational capital for academic resiliency, high expectations, and success. This article highlights the perspectives of multiple stakeholders, such as students and teachers, on the qualities of mentorship practices. Using these data, a framework was developed to promote mentoring as an interrelated process in classroom instruction to ignite a new perspective on school reform. In this particular context, this study concludes that these students of color viewed mentorship as a critical component that was often missing in their high school careers. In an effort to improve students’ experiences in the classroom, this article argues that teachers must develop critical mentoring skills, beyond what is traditionally considered as mentoring services, as a pedagogical tool to assist students to overcome their academic challenges and achieve school success.