Traceable clonal culture and chemodrug assay of heterogeneous prostate carcinoma PC3 cells in microfluidic single cell array chips

Cancer heterogeneity has received considerable attention for its role in tumor initiation and progression, and its implication for diagnostics and therapeutics in the clinic. To facilitate a cellular heterogeneity study in a low cost and highly efficient manner, we present a microfluidic platform that allows traceable clonal culture and characterization. The platform captures single cells into a microwell array and cultures them for clonal expansion, subsequently allowing on-chip characterization of clonal phenotype and response against drug treatments. Using a heterogeneous prostate cancer model, the PC3 cell line, we verified our prototype, identifying three different sub-phenotypes and correlating their clonal drug responsiveness to cell phenotype.     

Shared Governance among the new Majority: Non-Tenure Track Faculty Eligibility for Election to University Faculty Senates

Non-tenure track faculty members (NTTF) constitute what has been referred to by scholars as the new faculty majority. The growing numbers of NTTF have led to debates about the role they should play in shared governance. Currently, however, an overall lack of empirical knowledge exists regarding the status of their involvement in institutional governance. Using data from highest research activity doctoral universities, this study investigated current standards related to NTTF eligibility for election to institution-wide faculty senates. We also explored what these faculty governance standards and criteria reveal about the status and position of NTTF within the professoriate.     

A Conceptual Framework for Learning to Teach Secondary Mathematics: A Situative Perspective

This paper offers for discussion and critique a conceptual framework that applies a situative perspective on learning to the study of learning to teach mathematics. From this perspective, such learning occurs in many different situations -- mathematics and teacher preparation courses, pre-service field experiences, and schools of employment. By participating over time in these varied contexts, mathematics teachers refine their conceptions about their craft -- the big ideas of mathematics, mathematics-specific pedagogy, and sense of self as a mathematics teacher. This framework guides a research project that traces the learning trajectories of teachers from two reform-based teacher preparation programs into their early teaching careers. We provide two examples from this research to illustrate how this framework has helped us understand the process of learning to teach. This revised version was published online in July 2006 with corrections to the Cover Date.     

Sexual consent in K–12 sex education: an analysis of current health education standards in the United States

In the USA, universities have recently developed policies and programmes on sexual consent education. But waiting until students enroll in higher education may be too late to begin this work. To examine the extent that K–12 health education standards promote sexual consent education, we conducted a pilot study and found that only two of eighteen states explicitly mentioned sexual consent in their health education standards. Using a small sample (n = 4 states), we then identified four themes as making implicit reference to sexual consent: communication skills, decision making, personal space and interpersonal relationships. Finally, in a robust sample (n = 18 states), we conducted a content analysis of published standards regarding these themes related to sexual consent. Our analysis suggests that sexual consent is likely not discussed in sex education at K–12 schools. We recommend the more explicit inclusion of sexual consent in health education curricula via the identified themes that already exist in most or all standards, emphasising the importance of teaching young people about the nuances of sexual consent and its communication before they become sexually active.     

The influence of brief stimuli uncorrelated with reinforcement on choice between variable-ratio schedules

Experiment 1 investigated the behavior of rats trained to leverpress on a concurrent variable ratio (VR) 30 VR-30 schedule with a brief, 500-msec, light occurring at the midpoint of the ratio on one of the levers. Higher response rates were recorded on the lever associated with this stimulus, a finding that paralleled the effect produced by inserting primary reinforcement at the midpoint (i.e., by training on a concurrent VR-30 VR-15 schedule). Similar results were found in Experiment 2 using a concurrent VR-20 VR-20 schedule with a 2-sec visual stimulus presented midway through one of the components. In addition, a brief stimulus inserted midway through the VR-20 component of a concurrent VR-20 VR-10 schedule retarded the development of a difference in response rates between the components relative to a VR-20 VR-10 group lacking the signal. In Experiment 3, multiple VR VR schedules were used. Again, the response rate was higher in the component that had the added stimulus or, for a second group of subjects, on the component with the smaller response requirement. Probe-choice trials revealed a preference for the component that generated the higher rate in both groups. Presenting a stimulus partway through a ratio appears to reduce the effect on response rate and choice of a large ratio value.     

Barriers to the Success of Affirmative Consent Initiatives: An Application of the Social Ecological Model

ABSTRACT

In response to evidence that one in five college women are sexually assaulted, institutions of higher education have started adopting affirmative consent policies. Affirmative consent must be voluntarily and explicitly communicated—verbally or nonverbally. A recently published article highlighted barriers to the success of affirmative consent initiatives at the intrapersonal level. To extend this discussion, we identified barriers at each level of the Social Ecological Model. In our commentary, we discuss social determinants that are particularly relevant for campus sexual assault. There are elements in young people's micro-, meso-, exo-, and macrosystems that hinder their adoption of affirmative consent practices. We focus on gender as an aspect of the macrosystem that is a formidable barrier to the success of affirmative consent initiatives, influencing each of the other levels. Finally, we discuss how sexuality education might begin to address the social determinants of sexual assault.     

Designing your web site for instructional effectiveness and completeness: First steps

TechTrends - Developing and publishing Web sites is becoming almost trivially easy today, and there are many sites on the Web nowadays that purport to be instructional in one way or another. In...