Possible Role of microRNA-122 in Modulating Multidrug Resistance of Hepatocellular Carcinoma

Hepatocellular carcinoma (HCC) is a hypervascular primary liver cancer characterized by rapid progression, besides, resistance to traditional chemotherapeutic agents. It has been shown that microRNAs play critical roles in regulation of tumor cell sensitivity to drugs through modulating the expression of genes involved in drug transport. The present study investigated whether restoration of miR-122 in HCC cells could alter the cell cycle distribution and the expression of multidrug resistance (MDR)-related genes (ABCB1, ABCC1, ABCG2 and ABCF2). After overexpression of miR-122 in HepG2 cells treated or untreated with doxorubicin doses, total RNAs and protein extracts were isolated for application of QRT-PCR and western blotting techniques. Moreover, cell cycle distribution was monitored by flow cytometry. Our results revealed that, the over expression of miR-122 in HepG2 cells treated or untreated with doxorubicin could modulate the sensitivity of cells to chemotherapeutic drug through downregulation of MDR-related genes, ABCB1 and ABCF2. Interpretation of cell cycle distribution revealed that, the anti-proliferative effect of miR-122 is associated with the accumulation of cells in G₀/G1 phase. Moreover, treatment with miR-122 and doxorubicin resulted in high percentage of HCC cells in G₀/G1 phase. Taken together, our findings revealed that, overexpression of miR-122 inhibited HCC cell growth by inducing cell cycle arrest and this arrest is associated with down-regulation of MDR-related genes.     

Learning orientations of IT higher education students in UAE University

This research examines the learning preferences of students in UAE University (UAEU). The uniqueness of this research emanates from the fact that no prior research examined this area from the UAE’s perspective. Thus, this research embarks on the fact that student learning strategies vary from one country to another due to many factors. This research utilizes six learning strategies extended from the literature and attempts to examine their importance on UAEU students using survey research. The selected learning strategies were students” motivation, time-poorness, mastery effort, assessment focus, competitiveness, and listening. This research provided interesting insights and contrasts pertaining to the learning strategies of UAEU students. Implications are discussed highlighting different theoretical as well as professional contributions and contentions and portrayed a path where pending issues could be addressed by future research.     

Family contexts of television

Educational technology research and development -     

Dargah libraries in India: a comparative study

This paper relates to the difficulty in retrieving precise information from big repositories of magazine articles in full text, and proposes an Extended Markup Language (XML) vocabulary for improving retrieval rates. The hypothesis tested was as follows: Magazine articles marked up with an XML vocabulary, indexed only by selected parts, give more precise search results than the same search using full text index.

The study was exploratory with the following characteristics: 29 magazine articles were tested for results, 8 scholars were interviewed for defining 23 search strategies and evaluating results. The data showed that precision improved from 40.72% with full text search to 62.84% using XML markup and searching only in specific labels.

Revision of the vocabulary and more testing has to be done by the library and information science community in order to obtain a valid vocabulary and provide more research results. Cultural characteristics and politics of librarians and information managers’ community are as important as technical issues in order to consider any technical proposal to be implemented successfully to achieve interoperability.     

Pre-service teachers’ and teacher-educators’ experiences and attitudes toward using social networking sites for collaborative learning

Extensive use of social networking sites by students and teachers makes educators and researchers to think whether they can be incorporated in instructional process to facilitate students’ learning. This survey-based study records and examines Pakistani pre-service teachers’ and teacher-educators’ current uses of Facebook, and their attitudes toward using Facebook for collaborative learning, and to see if there is a significant relationship between participants’ intensity of Facebook use and their attitude toward instructional use of Facebook. Results of the study indicated that pre-service teachers’ and teacher-educators’ motives for their current Facebook use are mainly limited for social purposes. Pre-service teachers’ use of Facebook is more intensified than the use of Facebook by teacher-educators. Further, pre-service teachers showed more positive attitudes toward using Facebook for collaborative learning than the attitudes of their faculty. Findings also indicated a positive relationship between participants’ Facebook intensity and their attitudes toward its use for collaborative learning.     

The major players in adaptive immunity

How do we remain healthy, for the most parts, in the midst of an environment teeming with opportunistic and infectious microbes, potential carcinogens and allergens? The fact is that our immune system, by and large, does a fine job in protecting us. It is therefore important to understand the organization of the immune network, which is broadly categorized into two groups: innate and adaptive. Cells involved in innate immunity are the first to come into contact with invading microbes, similar to the border security force, and respond rapidly but in a non-specific manner. On the other hand, the cells involved in adaptive immunity are slower to respond but act in a very specific manner. Though the primary response is slow, the secondary response is much faster and demonstrates memory. This article will focus on some important features and key players involved in the adaptive immune response. The first part deals with the humoral immune response mediated mainly by immunoglobulins produced by the B cells. The second part deals with T cells, the Major Histocompatibility Complex (MHC)-encoded molecules, and Recombination Activating Genes (RAG) responsible for generating diverse B-cell receptors (BCR) and T-cell receptors (TCR). With the advent of newer and smarter infectious agents, it is important to understand the working of the immune network as more research in this area may facilitate the development of better protective strategies.     

Bioconversion of low quality lignocellulosic agricultural waste into edible protein by <Emphasis Type="Italic">Pleurotus sajor-caju</Emphasis> (Fr.) singer

Pleurotus sajor-caju (Fr.) Singer was cultivated on selected agro wastes viz. cotton stalks, groundnut haulms, soybean straw, pigeon pea stalks and leaves and wheat straw, alone or in combinations. Cotton stalks, pigeon pea stalks and wheat straw alone or in combination were found to be more suitable than groundnut haulms and soybean straw for the cultivation. Organic supplements such as groundnut oilseed cake, gram powder and rice bran not only affected growth parameters but also increased yields. Thus bioconversion of lignocellulosic biomass by P. sajor-caju offers a promising way to convert low quality biomass into an improved human food.