Deletions in the genomes of fifteen inbred mouse lines and their possible implications for fat accumulation

Copy number variants (CNVs) are pieces of genomic DNA of 1000 base pairs or longer which occur in a given genome at a different frequency than in a reference genome. Their importance as a source for phenotypic variability has been recognized only in the last couple of years. Chromosomal deletions can be seen as a special case of CNVs where stretches of DNA are missing in certain lines when compared to the reference genome of the mouse line C57BL/6, for example. Based upon more than 8 million single nucleotide polymorphisms (SNPs) in the fifteen inbred mouse lines which were determined in a whole genome chip based resequencing project by Perlegen Sciences, we detected 20166 such long chromosomal deletions. They cover altogether between 4.4 million and 8.8 million base pairs, depending on the mouse line. Thus, their extent is comparable to that of SNPs. The chromosomal deletions were found by searching for clusters of missing values in the genotyping data by applying bioinformatics and biostatistical methods. In contrast to isolated missing values, clusters are likely the consequence of missing DNA probe rather than of a failed hybridization or deficient oligos. We analyzed these deletion sites in various ways. Twenty-two percent of these deletion sites overlap with exons; they could therefore affect a gene's functioning. The corresponding genes seem to exist in alternative forms, a phenomenon that reminds of the alternative forms of mRNA generated during gene splicing. We furthermore detected statistically significant association between hundreds of deletion sites and fat weight at the age of eight weeks.     

Body dissatisfaction in women’s artistic gymnastics: A longitudinal study of psychosocial indicators

Body dissatisfaction is prevalent in women’s artistic gymnastics (WAG). Cross-sectional research points to social and individual risk factors, however it does not account for potential changes in body dissatisfaction during an athletic season. This study aimed to determine how gymnasts’ body dissatisfaction, risk factors for eating disorders, media internalisation, perfectionism and mood state change during pre-competition, competition and post-competition seasons and to identify how these psychosocial indicators impact on body dissatisfaction during the athletic year. The sample consisted of 20 Brazilian elite women’s artistic gymnasts aged 10–16 years. Data were obtained from a 9-month study using: Body Shape Questionnaire (BSQ); Eating Attitude Test-26; Sociocultural Attitudes Towards Appearance Questionnaire-3 (SATAQ-3); Multidimensional Perfectionism Scale (MPS); Brunel Mood Scale (BRUMS) and triceps and subscapular skinfolds. Body dissatisfaction was higher during the competition season and disordered eating, perfectionism and vigour values were higher in the pre-competition season. Disordered eating has been found as the strongest predictor of body dissatisfaction during all seasons, and mood state partly contributed to body dissatisfaction in the competitive season. Stakeholders should understand that body dissatisfaction and the prevalence of disordered eating may change over time.     

Children's overestimation of performance across age,task, and historical time: A meta-analysis

Children tend to overestimate their performance on a variety of tasks and activities. The present meta-analysis examines the specificity of this phenomenon across age, tasks, and more than five decades of historical time (1968–2021). Self-overestimation was operationalized as the ratio between children's prospective self-estimates of task performance and their actual (i.e., objectively measured) task performance. A total of 246 effect sizes from 43 published articles were analyzed (4277 participants; 49.6% girls; sample mean ages range from 4 to 12; 86.0% of studies conducted in North America or Europe). Children's self-overestimation was robust across tasks, with their estimates of performance being 1.3 times their actual performance. In addition, children's self-overestimation decreased with sample age and increased with the year of data collection.     

Public agricultural extension workers as boundary workers: identifying sustainability perspectives in agriculture using Q-methodology

Purpose: To provide farmers with access to salient knowledge on sustainability that could contribute positively to farmers’ livelihoods, there is a need for knowledge facilitators. This paper examines the role of public extension workers as boundary workers in Indonesia on sustainable agriculture and challenges around them.

Design/methodology/approach: To identify sustainability perspectives, this research uses Q-methodology which analyzes individual perspectives on sustainability, their differences, and similarities. This research also employs focus group discussions and interviews. In three regions in Indonesia.

Findings: Q-method resulted in two perspectives. The technologists perceive sustainable agriculture as food security and the use of organic pesticides. They also believe that the responsibility for sustainable agriculture lies with extension workers and governments. The environmentalists believe the concept of sustainability implies the active prevention of environmental degradation. They also believe that everybody should take responsibility for sustainability. The paper determines that boundary work needs highly motivated extension workers; the ability to gain trust from farmers; and government support.

Theoretical implication: This paper contributes to the literature on boundary work by connecting the concept of boundary work to agricultural extension.

Practical implication: The results may be used as inputs for Indonesian policymakers to develop a guideline on sustainable agriculture for extension workers.

Originality/value: In current studies on extension workers in developing countries, an analytical framework which employs the concept of boundary work is hardly found. Boundary work is a relevant concept to depict challenges extension workers are confronted with when brokering. Q-methodology aims to obtain individual perspective on a particular issue. This research provides insight on individual perspectives of extension workers on sustainable agriculture.     

Languages of origin and education in Europe: A systematic review

European education policies have tended to cultivate linguistic hegemony and preserve cultural uniformity, which has made it difficult for schools to support different cultural backgrounds. This has contributed to the promotion of artificial monolingual interactions that reinforce linguistic decapitalisation processes. The aim of the review on which this article reports was to investigate the treatment and education of languages of origin in Europe. We present findings from a systematic review of scientific articles published during the period 2010–2020. The main findings indicate that language and nationality are equated in European discourses and that public policies are promoted accordingly. These articulations draw on models that alternate between assimilationism and multiculturalism. Both assimilationist and multiculturalist approaches are detrimental to the supposed objective: that of promoting multilingualism. Thus, it is urgent that a consensus be reached, and certain public policies and educational practices promoted which are respectful of linguistic diversity, since the future social cohesion of our multicultural societies depends on this.     

Developing knowledge intensive ideas in engineering education: the application of camp methodology

Background: Globalization, technological advancement, environmental problems, etc. challenge organizations not just to consider cost-effectiveness, but also to develop new ideas in order to build competitive advantages. Hence, methods to deliberately enhance creativity and facilitate its processes of development must also play a central role in engineering education. However, so far the engineering education literature provides little attention to the important discussion of how to develop knowledge intensive ideas based on creativity methods and concepts.

Purpose: The purpose of this article is to investigate how to design creative camps from which knowledge intensive ideas can unfold.

Design/method/sample: A framework on integration of creativity and knowledge intensity is first developed, and then tested through the planning, execution and evaluation of a specialized creativity camp with focus on supply chain management. Detailed documentation of the learning processes of the participating 49 engineering and business students is developed through repeated interviews during the process as well as a survey.

Results: The research illustrates the process of development of ideas, and how the participants through interdisciplinary collaboration, cognitive flexibility and joint ownership develop highly innovative and knowledge-intensive ideas, with direct relevance for the four companies whose problems they address.

Conclusions: The article demonstrates how the creativity camp methodology holds the potential of combining advanced academic knowledge and creativity, to produce knowledge intensive ideas, when the design is based on ideas of experiential learning as well as creativity principles. This makes the method a highly relevant learning approach for engineering students in the search for skills to both develop and implement innovative ideas.     

On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro

The in vitro study of liver functions and liver cell specific responses to external stimuli deals with the problem to preserve the in vivo functions of primary hepatocytes. In this study, we used the biochip OrganoPlate^TM (MIMETAS) that combines different advantages for the cultivation of hepatocytes in vitro: (1) the perfusion flow is achieved without a pump allowing easy handling and placement in the incubator; (2) the phaseguides allow plating of matrix-embedded cells in lanes adjacent to the perfusion flow without physical barrier; and (3) the matrix-embedding ensures indirect contact of the cells to the flow. In order to evaluate the applicability of this biochip for the study of hepatocyte''s functions, Matrigel^TM-embedded HepG2 cells were cultured over three weeks in this biochip and compared to a static Matrigel culture (3D) and a monolayer culture (2D). Chip-cultured cells grew in spheroid-like structures and were characterized by the formation of bile canaliculi and a high viability over 14 days. Hepatocyte-specific physiology was achieved as determined by an increase in albumin production. Improved detoxification metabolism was demonstrated by strongly increased cytochrome P450 activity and urea production. Additionally, chip-cultured cells displayed increased sensitivity to acetaminophen. Altogether, the OrganoPlate seems to be a very useful alternative for the cultivation of hepatocytes, as their behavior was strongly improved over 2D and static 3D cultures and the results were largely comparable and partly superior to the previous reports on biochip-cultured hepatocytes. As for the low technical needs, this platform has the appearance of being highly applicable for further studies of hepatocytes'' responses to external stimuli.