Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design

研究目的：优化获得血红密孔菌（P.sanguineus）的最佳培养基组成,提高耐热漆酶的产量。创新要点：获得了目前文献报道的最高水平的漆酶活力。研究方法：通过单因素试验研究了不同培养基（番茄汁、麦麸、麦芽提取物和葡萄糖细菌蛋白胨培养基）和不同组合诱导剂（大豆油、阿魏酸、没食子酸、二甲基苯胺、酸性蓝62和活性蓝19分别与硫酸铜组合诱导剂）对P.sanguineus产耐热漆酶的影响。在此基础上采用中心组合试验设计,进一步研究了番茄汁培养基结合硫酸铜和大豆油组合诱导剂对P.sanguineus产耐热漆酶的影响。利用SAS10.0和响应面分析方法对试验结果进行了统计分析和建立回归模型。重要结论：通过中心组合设计优化得出P.sanguineus产耐热漆酶的最优培养基条件：以36.8%番茄汁为培养基,以3 mmol/L硫酸铜和1%大豆油作为组合诱导剂。该条件下在10 L搅拌槽生物反应器中漆酶活力达到了143 000 IU/L（2,2＇-联氮双（3-乙基苯并噻唑啉-6-磺酸）为底物,pH值为3.0）。     

Basic variables in reflection-impulsivity: A training programme to increase reflectivity

We found that reflective children in our study had better academic qualifications than impulsive ones. We worked with a sample of 201 Spanish children in their last year of Primary School. We elaborated and applied an intervention programme to increase reflectivity. The results were excellent and showed consistency and stability, which is very unusual in the context of R-I research. There was no significant difference between boys and girls’ performance on MFF20. The children were 12, 13 and 14 year olds and we found a reflection increase at 13 but not at 14. Contrary to the opinion of Salkind and Nelson on this point concerning MFFT, we believe that MFF20 is adequate for these ages. The Spanish children in our study were more reflective than the children from North-America (Salkind’s data) and Northern Ireland (Cairns and Cammock’s data). Basic scores of R-I had a higher stability and consistency with MFF20 than the ones from Kagan’s MFF test. I and E scores are adequate and there is no confusion between the slow-inaccurate and fast-accurrate children as stated by Gjerde, Block, and Block.     

On Defining and Developing Literacy across Communities

The paper attempts to raise a few issues relating to the task of defining and operationalizing literacy and literacy development across communities with diverse socio-economic profiles. In particular, the following concerns are raised: (1) Can literacy development be assessed using one set of domains, given that the domains of literacy practices vary across cultures? (2) Does the acquisition of literacy skills mean the same thing to people across different communities? (3) Does illiteracy have the same consequences for people in different communities? (4) Is the process of literacy acquisition and development the same across diverse communities? The paper underscores the importance of considering the diverse socio-economic patterns in different communities in trying to determine present levels of literacy development and in proposing programs to increase levels of literacy skill. It also proposes using the level of community (in addition to the macro-level of nation-state and the micro-level of individual) in analyzing matters relating to literacy development.     

Learning strategies. A training programme

We elaborated and applied a training programme to teach learning strategies to students in continuing adult education courses in Valencia (Spain) throughout an academic year. We worked with one control group and with one experimental group. These groups were equivalent in terms of pre-test assessments and there was not any significant difference of means either in learning strategies or in marks in Spanish and Maths. We found significant difference of means in the post-test, after the training programme, in learning strategies, and also an important difference in marks. Experimental students improved their learning strategies significantly along with their marks in Spanish and Maths, whereas control students didn’t improve either their learning strategies or their marks. So we obtained generalization and transference of results. We think that these results are important and that they confirm the effectiveness of the methods of the educational intervention which we used. This intervention is placed in the field of learning to learn.     

重新思考经济学与伦理学的联系

作为一门学科而言,经济学从诞生之初就与伦理学关系密切.古典经济学家如亚当·斯密、大卫·李嘉图、斯图尔特·米尔斯等,他们有的认为经济学是一门伦理学科,有的非常关注经济政策的伦理效应和社会行动者的社会责任.近年来世界经济危机之所以层出不穷,一个关键原因就在于经济学与伦理学的脱节,这些脱节表现在经济要素和伦理要素,经济观念、行为和伦理观念、经济规则和伦理规则等方面.复兴全球经济伦理学的呼声不绝于耳.我们需要将企业社会责任、公共政策的伦理责任、企业经理的伦理培训、社会资本等纳入一国经济社会发展的根本性议题之中.本文分析了经济学需要更多伦理要素的原因,厘清了当前世界经济面临危机时伦理问题的凸显,并提出了重新思考经济学与伦理学的大纲.     

Family violence exposure and associated risk factors for child PTSD in a Mexican sample

This study was undertaken in an effort to help illuminate the deleterious effects of traumatic stress on children and families in Mexico. Rates of exposure to traumatic events, family and community violence, and posttraumatic stress disorder (PTSD) were investigated in 87 school-age children and their mothers. Binary logistic regression analysis was performed to examine potential family and ecological risk factors for the presence of child PTSD. A total of 51 children (58.6%) reported an event that met the DSM-IV A criteria, and 36 children (41.4%; 20 boys and 16 girls) met criteria for full PTSD. Traumatic exposure in this sample was considerable, particularly intense, and chronic as a result of interpersonal violence in the home and community. Results support the need for preventive systemic interventions targeting the individual level, parent–child dyadic level, and the larger cultural and community context.     

Celebrating Difference: Best Practices in Culturally Responsive Teaching Online

Culturally responsive teaching and design practices flip the online classroom by creating an environment that acknowledges, celebrates, and builds upon the cultural capital that learners and teachers bring to the online classroom. Challenges exist in all phases of online course design, including the ability to create online courses that reflect the instructor’s commitment to inclusive excellence, diversity, and social justice. Designing an online environment that supports all learners regardless of their backgrounds is important in their future success as professionals; thus, it is important for faculty to design courses with all students in mind. The purpose of this article is to share best practices in the design of culturally and linguistically responsive online courses that support the culturally and linguistically diverse (CLD) students we serve. Based on Gay’s (2010) culturally responsive teaching practices, this article provides examples of online activities that are validating; comprehensive; multi-dimensional; empowering; transformative, and emancipatory.     

E-Mail as Spectroscopy: Automated Discovery of Community Structure within Organizations

We describe a method for the automatic identification of communities of practice from e-mail logs within an organization. We use a betweenness centrality algorithm that can rapidly find communities within a graph representing information flows. We apply this algorithm to an initial e-mail corpus of nearly 1 million messages collected over a 2-month span, and show that the method is effective at identifying true communities, both formal and informal, within these scale-free graphs. This approach also enables the identification of leadership roles within the communities. These studies are complemented by a qualitative evaluation of the results in the field.