Reasoning about data in middle school science

This case study illustrates instruction in an urban 6th‐grade classroom in which students were learning about mass, volume, and density by attempting to layer (stack) three miscible solutions with differing densities atop one another. The study examines classroom discourse and interaction on the basis of four teaching goals: (a) reaching consensus about which stacks were possible, (b) developing persuasive arguments that separated data from noise, (c) establishing social norms for collective inquiry, and (d) appreciating the epistemological status of scientific knowledge. The study traces the fate of three stacks that students claimed were possible after initial investigations with the solutions. These claims underwent a process of collective validation in which consensus without coercion was the goal, which illustrates emergent standards for backing claims with evidence, as well as for replicability, among the students. Students were successful in achieving three of the four goals, with some qualifications. In relation to Goal 3, which required generalization to other situations, somewhat less success is reported. Limitations in the current standards, difficulties of time allotment in current curricula, and establishing classroom cultures of inquiry are discussed. © 1999 John Wiley & Sons, Inc. J Res Sci Teach 36: 179–199, 1999     

Sub-nanomolar detection of thrombin activity on a microfluidic chip

Bioluminescence resonance energy transfer (BRET) is a form of Förster resonance energy transfer. BRET has been shown to support lower limits of detection than fluorescence resonance energy transfer (FRET) but, unlike FRET, has not been widely implemented on microfluidic devices for bioanalytical sensing. We recently reported a microscope-based microfluidic system for BRET-based biosensing, using a hybrid, high quantum-efficiency, form of BRET chemistry. This paper reports the first optical fiber-based system for BRET detection on a microfluidic chip, capable of quantifying photon emissions from the low quantum-efficiency BRET² system. We investigated the effects of varying core diameter and numerical aperture of optical fibers, as well as varying microfluidic channel design and measurement conditions. We optimized the set-up in order to maximize photon counts and minimize the response time. The optimized conditions supported measurement of thrombin activity, with a limit of detection of 20 pM, which is lower than the microscope-based system and more than 20 times lower than concentrations reported to occur in plasma clots.     

The effect of ball carrying method on sprint speed in rugby union football players

Different methods of ball carrying can be used when a player runs with the ball in rugby union. We examined how three methods of ball carrying influenced sprinting speed: using both hands, under the left arm and under the right arm. These methods were compared with running without the ball. Our aim was to determine which method of ball carrying optimizes sprinting speed. Altogether, 48 rugby union players (age 21 +/- 2 years, height 1.83 +/- 0.1 m, body mass 85.3 +/- 12 kg, body fat 14 +/- 5%; mean +/- s) were recruited. The players performed twelve 30-m sprints in total (each player performed three trials under each of three methods of carrying the ball and sprinting without the ball). The design of the study was a form of Latin rectangle, balanced across the trial order for each of the methods and for pairwise combinations of the methods in blocks of four per trial. Each sprint consisted of a 10-m rolling start, followed by a 20-m timed section using electronic timing gates. Compared with sprinting 20 m without the ball (2.58 +/- 0.16 s), using both hands (2.62 +/- 0.16 s) led to a significantly slower time (P < 0.05). Sprinting 20 m with the ball under the left arm (2.61 +/- 0.15 s) or under the right arm (2.60 +/- 0.17 s) was significantly quicker than when using 'both hands' (P < 0.05), and both these methods were significantly slower than when running without the ball (P < 0.05). Accordingly, running with the ball in both hands led to the greatest decrement in sprinting performance, although carrying the ball under one arm also reduced the players' sprinting ability. Our results indicate that to gain a speed advantage players should carry the ball under one arm.     

Resilience of anthropometric data assembly strategies to imposed error

The aim of this study was to examine strategies for selecting a criterion value during anthropometric data assembly and their resilience to imposed error. Sixty-seven women aged 16-60 years were subjected to three separate series of measurements, which included six skinfolds and three girths. A random error term was added to the first of the three series of measurements to produce a pseudo-series containing error, termed the 'erroneous replicated series'. Five strategies were then used to determine the criterion value of each of the replication series: the first measurement, the mean of the first two measurements, the mean of all three measurements, the mean of the closest two measurements and the median of the three measurements. The technical error between the criterion values of the true and the flawed replication series for each of the selection strategies was calculated. We found that, for five of the skinfolds and all of the girths, the median value provided the smallest technical error of measurement between the criterion values for the true and erroneous replication series. We conclude that the strategy of selecting the median of three measurements is the most resilient to imposed error.