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Carolyn W. Kohler 《Government Information Quarterly》1984,1(1):101-102
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Dmitriy Khodakov Leigh Thredgold Claire E. Lenehan Gunther G. Andersson Hilton Kobus Amanda V. Ellis 《Biomicrofluidics》2012,6(2)
Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional “tag sequence” linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes. 相似文献
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Given projected increases in workplace diversity, an understanding of diversity’s intersection with mentoring is a critical topic in the literature. This article involved an interview with Belle Rose Ragins, one of the world’s leading thinkers on diversity and mentoring in the workplace. After providing an overview of Ragins’ key achievements and contributions to the mentoring literature, the article delved into the interview, which involves four parts: (a) Ragins’ education and early influences; (b) her views on the extant literature as well as insights around future areas for inquiry; (c) her current research interests; and (d) her views on consulting and teaching. 相似文献
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This pilot study examined the effects of a short‐term music therapy program on the classroom behaviours of newly arrived refugee students who were attending an intensive ‘English as a Second Language’ secondary school. A cross‐over design with two five‐week intervention periods was employed with group music therapy sessions conducted one or two times per week. Data from the Behaviour Assessment Scale for Children were used to evaluate a range of positive and negative school behaviours. A significant decrease in externalising behaviours was found with particular reference to hyperactivity and aggression. No significant differences were found in other behaviours. Explanations and implications of these findings are discussed. 相似文献
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Abstract In this article, we investigate the use of multimedia information and communication technologies (ICTs) in integrated arts projects in two secondary schools. The ways in which teachers and students make sense of the technologies in relation to the dominant discourses of education/technology policy and educational research are explored before considering the implications for curriculum and teacher development. The ICT in the word contrad(ICT)ions is emphasised to represent the ways in which teachers and students' use of multimedia exposes and makes visible problematic issues in relation to literacy, school subjects and pedagogy. 相似文献
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Carolyn Skinner 《Technical Communication Quarterly》2013,22(4):307-324
This article examines 3 papers presented before the Cincinnati Academy of Medicine by 19th-century physician Julia W. Carpenter. The article identifies 3 strategies Carpenter used to negotiate the incompatible rhetorical expectations for women and for physicians. The published records of academy discussions provide evidence for Carpenter's colleagues' reactions to each strategy, revealing the complexity of her rhetorical situation and demonstrating the complex links among rhetorical practice, professional identity, and a communicator's social position. 相似文献