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排序方式: 共有193条查询结果,搜索用时 15 毫秒
11.
M. V. R. Reddy R. Alli B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2000,15(1):127-135
Lymphatic filariasis caused mainly by infection fromWuchereria bancrofti andBrugia malayi remains as the major cause of clinical morbidity in tropical and subtropical countries. Development of vaccine against filarial infection can act as additional measure to the existing therapeutic and vector control methods in the control of this disease. The main hurdles in the development of anti-filarial vaccine are the strict primate specificity ofWuchereria bancrofti, the paucity of parasite material, the diversity of clinical manifestations and their associated complex immune responses, lack of clear understanding on host-parasite interactions and the mechanisms involved in protective immunity. However in the past few years, the information generated in immuno-epidemiological studies, correlated with observations in experimental animals suggests that a filarial vaccine is feasible. Initially live irradiated infective larvae have been successfully used to induce high level of protective immunity in several animal models. Applying diverse strategies, variety of purified or recombinant filarial antigens have been explored for their ability to induce protection in different host-parasite systems. Some of these targeted filarial antigens induced high level of resistance in experimental animals against challenge infections. More focussed studies on thorough characterization of parasitological and immunological changes associated with resistance induced by such candidate protective antigens and on delivery mechanisms and safety aspects will be crucial in their selection for possible use in humans. 相似文献
12.
G. Kusumanjali Chinnapu G. Reddy A. S. Kanagasabapathy Pragna Rao 《Indian journal of clinical biochemistry : IJCB》2005,20(2):146-149
Accurate monitoring of blood cyclosporin C2 levels is vital to prevent over immunosuppression and acute renal toxicity in
patients who receive organ transplant. The matrix used to dilute patients’ C2 samples prior to the assay affected the final
measured values. Hence there was a need to develop a method of dilution that would accurately estimate C2 levels when cyclosporin
levels were beyond the calibration range of the method employed. Whole blood, cyclosporin free hemolysate and cell and protein
free supernatant obtained after pretreatment of normal blood were used to dilute patients’ C2 samples. C2 was measured in
188 patients using the supernatant method of dilution. C2 was correlated with Co and dose of cyclosporin received by the patient.
The use of cell and protein free supernatant obtained after pretreatment of normal blood as a C2 diluent detected higher levels
of C2 in the sample. Measured C2 correlated significantly with Co and the cyclosporin dose received by the patient. The uniformly
aqueous cell and protein free supernatant ensures uniform dilution of the patients’ C2 sample and measures higher cyclosporin
levels. 相似文献
13.
A. K. Pradhan A. K. Shukla M. V. R. Reddy N. Garg 《Indian journal of clinical biochemistry : IJCB》2004,19(1):83-87
Oxidative stress was assesed by estimating lipid peroxidation product (LPO) in the form of thiobarbituric acid reactive substances
(TBARS), enzymatic antioxidants in the form of superoxide dismutase (SOD), catalase and nonenzymatic antioxidant vitamins
e.g. vitamin C, β carotene and vitamin E in either serum or plasma or erythrocytes in 190 cases of age related cataract in
the age group of 50–80 years. 190 cases were grouped into three morphological types namely, 73 cases of cortical, 77 cases
of posterior subcapsular and 40 cases of nuclear cataract and values of LPO and antioxidants were compared with 78 cases of
age matched healthy control groups. Plasma TBARS levels were cataract cases when compared with control groups. There were
no significant differences in the erythrocyte levels of catalase and plasma levels of Vit E between cataract cases and control
groups. No significant changes of parameters were seen among three different morphological types of age related cataract.
The present study shows that the oxidative stress may play an important role in the age related cataract. 相似文献
14.
Donna Kalmbach Phillips Robert Ch. Nava 《Discourse: Studies in the Cultural Politics of Education》2011,32(1):71-83
This inquiry explores who is the ‘good teacher of color’. Through Michel Foucault's notion of biopower and disciplinary power, the analysis attempts to problematize the subject-position of ‘teacher of color’ by exploring how a Latino intern and a Latina intern negotiated their subjectivity in a large diverse school district. Participants' construction of subjectivity was found to be located within the nexus of the school district, the university, and the Latino counter-discourse through which biopower and disciplinary power operated. 相似文献
15.
In this paper we present a new fabrication method that combines for the first time popular SU-8 technology and PerMX dry-photoresist lamination for the manufacturing of high aspect ratio three-dimensional multi-level microfluidic networks. The potential of this approach, which further benefits from wafer-level manufacturing and accurate alignment of fluidic levels, is demonstrated by a highly integrated three-level microfluidic chip. The hereby achieved network complexity, including 24 fluidic vias and 16 crossing points of three individual microchannels on less than 13 mm(2) chip area, is unique for SU-8 based fluidic networks. We further report on excellent process compatibility between SU-8 and PerMX dry-photoresist which results in high interlayer adhesion strength. The tight pressure sealing of a fluidic channel (0.5 MPa for 1 h) is demonstrated for 150 μm narrow SU-8/PerMX bonding interfaces. 相似文献
16.
17.
P.V.K. Sasidhar P. Gopal Reddy 《The Journal of Agricultural Education and Extension》2013,19(4):387-407
Abstract Purpose: The purpose of this study was to identify and rank the SWOT issues of India's veterinary and animal science education. Design: The data were collected at the Indian Veterinary Research Institute (IVRI) from 168 post-graduate students. The two surveys generated 72% (N=121) and 68% (N=114) response rates, respectively. In the first stage, the vital SWOT issues were listed by respondents and in the second stage, they ranked the top eight issues on a Likert scale. Findings: Uniform curriculum; merit-based admissions; and huge demand and employment prospects were perceived as major strengths. Gaps in curriculum revision and inadequate faculty, colleges and hands-on experience were perceived as key weaknesses. Regular curriculum revisions; novel instructional methods; and single admission tests were important opportunities perceived. Out-dated education model; urban biased admissions; and regional barriers were the major threats perceived. Practical implication: The SWOT issues identified are similar to those faced by veterinary education programmes in many countries with minor variations. Originality/value: The findings are of interest for veterinary faculties in developing and under-developed countries to harmonize their veterinary education systems on par with global norms. The related implications for policy and future directions are discussed. 相似文献
18.
Minerva S. Chávez 《Equity & Excellence in Education》2013,46(2):334-348
This article investigates the role of autoethnographic research as the methodological tool of choice for a Chicana who positions herself along the liminal perspective. I posit that testimonios, autobiographical educational experiences, must be used as valid ethnographic research to contribute to existing knowledge around issues of educational equity. Producing autoethnographic research acknowledges and validates my Chicana presence as well as draws attention to my marginal position inside dominant structures of education. Autoethnography and critical race theory are the manners in which I think about the world and the ways I have chosen to engage in educational research. My work is derived from personal experience in Los Angeles urban schools and later in elite institutions of higher education. These distinct locations present a unique opportunity to problematize the internalized forms of class and racial structures that permeate educational institutions. Grounded in my own educational biography, testimonios frame my research perspective to interrogate the role that educational institutions play in the creation of particular ideologies in working-class students of color. 相似文献
19.
Sonika Gupta Niraj Shende Swati Banerjee Satish Kumar M. V. R. Reddy Bhaskar C. Harinath 《Indian journal of clinical biochemistry : IJCB》2002,17(1):5-8
Tuberculosis remains major health problem in India and developing countries Immunodiagnosis has important role in screening,
diagnosis and management of tuberculosis. SEVA TB ES-31 antigen has shown potential in detecting tuberculous IgG antibody
in earlier studies from our laboratory. In the present study we have analysedSEVA TB ES-31 antigen specific immunoglobulinsIgM, IgA and IgG in clinically and bacteriologically confirmed pulmonary tuberculosis cases to determine the usefulness of specific immunoglobulin
class in the diagnosis of patients attending the hospital.
Of the 30 cases of pulmonary tuberculosis 25 (83.3%) were positive for IgG, 19 (63.3%) for IgM and 16 (53.3%) for IgA. On
combining IgG and IgM positivity, sensitivity was increased to 93.3%. While combining IgG and IgA positivity, sensitivity
increased to 90%. However specificity was decreased to 66.6% and 70% for both of these combinations respectively. It could
be envisaged from this study that IgG antibody detection against ES-31 antigen showed acceptable sensitivity (83.3%) and specificity
(86.6%) compared to IgM or IgA alone or in combination. When immune responses were analysed according to degree of sputum
positivity, IgG response was observed to be predominant in all grades, compared to IgM or IgA antibody. The addition of IgM
or IgA as an adjunct test increases the sensitivity but at the cost of specificity. Hence the detection of IgG alone is more
useful compared to IgM or IgA assay, in detecting tuberculosis disease cases coming to the hospital. 相似文献
20.
Y. P. Bhandary K. N. Krithika Sandeep Kulkarni M. V. R. Reddy B. C. Harinath 《Indian journal of clinical biochemistry : IJCB》2006,21(1):1-7
Lymphatic filariasis caused mainly by infection fromW. bancrofti andB. malayi remains a major cause of clinical morbidity in tropical and subtropical countries. Analysis ofB. malayi mf, infective larval and adult worm lysates for the activity of enzymes led to the demonstration of activities of three key
enzymes of carbohydrate metabolism viz., Malate dehydrogenase (MDH), Malic enzyme (ME) and Glucose-6-phosphate dehydrogenase
(G6PDH) in all the three stages of the parasite. The specific activity of all the three dehydrogenases was significantly high
in mf lysate compared to their activity in lysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel
to their activity inlysates of the other two stages (P<0.001). Analysis by native polyacrylamide gel electrophoresis (PAGE)
using 7.5% non-gradient gel showed the presence of two isoforms of each of the three enzymes (MDH, ME & G6PDH) in mf lysate,
while only one form of each enzyme was present in L3 larval and adult worm lysates. Further proteolytic enzyme activity was demonstrated both in microfilarial and infective larval
lysates ofB. malayi. While both mf and L3 larval lysates showed optimal protease activity at alkaline pH of 9.0, the mf lysate showed increased activity also at pH
3.0. The infective larval lysate was markedly inhibited by Tosylamide-L-Phenylalanine chloromethyl ketone (TPCK), a thiol
protease inhibitor, while the protease activity in mf lysate was significantly inhibited by both TPCK and a serine protease
inhibitor Phenyl Methyl Sulphonyl Flouride (PMSF). In sodium do-decyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE),
using gelatin copolymerized gel, the microfilarial lysate showed 3 protease molecules of 40 kDa, 180 kDa and 200 kDa and the
L3 larval lysate had 6 protease molecules of 18, 25, 37, 49, 70 and 200 kDa size. 相似文献