A tapered channel microfluidic device for comprehensive cell adhesion analysis, using measurements of detachment kinetics and shear stress-dependent motion

We have developed a method for studying cellular adhesion by using a custom-designed microfluidic device with parallel non-connected tapered channels. The design enables investigation of cellular responses to a large range of shear stress (ratio of 25) with a single input flow-rate. For each shear stress, a large number of cells are analyzed (500–1500 cells), providing statistically relevant data within a single experiment. Besides adhesion strength measurements, the microsystem presented in this paper enables in-depth analysis of cell detachment kinetics by real-time videomicroscopy. It offers the possibility to analyze adhesion-associated processes, such as migration or cell shape change, within the same experiment. To show the versatility of our device, we examined quantitatively cell adhesion by analyzing kinetics, adhesive strength and migration behaviour or cell shape modifications of the unicellular model cell organism Dictyostelium discoideum at 21 °C and of the human breast cancer cell line MDA-MB-231 at 37 °C. For both cell types, we found that the threshold stresses, which are necessary to detach the cells, follow lognormal distributions, and that the detachment process follows first order kinetics. In addition, for particular conditions’ cells are found to exhibit similar adhesion threshold stresses, but very different detachment kinetics, revealing the importance of dynamics analysis to fully describe cell adhesion. With its rapid implementation and potential for parallel sample processing, such microsystem offers a highly controllable platform for exploring cell adhesion characteristics in a large set of environmental conditions and cell types, and could have wide applications across cell biology, tissue engineering, and cell screening.     

Higher education curriculum orientations and the implications for institutional curriculum change

This research is based on an empirical study exploring how academics make curriculum decisions and their perceptions of the influences that shape their decisions. Interviews were held with 20 academics from diverse disciplines, who were both research active and committed to teaching. The higher education curriculum was conceptualised as a field of decision-making shaped by academics’ beliefs about educational and contextual influences. The study identified five distinctive curriculum orientations representing coherent patterns of curriculum decisions aligned with academics’ beliefs about educational purposes. Case studies are presented to elucidate each of the curriculum orientations. Curriculum orientations were also found to shape academics’ responses to educational change. The following higher education change drivers are explored: graduate employability and the skills agenda, teaching–research relationships, changing understandings about teaching and learning, educational technologies and flexible delivery. The findings suggest implications for institutional curriculum change initiatives and academic development programmes.     

Androgyny and the Mentoring Role: an empirical study to examine for prominent mentor expectations

abstract

As part of an ongoing research design, we wished to examine how mentors behave whilst in the mentoring role. The starting point for this was a study to examine how mentors felt they were expected to behave in context; that is, an organised educational mentoring situation, as we recognised the importance of situation‐specificity. Prior to empirical study, we theorised that the mentor may be androgynous; or more precisely are able to display high levels of expressive and instrumental behaviour. Seven suppositions were examined. Our findings were that whilst in the role, mentors do indeed feel expected to blend and display high levels of both instrumentality and expressiveness. Further, the levels of expressive behaviour they perceived they were expected to display were significantly higher. The higher levels of expected expressive behaviour is not congruent with the androgyny literature: our samples proved an exception to the ‘masculine supremacy effect’. Also, that mentoring may require high levels of stereotypically feminine behaviour is something that the mass of mentoring literature has not discussed and may warrant further attention. We concluded that the diverse, complex and complicated nature of mentoring is met with expectations to display flexibility and versatility across situations — to take a proactive approach to coping with mentoring situational demands — such is not mitigated by gender. Their reported high expected levels of both expressive and instrumental behaviour are congruent with our theory of the androgynous mentor.     

Gender and Ethnicity Among Community College Students: The Relationship Between Adlerian Themes

This study explored the relationship between Adlerian themes, gender, and ethnicity. Results indicate significant differences between Hispanic and Caucasian (non-Hispanic) community college students in Belonging and Social Interest as measured by the BASIS-A. Differences between males and females in Going Along and Wanting Recognition are also explored. The findings are discussed in relation to college students, teaching, and classroom climates.     

NURTURING EARLY CHILDHOOD PROFESSIONALISM: THE RHODE ISLAND EARLY CHILDHOOD SUMMER INSTITUTE

This article describes the independent study project of a student who was a graduate assistant in a child development laboratory preschool when Hurricanes Katrina and Rita struck the Gulf Coast area. Through her experiences with “Project Katrina” she deepens her understanding of developmentally appropriate practices as she learns firsthand about the project approach and writing an article for publication in a professional journal.     

An Instructional Strategy to Introduce Pedagogical Content Knowledge Using Venn Diagrams

This paper describes the use of a three-circle Venn diagram as a vehicle for introducing pre-service elementary teachers to pedagogical content knowledge (PCK). Each circle of the diagram represents pedagogy, content and context individually. The overlap of any two circles represents the interaction between the circles. For example, the overlap of pedagogy and context relates to the ways that each of these general topics influences the other. The overlap of all three circles represents a complete lesson that is an integration of the three major components of PCK. The Venn diagram is an easily remembered graphic illustration of PCK that can be useful in planning lessons. The use of this graphic organizer in a science capstone course required of all pre-service elementary teachers is described.     

Goal orientation towards teaching (GOTT) scale

Goal orientation theory has been widely investigated and found to affect many motivation and behavior variables in relation to student learning and work performance. However, unlike the motivational construct of self-efficacy, researchers have yet to investigate whether this theory can be applied to the field of teaching and contribute to the explanation for trends in instructional behavior and motives. The purpose of the present study was to develop an instrument to begin the exploration of teachers’ goal orientation towards teaching. From a three-phase research design consisting of scale development, score validation, and convergent/discriminant validation, results provided support for the development of, and validity of scores on, a Goal Orientation towards Teaching (GOTT) Scale. Although further validation is needed, future researchers and practitioners can use the GOTT scale to investigate how teachers’ goal orientation towards teaching can impact student learning, teaching effectiveness, pedagogical learning, and even professional morale.     

马桶座圈——真的是你家里最脏的地方吗?

马桶座圈背负着一个不公平的污名,那就是被认为是一般家庭中最脏的物件。但科学家们称,我们的家里其实有更为污秽的地方,有些地方还是最令人意想不到的。你会在马桶座圈上切菜吗?我想基本上所有人都说不会。但或许我们得重新考虑一下了。     

Efficacy of using non-linear pedagogy to support attacking players’ individual learning objectives in elite-youth football: A randomised cross-over trial

ABSTRACT

The present study examined the efficacy of a coaching curriculum, based on non-linear pedagogy, on improving attacking players’ individual learning objectives (ILOs) in elite-youth football. Participants included 22 attacking players (i.e., centre-forwards, wide-players and attacking midfield players) from a professional football academy in England. The players were randomly appointed to both control (CON) and intervention (INT) periods following baseline measures. The INT (non-linear) and CON (linear) periods were both designed to support the ILOs provided to each player as part of the elite player performance plan. The study adopted a randomised cross-over design and ILOs considered important for attacking players (i.e., strong foot finishing, weak foot finishing, 1-v-1 and decision-making) were evaluated using the Loughborough Shooting Skill Test. The results showed significant differences for INT in 1-v-1 (P< 0.02) and decision-making (P< 0.01). However, there were no significant differences for strong foot finishing, weak foot finishing or time taken. These results support non-linear pedagogy in developing 1-v-1 game play and decision-making but not for technical shooting proficiency.     

Reading Instruction for English Learners in the Middle Grades: a Meta-Analysis

This meta-analysis synthesizes the last two decades of experimental and quasi-experimental research on reading instruction across academic contexts (e.g., social studies, science, mathematics, English language arts) for English learners (ELs) in grades 4 through 8, to determine (a) the overall effectiveness of reading instruction for upper elementary and middle school students who are ELs and (b) how the magnitude of the effect varies based on student, instructional, and study characteristics. The analysis included a total of 11 studies with 46 individual effect sizes and yielded a mean effect size of g?=?0.35 across all (i.e., standardized and unstandardized) reading measures, g?=?0.01 across standardized reading measures, and g?=?0.43 across unstandardized reading measures. For all reading, unstandardized reading, all vocabulary, and unstandardized vocabulary measures, results suggest that higher quality studies tended to have smaller effects, and these effects were even more evident for unstandardized measures (i.e., one unit increase in study quality was associated with decreased effects: g?=?0.21, g?=?0.30, g?=?0.24, g?=?0.30, respectively). For all comprehension measures, effects were larger for instruction that included both vocabulary and comprehension (g?=?0.39) than for instruction that focused on vocabulary alone (g?=?0.08). Results suggest the benefit of developing and refining high-impact approaches to reading instruction for ELs that can be delivered across content areas and grades.