发达国家高职教育实训基地建设的比较研究

发达国家高等职业技术教育发展迅速,其成功经验值得借鉴。本文介绍和分析了发达国家高职教育实训基地建设做法和经验,提出了我国高职教育实训基地建设应采取的方法和措施。     

An integrated microfluidic system for screening of phage-displayed peptides specific to colon cancer cells and colon cancer stem cells

Affinity reagents recognizing biomarkers specifically are essential components of clinical diagnostics and target therapeutics. However, conventional methods for screening of these reagents often have drawbacks such as large reagent consumption, the labor-intensive or time-consuming procedures, and the involvement of bulky or expensive equipment. Alternatively, microfluidic platforms could potentially automate the screening process within a shorter period of time and reduce reagent and sample consumption dramatically. It has been demonstrated recently that a subpopulation of tumor cells known as cancer stem cells possess high drug resistance and proliferation potential and are regarded as the main cause of metastasis. Therefore, a peptide that recognizes cancer stem cells and differentiates them from other cancer cells will be extremely useful in early diagnosis and target therapy. This study utilized M13 phage display technology to identify peptides that bind, respectively, to colon cancer cells and colon cancer stem cells using an integrated microfluidic system. In addition to positive selection, a negative selection process was integrated on the chip to achieve the selection of peptides of high affinity and specificity. We successfully screened three peptides specific to colon cancer cells and colon cancer stem cells, namely, HOLC-1, HOLC-2, and COLC-1, respectively, and their specificity was measured by the capture rate between target, control, and other cell lines. The capture rates are 43.40 ± 7.23%, 45.16 ± 7.12%, and 49.79 ± 5.34% for colon cancer cells and colon cancer stem cells, respectively, showing a higher specificity on target cells than on control and other cell lines. The developed technique may be promising for early diagnosis of cancer cells and target therapeutics.     

党风廉政建设视域下举报人安全机制的构建

举报对加强党风廉政建设具有非常重要的作用。然而,现在的举报制度和保护措施不健全,造成举报人屡受伤害,扼杀了举报的积极性。鉴于此,国家应该加强机制建设,制定专门的法律保护举报人,实行泄露举报人信息问责制,建立中央直管的举报信息系统,完善举报人救助制度,发挥媒体的舆论引导作用,让举报成为人民监督的重要手段。     

低压配电系统无功补偿柜的设计

无功功率补偿将降低供电系统的功率损耗和电能损耗,减少变压器和线路中的电压损失和提高供电设备利用率。试通过鸡西赫阳燃气有限公司低压配电系统无功补偿柜的设计过程来阐述低压配电系统无功补偿方式选择及无功补偿容量的确定。     

“双减”政策背景下青少年体育活动开展策略研究

为了在“双减”政策背景下进一步推进青少年体育活动的开展,通过文献资料法、综合分析法等研究方法,分析了目前青少年体育活动面临的一些问题,并提出如下对策:树立正确的体育价值观,强化体育意识的培养;扩大资源的有效供给,推动青少年体育活动的开展;发挥“家、校、社”主导作用,切实建设联动共同体;建立长效保障机制和治理监管体系,有效防范化解各类风险。     

“九五”基金重大项目“材料在我国自然环境中腐蚀数据积累及规律性研究”取得重要进展

持续积累材料在我国典型自然环境中腐蚀数据8.6万多个。开发了海 水腐蚀形貌诊断系统；建立了腐蚀电化学现场原位检测新方法；发展了腐蚀数据处理方法， 建立了腐蚀性的评价模型；完善了我国材料环境腐蚀数据总库；完成了西部地区腐蚀调查。 材料环境腐蚀数据和研究成果在国家重大工程建设中得到应用。     

知识考古与历史重诂:编撰《林纾年谱长编》的构想

论述编撰《林纾年谱长编》的总体构想。课题尝试运用现代史学方法对近代闽籍历史名人林纾进行知识考古和重诂,涉及谱主一生四个不同特质的时期。介绍这项课题的选题背景、动机和主要任务,同时提出年谱编撰方法方面的新观点。研究的目的是以新的历史观重构特定时期的历史人物。     

《诗经》单音节形容词词义的发展变化浅析

《诗经》中单音节形容词数量多、使用频繁且极富表现力,但历来对它的研究甚少。由于社会的发展、语言的演变以及人们认识水平的提高等诸多因素的影响,这些单音节形容词的词义也发生了很多变化。本文对单音节形容词词义的古今发展变化情况做了较为详细的阐述,力求使读者在欣赏诗歌的同时,注意词义的古今变化,加深对诗歌内容的理解。