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51.
Dmitriy Khodakov Leigh Thredgold Claire E. Lenehan Gunther G. Andersson Hilton Kobus Amanda V. Ellis 《Biomicrofluidics》2012,6(2)
Herein, we describe the development of a novel primer system that allows for the capture of double-stranded polymerase chain reaction (PCR) amplification products onto a microfluidic channel without any preliminary purification stages. We show that specially designed PCR primers consisting of the main primer sequence and an additional “tag sequence” linked through a poly(ethylene glycol) molecule can be used to generate ds-PCR amplification products tailed with ss-oligonucleotides of two forensically relevant genes (amelogenin and human c-fms (macrophage colony-stimulating factor) proto-oncogene for the CSF-1 receptor (CSF1PO). Furthermore, with a view to enriching and eluting the ds-PCR products of amplification on a capillary electrophoretic-based microfluidic device we describe the capture of the target ds-PCR products onto poly(dimethylsiloxane) microchannels modified with ss-oligonucleotide capture probes. 相似文献
52.
This paper demonstrates how the application of New Public Management (NPM) and the accompanying rise of academic capitalism
in allocating research funds in the German academic field have interacted with a change from federal pluralism to a more stratified
system of universities and departments. From this change, a tendency to build cartel-like structures of allocating symbolic
capital resulting in oligopolistic structures of appropriating research funds has emerged. This macro level structure is complemented
by the strengthening of the traditional oligarchic structures of research, carried out by an increasing number of assistants
under the direction of a professor on the meso level. The outcome of this institutional setting is a significant gap between
the appropriation of research funds according to the allocation of symbolic capital and the production of knowledge in publications.
The application of NPM therefore needs to be explained more as a result of the normative pressure of a globally established
model of “rational” administration, and less as a result of its functional effectiveness. This is demonstrated by an empirical
analysis with simple and multiple regressions using data on the allocation of research grants and publication records of German
chemistry departments. 相似文献
53.
Christian Rittelmeyer 《Zeitschrift für Erziehungswissenschaft》2013,16(3):217-231
Transfer research examines how artistic activities can have an impact outside of the realm of art itself. The article concludes that this research can be carried out in a meaningful way, only within the framework of a comprehensive theory of aesthetic education. This theory would encompass the structural analysis of artistic activity, as well as biographical reports about artistic experiences. The elucidation of the concepts or themes relevant to the theory would include the notion of aesthetic experience, the understanding of immediate, aesthetic experiences, the analysis of artistic processes, and the outcomes of scientifically evaluated applications of aesthetic education. 相似文献
54.
55.
Christian Vater Andrew Mark Williams Ernst-Joachim Hossner 《International review of sport and exercise psychology》2020,13(1):81-103
ABSTRACT The role and importance of peripheral vision have been discussed across various sports. Yet, its functionality remains unclear and the terms employed in the literature to characterize the use of peripheral vision are not well defined. We present a review of 29 studies focusing on the use of peripheral vision in sports. We focus on the methodological approaches employed to study its use and identify the mechanisms that may underlie the effective use of peripheral vision in sport. We define key differences between ‘gaze anchors’ and ‘visual pivots’ and introduce the concept of a ‘foveal spot’. All three gaze behaviors, while being characterized by a consistent, dynamically adjustable gaze location, have different functionalities. A gaze anchor is a cue-optimized position used for the monitoring of peripheral cues and avoiding the negative consequences of saccades (i.e. information suppression), especially under time pressure. A visual pivot is a distance-optimized location between relevant cues which allows athletes to optimally initiate saccades to those cues, especially if the costs of eye-movements are low. A foveal spot is primarily used for information processing via the fovea. Finally, we highlight suggestions for future research to improve our understanding of the functional differences between these gaze behaviors. 相似文献
56.
57.
Colleen Stieler-Hunt Christian Jones 《British journal of educational technology : journal of the Council for Educational Technology》2019,50(1):264-279
The benefits of using digital games in the curriculum are well documented in literature. Most teachers who use digital games use short-form drill-and-practice learning games rather than the kinds of games most students would choose to play in their free time. The use of more interactive, immersive digital games (IDGs) in classrooms tends to be sporadic, dependent upon the enthusiasm and ingenuity of individual teachers. Previous studies have indicated that many teachers have concerns about using digital games in classrooms and have difficulties knowing how to best use digital games. This qualitative study presents a professional development model for teachers that was inductively derived through analysing in-depth, semi-structured interviews with 13 educators who have used digital games in the classroom. Implementing this model can help teachers develop attitudes and skills necessary to meaningfully use interactive, IDGs in their classrooms. Evidence suggests that a mentor should have a strong understanding of how to use digital games effectively in classrooms, work with small cohorts of teachers to collegially plan the use of digital games within a unit of class work, trial the unit of work with a small number of enthusiastic teachers, and then implement it with the remaining teachers. This approach is based on interviews with educators who have successfully mentored teachers in their schools to use interactive, IDGs in their classrooms. These educators encountered little resistance from their teaching colleagues. Their approach further supported teachers who were open to the possibilities of using digital games in their classrooms. 相似文献
58.
59.
Clara Ginther Karin Lackner Christian Kaier 《New Review of Academic Librarianship》2017,23(2-3):136-147
ABSTRACTEstablishing Publication Services in the library at the University of Graz did more than broaden the service portfolio of the library. A convergence of expertise at the library, needs of researchers at the university, and ongoing changes in scholarly communication also contributed to the evolution of the library's role and profile. The new services offer first-level support for matters pertaining to scholarly publishing and communications. Furthermore, Publication Services has developed into a knowledge sharing platform, extending beyond the library to other administrative departments and creating a community of practice. 相似文献
60.
Johannes S. Binder Michael Scholz Stephan Ellmann Michael Uder Robert Grützmann Georg F. Weber Christian Krautz 《Anatomical sciences education》2021,14(1):22-31
Integration of medical imaging into preclinical anatomy courses is already underway in many medical schools. However, interpretation of two-dimensional grayscale images is difficult and conventional volume rendering techniques provide only images of limited quality. In this regard, a more photorealistic visualization provided by Cinematic Rendering (CR) may be more suitable for anatomical education. A randomized, two-period crossover study was conducted from July to December 2018, at the University Hospital of Erlangen, Germany to compare CR and conventional computed tomography (CT) imaging for speed and comprehension of anatomy. Sixteen students were randomized into two assessment sequences. During each assessment period, participants had to answer 15 anatomy-related questions that were divided into three categories: parenchymal, musculoskeletal, and vascular anatomy. After a washout period of 14 days, assessments were crossed over to the respective second reconstruction technique. The mean interperiod differences for the time to answer differed significantly between the CR–CT sequence (−204.21 ± 156.0 seconds) and the CT–CR sequence (243.33 ± 113.83 seconds; P < 0.001). Overall time reduction by CR was 65.56%. Cinematic Rendering visualization of musculoskeletal and vascular anatomy was higher rated compared to CT visualization (P < 0.001 and P = 0.003), whereas CT visualization of parenchymal anatomy received a higher scoring than CR visualization (P < 0.001). No carryover effects were observed. A questionnaire revealed that students consider CR to be beneficial for medical education. These results suggest that CR has a potential to enhance knowledge acquisition and transfer from medical imaging data in medical education. 相似文献