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991.
Reginald Tran Byungwook Ahn David R. Myers Yongzhi Qiu Yumiko Sakurai Robert Moot Emma Mihevc H. Trent Spencer Christopher Doering Wilbur A. Lam 《Biomicrofluidics》2014,8(4)
Cell culture in microfluidic systems has primarily been conducted in devices comprised of polydimethylsiloxane (PDMS) or other elastomers. As polystyrene (PS) is the most characterized and commonly used substrate material for cell culture, microfluidic cell culture would ideally be conducted in PS-based microsystems that also enable tight control of perfusion and hydrodynamic conditions, which are especially important for culture of vascular cell types. Here, we report a simple method to prototype perfusable PS microfluidics for endothelial cell culture under flow that can be fabricated using standard lithography and wet laboratory equipment to enable stable perfusion at shear stresses up to 300 dyn/cm2 and pumping pressures up to 26 kPa for at least 100 h. This technique can also be extended to fabricate perfusable hybrid PS-PDMS microfluidics of which one application is for increased efficiency of viral transduction in non-adherent suspension cells by leveraging the high surface area to volume ratio of microfluidics and adhesion molecules that are optimized for PS substrates. These biologically compatible microfluidic devices can be made more accessible to biological-based laboratories through the outsourcing of lithography to various available microfluidic foundries. 相似文献
992.
993.
Christopher DeLuca 《Educational research; a review for teachers and all concerned with progress in education》2013,55(3):303-320
Background:?Validity theory has evolved significantly over the past 30 years in response to the increased use of assessments across scientific, social and educational settings. The overarching trajectory of this evolution reflects a shift from a purely quantitative, positivistic approach to a conception of validity reliant on the interpretation of multiple evidence sources integrated into validity arguments. Moreover, within contemporary validity, interpretation has been emphasised as a central process; however, despite this emphasis, there have been few explicit articulations of specific interpretive methodologies applicable to the practice of validation. Purpose:?To link contemporary theoretical foundations in validity to practical methods and structures to help guide the collection and analysis of interpretive validity evidence. By building upon existing validity theory, this paper aims to provide greater clarity on the practice of validation and contribute toward the larger developing framework for the validation of educational assessments. Source of evidence:?An interdisciplinary, integrative review of over 60 research articles and sources related to the theory and practice of educational validation and interpretive inquiry approaches. Sources include literature from the fields of educational assessment and more broadly social scientific research. Main argument:?As assessments in education increasingly aim to measure complex constructs that are value-laden and socially dependant, validity theory must keep pace and evolve in ways that address the inherent complexities associated with contemporary educational assessment. Through this paper, I assert that a greater understanding of interpretive methodologies represents one of the most promising areas for development of validation theory and practice. Specifically, I argue that dialectic, hermeneutic and transgressive forms of inquiry can be integrated within current argument-based structures for the collection, analysis and representation of validity evidence in several useful ways. Conclusions:?Interpretive inquiry processes, namely dialectic, hermeneutic and transgressive forms of interpretation, serve to expand validation practice to include diverse evidences for the generation of multiple-perspective validity arguments. The paper concludes with specific implications for future research and practice within the field of interpretive validity theory. 相似文献
994.
More universities are offering online instruction for students though we know little about effective online learning. Some have found online instruction increases student participation while others have reported that students prefer the traditional face‐to‐face format This study of gifted education graduate students follows the expectation that online students ought to have time to be more thoughtful with online course interactions as compared to the time‐constrained interactions in a face‐to‐face course. Researchers evaluated students’ thinking levels (as per Bloom's Taxonomy) in the online discussion forums required by a graduate course in gifted education. Results indicate there was no relationship between the level of the prompt and the level of the responses. Higher level prompts did not necessarily generate higher level responses. The research‐developed Rubric for Evaluation of Online Discussions can be used both as an instructional guide and as an evaluation rubric to assess the level of online discussions. 相似文献
995.
Character education has always played a role in the purpose of schools. Most US states have a statement about character education as a part of the mission of the schools. This research studied how character education was perceived by participants in regards to school mission statements/philosophies, school atmosphere and curriculum in a Catholic school, a Quaker school and a public school. Using interviews and observations, the public school and two private schools approaches to character education were explored. Character education explicitly connected and applied from the mission statement manifested more fully in the school culture. 相似文献
996.
Christopher Lubienski 《牛津教育评论》2013,39(4):498-513
The American experiment with charter schools advanced on dual impulses of increasing opportunities for disadvantaged students and unleashing market competition. While critics see these independently managed schools as a form of privatisation, proponents contend that they are public schools because of funding and accountability arrangements and potential benefits, and believe that the economic logic around these schools will produce equitable educational opportunities. This analysis considers how charters are or are not instances of privatisation in education, showing that the marketised environment they are intended to nurture serves as a route for profit-seeking strategies. In reviewing the research on charter school organisational behaviour and outcomes in marketised environments, I find evidence of de facto privatisation in function if not in form. As charter schools often act like profit-seeking entities, but fail to achieve expected academic and equity outcomes, the concluding discussion considers how these schools are placed between conflicting goals, and serve as entry points for private organisations seeking to penetrate the publicly funded education sector. I conclude that perhaps their most important role is in serving as a vehicle for privatising public policy—diminishing the public while enhancing the position and influence of private interests and organisations in education policymaking. 相似文献
997.
Appropriate implementation of information technology (IT) can help create a more efficient, less costly, and higher-quality service-delivery environment for community-based organizations that serve older people. Relevant studies and reports on technology in healthcare can be compared and applied to these organizations. This study is the result of an assessment and analysis of the technological needs of 17 community-based organizations that serve older people in Orange County, California. Preliminary results suggest that appropriate implementation of technology, along with complementary workflow processes and positive attitudes toward technology usage, might lead to improvements in the level and quality of service in these agencies. 相似文献
998.
999.
1000.
Research Findings: The National Association for the Education of Young Children's guidelines for developmentally appropriate practices (DAP) have been imported and researched by others across the globe. A central issue that has arisen for these international early childhood educators is whether these best practices are sensitive to the sociocultural contexts in which they work. To shed further light on this issue, a qualitative metasynthesis was conducted that examined findings from published peer-reviewed studies of DAP in international early childhood contexts that investigated how early educators conceptualized and/or engaged in practices with young children. It then looked at whether these understandings and/or practices culturally contrasted with DAP. In brief, matters involving culture that contrasted with the framework of DAP arose frequently for the early educators in the data set. Yet researchers rarely offered suggestions for early educators to attend to these issues. Practice or Policy: It seems that to address matters involving culture that contrast with the framework of DAP in international contexts early educators should examine the 3 central tenets of DAP simultaneously when addressing the needs of children—their sociocultural, individual, and developmental needs. It also appears that advocates for DAP need to consider whether particular materials, resources, and/or policies should be in place in any early childhood education context for early educators to conceptualize as well as practice DAP. Lastly, there is a need for further research that examines how teachers in international contexts are responding to cultural issues that contrast with DAP in their conceptions of and/or practices in early education. 相似文献