Serial composition of human milk in preterm and term mothers

Human milk samples were collected from 86 mothers induced in the study on days 3, 7, 14 and 28 of lactation. The mothers were divided into three groups on the basis of gestation viz. group 1: 37–41 weeks (n=41), group II: 33–36 (n=23) and group III: <33 weeks gestation (n=22). All the samples were analysed for the estimation of fat, lactose and protein. The results obtained from the investigations were statistically analysed. The analysis of the results revealed a lower amount of fat and lactose in preterm milk as compared to term milk (p<0.01). These were found to increase in amount with increasing postnatal age (p<0.05). The protein levels were observed to be significantly higher in preterm than term milk (p<0.01). These were observed to decrease significantly with increase in postnatal age (p<0.01).     

Polymorphism (C677T) in the 5,10-methylenetetrahydrofolate reductase (MTHFR) gene: A preliminary study on north Indian men

An elevated level of plasma homocysteine, sulfur containing amino acid generated through demethylation of methionine has been widely accepted as a risk factor for cardiovascular disease (CVD). The increase can result from genetic and/or nutrient related disturbances in the remethylation or transsulfuration pathways for homocysteine metabolism. A common mutation (C677T) in the gene encoding for the enzyme 5, 10-methylenetetrahydrofolate reductase (MTHFR) or deficiency of the B vitamins namely folic acid, B₁₂, B₆ can lead to hyperhomocysteinemia. In the present study, we have investigated the incidence of the (C677T) MTHFR polymorphism in the North Indian males. 141 angiographically proven coronary artery disease (CAD) patients and 55 age and sex matched healthy volunteers were examined for the association between MTHFR gene polymorphism and CAD. The MTHFR genotyping was performed using polymerase chain reaction (PCR) followed by restriction-isotyping with Hinf 1 endonuclease. A trend for higher ‘T’ allele frequency (0.19) was observed in patients than in controls (0.16). However no significant association was found between C677T mutation and CAD severity. The lack of statistical significance could be due to the small sample size studied. Hence a larger study including various ethnic groups is warranted.     

Learner Groups in Massive Open Online Courses

The open nature of Massive Open Online Courses (MOOCs) attracts a large number of learners with different backgrounds, skills, motivations, and goals. This has brought a need to understand such heterogeneity in populations of MOOC learners. Categorizing these learners based upon their interaction with the course can help address this need and suggest possible improvements in course design and delivery. In this article, the K-means clustering technique with careful seeding is used to obtain clusters of learners having similar interaction in the course. Learners are grouped based on their interaction with course material, video lectures, discussion forums, and assessments. In the analysis of thirteen courses, the proposed method identified learners’ classes as Uninterested, Casuals, Performers, Explorers and Achievers. Each class of learners had distinct interaction with the course and followed a certain learning approach. The learners’ classes were mapped to the standard surface, deep, and strategic learning approaches.This article also highlights the data preparation phase and its importance in data mining.     

An ensemble approach in converging contents of LMS and KMS

Currently the challenges in e-Learning are converging the learning content from various sources and managing them within e-learning practices. Data mining learning algorithms can be used and the contents can be converged based on the Metadata of the objects. Ensemble methods use multiple learning algorithms and it can be used to converge the Learning Objects from Learning Management Systems (LMS) and Knowledge Objects from Knowledge Management System (KMS). This can increase the performance of the learning system, especially when there is different content available from a variety of models. In this research, Data mining ensemble techniques are used so that an appropriate learning content is delivered to the learner. By converging, the learning content from various sources the Learning system pedagogies can also be revolutionized and a right learning path can be provided to the learners. This research work uses various classification techniques for converging and are evaluated using statistical measures.     

Study of apolipoproteinc3 Sstl polymorphism in healthy volunteers from Northern India

Several studies including a small case-control (hypertriglyceridemic/normotriglyceridemic individuals) study by us revealed close association between rare S2 allele ofAPOC3 Sstl polymorphism and hypertriglyceridemia. With the understanding that Asian Indians are highly vulnerable to the adverse effects of hypertriglyceridemia, we extended the investigation and studied the frequency distribution of this polymorphism in 216 healthy volunteers from Northern plains of India. We found that more than 50% of the study population had one or two S2 allele. This may suggest that a larger fraction of this population is genetically predisposed to hypertriglyceridemia.     

1, 25-Dihydroxycholecalciferol (1,25-(OH)<Subscript>2</Subscript> D<Subscript>3</Subscript>) levels in osteoporosis

In the present study primary involution osteoporosis and vitamin D levels were studied in 60 subjects including thirty controls. The biochemical analysis of serum calcium, phosphorus, ALP, albumin and vitamin D₃ (1,25-(OH)₂ D₃) levels were significantly decreased in osteoporotic patients when compared to non-osteoporotic control group. There was a significant correlation of magnitude of sun exposure with 1,25-(OH)₂ D₃ levels. Similarly, vitamin D intake in the diet has a significant correlation with 1,25-(OH)₂ D₃ concentration. However, no correlation could be obtained between vitamin D₃ levels with increasing age. Among the biochemical markers serum calcium, phosphorus, ALP and albumin could not be correlated with 1,25-(OH)₂ D₃ levels.     

Diagnostic potential of fractionatedMycobacterium tuberculosis H37Ra excretory-secretory (EST-DE1) antigen in pulmonary tuberculosis

Tuberculosis is emerging as a major public health problem in developing and developed world. Early and precise diagnosis is of prime importance in successful control of infection. Indirect ELISA with penicillinase as marker was developed using purifiedM. tuberculosis excretory-secretory (EST-DE₁) antigen for detecting IgG antibodies in pulmonary tuberculosis. The assay System gave a overall sensitivity of 82% for both smear positive and smear negative pulmonary tuberculosis cases with a specificity of 84%. The positive and negative predictive values were 75% and 88% respectivaly. Further studies with EST-DE₁ antigen revealed that, it contains two of the active antigen fractions of Mtb EST antigen i.e. Mtb EST-4 (56–68 KDa) and Mtb EST-6 (37–45 KDa), as demonstrated by inhibition ELISA. Reactivity with monoclonal antibodies HGT 3a showed the presence of 38 KDa molecule in EST-DE₁ antigen.     

Serum ionised calcium in birth asphyxia

Serum total and ionised calcium levels were measured at birth and at 48 hours in 25 term neonates with birth asphyxia (one minute APGAR score of 6 or less) and in 25 normal term neonates (one minute APGAR score of 7 or more). Infants were categorised into two groups TAGA (term appropriate for gestational age) and TSGA (term small for gestational age). Asphyxiated infants had significantly lower serum total and ionised calcium values at birth as well as at 48 hours. Abnormal clinical features were observed in 48% of asphyxiated infants. Low ionised calcium was detected in symptomatic babies, who had otherwise normal total calcium values. Due to hyocalcemia especially ionised calcium in asphyxiated infants and high frequency of functional derangement associated with this hypocalcemioa, serial monitoring of serum isonised calcium levels is necessary.     

Dissociation as a mediator between child abuse history and adult abuse potential

OBJECTIVE: It has commonly been found that abused children are at risk for later becoming abusive parents (Kaufman & Zigler. 1987; Oliver, 1993) and observational learning has been discussed as a mechanism that perpetuates this intergenerational cycle of abuse. However, two thirds of abused children do not become abusive (Kaufman & Zigler, 1987). Thus, the goal of the current study was to examine whether dissociation functions as an additional mechanism mediating the relation between a history of child abuse and abusiveness as an adult. METHOD: A cross-sectional design was used to examine physical abuse history, dissociation, and physical abuse potential in a sample of 190 college students. Questionnaires were utilized to assess the three constructs. RESULTS: Findings indicated that the three constructs were intercorrelated. In particular, a newly established association was found between dissociation and physical abuse potential (r = .54, p < .0001). The primary finding was that the relation between physical abuse history and physical abuse potential was significantly mediated (z = 2.19, p < .05) by level of dissociation, with dissociation accounting for approximately half of the observed relation between history of abuse and abuse potential. CONCLUSION: Results suggested that dissociation may be one mechanism that helps to perpetuate the intergenerational cycle of abuse. Although dissociation promotes psychological survival during children's abuse experiences, it may result in the development of abusive tendencies in later life. Reducing parental dissociation may assist clinicians in preventing or terminating physical child abuse.