Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

College Student Feedback About Librarian-Created Instructional Videos: Why Aren't They Using Them?

Library instruction videos can enhance student instruction. At a community college, librarian-created videos provided students with academic support. However, the videos were rarely used. In this article, students were sent online surveys to rate videos discussing the American Psychological Association (APA) format. Students were also asked why they did not use the videos. Participants rated several video features, including length, technical aspects, narration, and style. While they overwhelmingly responded that the videos were useful, the students did not know where they were located. The major complaint was related to the videos' technical aspects. Students also reported that they were more inclined to ask for help after watching the video.     

Industrial Experience Projects: A Balance of Process and Product

Students in the final year of the Bachelor of Computing degree at Monash University must do a full year group project as a capstone subject. Each group of five or six builds a real system which is normally for a client outside the university. The project usually necessitates learning some skills that have not been taught in the course. Each group works on a different project. The emphasis is on management issues and the software development process as much as on the product, with a set of deliverables and deadlines throughout the year, and an assessment strategy reflecting this.     

The International Network for Older Adults

Conclusion Although funds were not available to bring members of the Older Adults Network to the World Assembly of Adult Education in January 1990, there was, among the delegates, considerable interest in the subject and several meetings were arranged. The thing that struck me most was not how different were our situations, coming as we did from every corner of the earth, but how many problems and concerns we had in common.With the second Network Newsletter, sent out in the spring of 1991, questionnaires asked for brief details of schemes which involved older people in projects that were, in some way, conservational. They could be involved in conserving language, mythology or history. They might be working to improve and save their environment. The aim is to establish a small but useful register of such projects in sufficient detail to encourage contact and replication by others. For this purpose, small grants are being made available from the money given by CIDA. Slowly but surely, the Older Adults Network is gathering information about positive actions being taken to ensure that older people, in all countries, have the skills and opportunities they need to continue as fully participating citizens. With the rapidly increasing number of older people in all our countries, this small beginning will, hopefully, be a foundation on which much important work will be done in the years to come.     

Trade books and the social studies curriculum

Historical fiction, fiction about contemporary people of other cultures, and nonfiction trade books can increase children's interest in social studies and enhance their knowledge. Dianne Monson and Kathleen Howe discuss the ways that these books appeal to young readers and lead them to seek further understanding of historical events, foreign cultures, and Americans from different cultural backgrounds. They offer numerous examples of books that serve this purpose for several social studies topics. Kathleen Howe completed her doctorate at the University of Minnesota. Her dissertation dealt with the use of children's literature trade books in the elementary social studies curriculum. Dianne Monson is a past president of the U.S. Board on Books for Young People and has written and lectured on children's literature throughout the curriculum.     

Physical activities and sedentary pursuits in African American and Caucasian girls

The purposes of this study were to describe and compare the specific physical activity choices and sedentary pursuits of African American and Caucasian American girls. Participants were 1,124 African American and 1,068 Caucasian American eighth-grade students from 31 middle schools. The 3-Day Physical Activity Recall (3DPAR) was used to measure participation in physical activities and sedentary pursuits. The most frequently reported physical activities were walking, basketball, jogging or running, bicycling, and social dancing. Differences between groups were found in 11 physical activities and 3 sedentary pursuits. Participation rates were higher in African American girls (p < or = .001) for social dancing, basketball, watching television, and church attendance but lower in calisthenics, ballet and other dance, jogging or running, rollerblading, soccer, softball or baseball, using an exercise machine, swimming, and homework. Cultural differences of groups should be considered when planning interventions to promote physical activity.