Toxic effects of Litsea elliptica Blume essential oil on red blood cells of Sprague-Dawley rats

Litsea elliptica Blume leaves have been traditionally used as medicinal herbs because of its antimutagenicity, che-mopreventative and insecticidal properties. In this study, the toxic effects of L. elliptica essential oil against Sprague-Dawley rat's red blood cells (RBCs) were evaluated. L. elliptica essential oil was given by oral gavage 5 times per week for 3 treated groups in the doses of 125, 250, and 500 mg/(kg body weight), respectively, and the control group received distilled water. Full blood count, RBC osmotic fragility, RBC morphological changes, and RBC membrane lipid were analyzed 28 d after the treatment. Although L. elliptica essential oil administration had significantly different effects on hemoglobin (Hb), mean cell hemoglobin concentration (MCHC), mean cell volume (MCV), and mean cell hemoglobin (MCH) in the experimental groups as compared to the control group (P<0.05), the values were still within the normal range. L. elliptica induced morphological changes of RBC into the form of echinoeyte. The percentage of echinocyte increased significantly among the treated groups in a dose-response manner (P<0.001). The concentrations of RBC membrane phospholipids and cholesterol of all treated groups were significantly lower than those of control group (P<0.001). However, the RBC membrane osmotic fragility and total proteins of RBC membrane findings did not differ significantly between control and treated groups (P>0.05). It is concluded that structural changes in the RBC membrane due to L. elliptica essential oil administration did not cause severe membrane damage.     

Epigallocatechin-3-gallate affects the growth of LNCaP cells via membrane fluidity and distribution of cellular zinc

Objective: To evaluate effects of epigallocatechin-3-gallate (EGCG) on the viability, membrane properties, and zinc distribution, with and without the presence of Zn2+, in human prostate carcinoma LNCaP cells. Methods: We examined changes in cellular morphology and membrane fluidity of LNCaP cells, distribution of cellular zinc, and the incorporated portion of EGCG after treatments with EGCG, Zn2+, and EGCG+Zn2+. Results: We observed an alteration in cellular morphology and a decrease in membrane fluidity of LNCaP cells after treatment with EGCG or Zn2+. The proportion of EGCG incorporated into liposomes treated with the mixture of EGCG and Zn2+ at the ratio of 1:l was 90.57%, which was significantly higher than that treated with EGCG alone (30.33%). Electron spin resonance (ESR) studies and determination of fatty acids showed that the effects of EGCG on the membrane fluidity of LNCaP were decreased by Zn2+. EGCG accelerated the accumulation of zinc in the mitochondria and cytosol as observed by atomic absorption spectrometer. Conclusion: These results show that EGCG interacted with cell membrane,decreased the membrane fluidity of LNCaP cells, and accelerated zinc accumulation in the mitochondria and cytosol, which could be the mechanism by which EGCG inhibits proliferation of LNCaP cells. In addition, high concentrations of Zn2+ could attenuate the actions elicited by EGCG.     

Perspective: Flicking with flow: Can microfluidics revolutionize the cancer research?

According to the World Health Organization, cancer is one of the leading causes of death worldwide. Cancer research, in its all facets, is truly interdisciplinary in nature, cutting across the fields of fundamental and applied sciences, as well as biomedical engineering. In recent years, microfluidics has been applied successfully in cancer research. There remain, however, many elusive features of this disease, where microfluidic systems could throw new lights. In addition, some inherent features of microfluidic systems remain unexploited in cancer research. In this article, we first briefly review the advancement of microfluidics in cancer biology. We then describe the biophysical aspects of cancer and outline how microfluidic system could be useful in developing a deeper understanding on the underlying mechanisms. We next illustrate the effects of the confined environment of microchannel on cellular dynamics and argue that the tissue microconfinement could be a crucial facet in tumor development. Lastly, we attempt to highlight some of the most important problems in cancer biology, to inspire next level of microfluidic applications in cancer research.     

The counseling needs of foreign students

This paper reports the findings of three studies of the counseling needs of international students at a medium sized University in the Midwestern region of USA. The studies were conducted over a period of five years (1977–83), and provide an historical perspective on the changes in student needs as well as in the programs designed to respond to these needs.     

The earth’s changing climate

Various human activities have contributed to an increase in the levels of greenhouse gases. The contribution that these make to global warming requires further investigation, because there are several negative feedback mechanisms which inhibit the warming process.     

Policy versus ground reality: secondary English language assessment system in Bangladesh

Any policy reform in education is highly effective when it is planned and implemented ‘holistically’ and yet, it is the most challenging way forward. Many countries in Asia have reformed their English language policies and syllabi in the last two decades due to the increasing value of the language worldwide. Motivated by a ‘communicative approach’ to English language teaching, Bangladesh was one of the countries that launched such a reform in the 1990s. However, this reform has been criticised for imposing the changes on teachers without preparing them sufficiently. More importantly, there is limited evidence as to how much the secondary English language assessment system is aligned with the changes introduced in the curriculum. In order to explore this gap, a medium-scale study was conducted in 38 secondary schools in Bangladesh, following a mixed methods approach. The findings highlighted a ‘mismatch’ between the intended English language learning outcomes and current assessment practices, including the resulting challenges faced by the teachers. This paper argues that language education reform is likely to fail if the assessment system is not aligned with the curriculum.     

Projectile motion with quadratic damping in a constant gravitational field

The motion of a projectile in a constant gravitational field is an age-old problem. If there is no resistive force, the problem is simple. However, with the intoduction of resistive forces, the complexity of the problem increases. Recently, there has been a growing interest in the problem of projectile motion under a resistive force proportional to the square of velocity. The problem for resistive force proportional to vv cannot be solved in closed analytical form. A series solution of the equation leads to divergent series for x (t) and y (t) for reasonable values of the initial speed and angle of projection. However, we demonstrate that by inverting the series for x (t), i.e., expressing t in terms of x, and substituting this in y (t) one can express y in terms of x which is a highly convergent series and one can obtain the path of projectile with a few terms of the series. We further demonstrate that one can obtain the path even with a few terms of the divergent series for x (t) and y (t) by using extrapolation methods.     

Meaning at the Interface: New Genres,New Modes of Interpretative Engagement?

As audience reception scholars commute from audiences to literacies, both of which share the concept of interpretation, this article sets out to draw together themes around the changing nature of interpretive work, from 6 texts that have reflected recently, in different ways, on interpretation in new media environments. In reviewing a multithreaded conversation across 4 books from literacy studies, media and communications, and user studies, I select 2 essays, written at a crucial moment for reception studies, to provide her a narrative. Speaking from diverse fields, these works suggest a widespread agreement that there is a need to rethink terminologies and concepts from mass-mediated communication in the age of interactive media, not because they no longer prove useful, but because they provide constructive intellectual challenges in thinking through the changing natures of texts and readers, genres and interpretation, literacies, and legibilities, as we await new modes every day, for all of these, but also perhaps, new modes of interpretative engagement.