The impact of microfluidics in high-throughput drug-screening applications

Drug discovery is an expensive and lengthy process. Among the different phases, drug discovery and preclinical trials play an important role as only 5–10 of all drugs that begin preclinical tests proceed to clinical trials. Indeed, current high-throughput screening technologies are very expensive, as they are unable to dispense small liquid volumes in an accurate and quick way. Moreover, despite being simple and fast, drug screening assays are usually performed under static conditions, thus failing to recapitulate tissue-specific architecture and biomechanical cues present in vivo even in the case of 3D models. On the contrary, microfluidics might offer a more rapid and cost-effective alternative. Although considered incompatible with high-throughput systems for years, technological advancements have demonstrated how this gap is rapidly reducing. In this Review, we want to further outline the role of microfluidics in high-throughput drug screening applications by looking at the multiple strategies for cell seeding, compartmentalization, continuous flow, stimuli administration (e.g., drug gradients or shear stresses), and single-cell analyses.     

Effects of interval aerobic training combined with strength exercise on body composition,glycaemic and lipid profile and aerobic capacity of obese rats

The purpose of this study was to investigate the effects of interval aerobic training combined with strength exercise in the same training session on body composition, and glycaemic and lipid profile in obese rats. Sixteen lean Zucker rats and sixteen obese Zucker rats were randomly divided into exercise and sedentary subgroups (4 groups, n = 8). Exercise consisted of interval aerobic training combined with strength exercise in the same training session. The animals trained 60 min/day, 5 days/week for 8 weeks. Body composition, lipid and glycaemic profiles and inflammatory markers were assessed.

Results showed that fat mass was reduced in both lean and obese rats following the exercise training (effect size (95% confidence interval (CI)) = 1.8 (0.5–3.0)). Plasma low-density lipoprotein–cholesterol and fasting glucose were lower in the exercise compared to the sedentary groups (d = 2.0 (0.7–3.2) and 1.8 (0.5–3.0), respectively). Plasma insulin was reduced in exercise compared to sedentary groups (d = 2.1 (0.8–3.4)). Some exercise × phenotype interactions showed that the highest decreases in insulin, homeostatic model assessment-insulin resistance, fasting and postprandial glucose were observed in the obese + exercise group (all, P < 0.01). The findings of this study suggest that interval aerobic training combined with strength exercise would improve body composition, and lipid and glycaemic profiles, especially in obese rats.     

Many choices,one destination: multimodal university brand construction in an urban public transportation system

Amidst global competition in higher education, colleges and universities adopt strategies that mimic and adapt business practices. Branding is now a widespread practice in higher education; multimodal advertisement is a manifestation of emerging branding strategies for universities. While the visibility of brands in higher education has grown substantially in recent years, its empirical study has lagged. This article reports on the findings from a study that employed social semiotic and multimodal analysis to explore text and visual rhetoric as brand construction strategies in publicly displayed university advertisements. After analyzing photographs representing advertising campaigns from 16 different universities displayed in an urban public transportation system, I suggest that universities construct their brand identity through messages that emphasize multiple choices and convenience, but construct success primarily according to corporate standards and values.     

Prostate specific antigen in patients of benign prostate hypertrophy and carcinoma prostate

Prostate Specific Antigen (PSA) has emerged as the most applicable and important tumor marker for carcinoma prostate. In the present study PSA was determined in serum of healthy subjects, patients of benign prostate hypertrophy (BPH) and Carcinoma Prostate (Ca−P) to evaluate its diagnostic efficiency in day to day management of prostate cancer patients and in differentiating patients of early prostate cancer from those with BPH. Receiver operating characteristic curve (ROC) revealed 2 ng/ml and 10 ng/ml cut off serum PSA level for BPH and untreated carcinoma prostate patients (Ca−P). An extremely significant increase (P<0.0001) was observed in mean PSA concentration in BPH patients and adenocarcinoma prostate patients when compared to healthy males. Clinical relevance of PSA was highlighted by a case study of cancer patient prior to any therapy till death.     

Cardiorespiratory and perceptual responses of two interval training and a continuous training protocol in healthy young men

High Intensity Interval Training (HIIT) can be performed with different effort to rest time-configurations, and this can largely influence training responses. The purpose of the study was to compare the acute physiological responses of two HIIT and one moderate intensity continuous training (MICT) protocol in young men. A randomised cross-over study with 10 men [age, 28.3?±?5.5years; weight, 77.3?±?9.3?kg; height, 1.8?±?0.1?m; peak oxygen consumption (VO₂peak), 44?±?11?mL.kg^?1.min^?1]. Participants performed a cardiorespiratory test on a treadmill to assess VO₂peak, velocity associated with VO₂peak (vVO₂peak), peak heart rate (HRpeak) and perceived exertion (RPE). Then participants performed three protocols equated by distance: Short HIIT (29 bouts of 30s at vVO₂peak, interspersed by 30s of passive recovery, 29?min in total), Long HIIT (3 bouts of 4?min at 90% of vVO₂peak, interspersed by 3?min of recovery at 60% of vVO₂peak, 21?min in total) and MICT (21?min at 70% of vVO₂peak). The protocols were performed in a randomised order with ≥48 h between them. VO₂, HRpeak and RPE were compared. VO₂peak in Long HIIT was significantly higher than Short HIIT and MICT (43?±?11 vs 32?±?8 and 37?±?8?mL.kg^?1.min^?1, respectively, P?P?P?2, HR and RPE than Short HIIT and MICT, suggesting a higher demand on the cardiorespiratory system. Short HIIT and MICT presented similar physiologic and perceptual responses, despite Short HIIT being performed at higher velocities.     

Experiences of Danish Business Expatriates in Russia: Power Distance in Organizational Communication

Rapid globalization of business processes stimulates many companies to dispatch their employees – business expatriates – on overseas assignments. Russia differs markedly from many other cultures in terms of specific communication norms and rules. Understanding such differences in organizational life is crucial for the success of business expatriates working there. This study employs in-depth interviews with eight Danish business expatriates who worked in Russia and examines the differences between the Danes and the Russians in terms of communication characteristics related to Hofstede’s cultural dimension power distance. This work enhances scholars’ empirical knowledge of power distance, which has rarely been investigated qualitatively.     

Electrical and mechanical response of skeletal muscle to electrical stimulation after acute passive stretching in humans: a combined electromyographic and mechanomyographic approach

Two mechanisms have been suggested to explain stretching-induced maximum force depression: a mechanical alteration in the stretched muscle and an impairment of neural activation. Electrical stimulation allows standardization of the level of muscle activation without being limited by neural control. The aim of this study was to evaluate the stretching-induced changes in the electrical and mechanical properties of muscle during electrically elicited contractions. Twelve participants (age 22 +/- 1 years; body mass 75 +/- 2 kg; stature 1.79 +/- 0.02 m; mean +/- standard error) underwent six electrical stimulations of the medial gastrocnemius muscle before and after stretching. During the contractions, surface electromyogram (EMG) and mechanomyogram (MMG) were recorded simultaneously together with force. After stretching we found: (i) no differences in EMG parameters; (ii) MMG amplitude decreased by 4 +/- 1% (P < 0.05); and (iii) the peak force, the peak rate of force development, and the acceleration peak of force development decreased by 12 +/- 3%, 14 +/- 1%, and 24 +/- 5%, respectively (P < 0.05). In conclusion, acute passive stretching did not change EMG properties but altered the mechanical characteristics of the contracting muscle. Indeed, muscle force-generating capacity and stiffness of the muscle-tendon unit were significantly impaired.     

Specific tackling situations affect the biomechanical demands experienced by rugby union players

Tackling in Rugby Union is an open skill which can involve high-speed collisions and is the match event associated with the greatest proportion of injuries. This study aimed to analyse the biomechanics of rugby tackling under three conditions: from a stationary position, with dominant and non-dominant shoulder, and moving forward, with dominant shoulder. A specially devised contact simulator, a 50-kg punch bag instrumented with pressure sensors, was translated towards the tackler (n = 15) to evaluate the effect of laterality and tackling approach on the external loads absorbed by the tackler, on head and trunk motion, and on trunk muscle activities. Peak impact force was substantially higher in the stationary dominant (2.84 ± 0.74 kN) than in the stationary non-dominant condition (2.44 ± 0.64 kN), but lower than in the moving condition (3.40 ± 0.86 kN). Muscle activation started on average 300 ms before impact, with higher activation for impact-side trapezius and non-impact-side erector spinae and gluteus maximus muscles. Players’ technique for non-dominant-side tackles was less compliant with current coaching recommendations in terms of cervical motion (more neck flexion and lateral bending in the stationary non-dominant condition) and players could benefit from specific coaching focus on non-dominant-side tackles.     

Natural lighting in the Hall of Two Hundred. A proposal for exhibition of its ancient tapestries

The present paper provides a qualitative and quantitative approach to natural lighting design for the Hall of Two Hundred in Palazzo Vecchio (the present town hall) in Florence. Starting from transient simulation of natural lighting inside the Hall in its present state, using the commercial software Radiance, a design proposal was suggested, detecting the optimal conditions for tapestry conservation and maintenance and taking into account their periodical exhibition, designing a proper wall show-case solution. The suggested design does not modify the architecture and structure of the building. The distribution of natural lighting and consequently of the illuminance and luminance inside the ambient, suggested a solution for exhibition of the historical tapestries, which can guarantee not only tapestry conservation but also the Hall structure and its present uses. The design proposal provides a comfortable lighting environment, rehabilitation and improvement of the historical perception and sight of these works of art, under variable sun and sky conditions throughout the year.