Paper-based colorimetric enzyme linked immunosorbent assay fabricated by laser induced forward transfer

We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care diagnostic sensors. To minimise the loss of functionality of the active biomolecules during transfer, a dynamic release layer was employed to shield the biomaterial from direct exposure to the pulsed laser source. Cellulose paper was chosen as the ideal receiver because of its inherent bio-compatibility, liquid transport properties, wide availability and low cost, all of which make it an efficient and suitable platform for point-of-care diagnostic sensors. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed and their functionality was confirmed via a colorimetric enzyme-linked immunosorbent assay. Localisation of the printed antibodies was exhibited, which can allow the creation of complex 2-d patterns such as QR codes or letters for use in a final working device. Finally, a calibration curve was determined that related the intensity of the colour obtained to the concentration of active antibodies to enable quantitative assessment of the device performance. The motivation for this work was to implement a laser-based procedure for manufacturing low-cost, point-of-care diagnostic devices on paper.     

The value of instrumental and affective communication skills in different relational stages

The focus of this study was to assess the value that college students place on instrumental and affective communication skills across progressive relational stages in same‐sex relationships. Existing measures were utilized for affective and instrumental skills, however a newly developed communication skill (companionship) was also included. Relational stages included non‐unit (acquaintance), pre‐unit (testing friendship potential), unit (friend), and super‐unit (special friend). Results indicated that participants in non‐unit relationships valued some affective communication skills less tlian did those in pre‐unit, unit, and super‐unit relationships. All relational stages valued instrumental skills equally. Some gender differences were noted.     

Residing in silence and wonder: career counselling from the perspective of ‘being’

We are approaching career counselling from the perspective of “Being”, which we recognize to be a daunting task. Nevertheless we find it extremely important that the counsellor become aware of and try to think, talk and act from his or her embeddedness in being, and by that help the client to do the same. This process involves an ontological and spiritual approach, and builds upon the Socratic ability to wonder, the meditative way of “undoing” and the artful act of creation.     

Placement schools as professional learning communities in teacher education

This article investigates how schoolteachers’, school leaders’ and college teachers’ involvement affects placement schools as professional learning communities. Norwegian teacher education is used as a case. The first part builds on a survey among schoolteachers and mentors at 111 placement schools in Norway. It documents great variety in the level of engagement. Interviews with mentors, school leaders and college teachers reveal how cooperation between colleges and placement schools, as well as the school leaders’ commitment, influences the quality of placement. The school leaders’ role proves to be important in developing the schools as professional learning communities, and they seem to have a significant impact on the work of the mentors. The data also show that there is a need for a more substantial cooperation between college teachers and mentors about the student teachers’ professional development as well as a need for a more systematic integration of learning in the two learning contexts.     

Development of motor-life-skills: variations in children at risk for motor difficulties from the toddler age to preschool age

This article explores variations in development of everyday motor-life-skills in 661 children (329 girls and 332 boys) in Norwegian kindergartens of ages 2:9 (T1) and 4:9 (T2) years:months. The particular focus is on children at risk for problems in motor development (the 10% weakest children in the sample). The methodological approach chosen is authentic assessment, applying the Early Years Movement Skills Checklist (EYMSC). All correlations between motor-life-skills at ages 2:9 and 4:9 are statistically significant (p < 0.01), varying between r = 0.26 to 0.38 for the four section scores of EYMSC (Self-help skills, Desk skills, General classroom skills and Recreational and playground skills) and r = 0.39 for the EYMSC total score. The group composition of children assumed to be at risk for motor difficulties changes considerably between ages 2:9 and 4:9. Approximately, two-thirds of the 10% weakest at T1 do not belong to the 10% weakest at T2. Logistic regression failed to identify children at risk at T1 being among the 10% weakest at T2. However, for two sections of EYMSC (Self-help skills; Recreational and Playground skills), it was possible to distinguish between stable and flux groups.     

Why nurturing curiosity is an ethical endeavour: exploring practitioners’ reflections on the importance of curiosity

Several authors have argued that early childhood education and care (ECEC) is essentially an ethical practice based on certain values and conceptions about the purpose of education. Is this also the case for educational practices surrounding the highly valued notion of curiosity? While psychological approaches portray it as neutral, this article aims to explore curiosity's normative connotations. To do so, an ethnographically informed approach and video-stimulated recall focus-group interviews have been used to gather Norwegian ECEC practitioners' reflections on the importance of curiosity. The reflections have been analysed using the concept of ethical rationalities and can be grouped into four approaches to valuing curiosity: the relational approach, the virtue approach, the consequentialist approach and the essentialist approach. The analysis suggests that different practices for nurturing curiosity are constrained and others are enabled, depending on the preferred ethical rationality. To prevent the nurturing of curiosity from being based on an assumed and restricted range of theoretical and ethical perspectives, it is necessary to invite practitioners’ reflections on values associated with curiosity.

SHORT ABSTRACT

While psychological approaches portray it as neutral, this article aims to explore curiosity's normative connotations in the daily practices of ECEC. To do so, an ethnographically informed approach and video-stimulated recall focus-group interviews have been used to gather practitioners' reflections on the importance of nurturing curiosity. These have been analysed using the concept of ethical rationalities and can be grouped into four approaches to valuing curiosity: the relational approach, the virtue approach, the consequentialist approach and the essentialist approach. To prevent the nurturing of curiosity from being based on a restricted range of theoretical and ethical perspectives, it is necessary to invite reflection on its value.     

The effect of plot explicit,educational explicit,and implicit inference information and coviewing on children's internal and external cognitive processing

Parental coviewing – the act of being present when a child is watching television – can influence the child’s cognitive processing and emotional reactions. This study investigated the role coviewing has on the child’s cognitive processing – which is evidenced by the phasic psychophysiological orienting response to three types of information: plot explicit, educational explicit, and implicit inference. An experiment was conducted that measured the heart rate of children (N?=?88; mean age?=?9.12 years) while watching messages either with or without a parent present in the room. It was predicted, and found, that coviewing leads to greater resource allocation to encoding the message – as indicated by phasic cardiac deceleration, and that information that required internal processing, such as plot explicit or implicit inferential content, leads to greater resources allocated to internal processing – as indicated by phasic cardiac acceleration. Implications for parental mediation strategies and educational television programming are given.     

Relational transitions,emotional decisions: new directions for theorising graduate employment

University-to-work transitions tend to be discussed in terms of skills, outcomes and the readiness of graduates for an increasingly insecure and flexible labour market. Such a focus on individual attributes and orientations depicts graduates as lonely and ostensibly rational figures; disembedded from their intimate networks and devoid of emotional context as they navigate their post-university pathways. This article aims to steer the debate in a new, fundamentally relational direction by exploring the role and significance of intimate kin and non-kin relationships for the ways graduates experience and make choices about employment and careers. Drawing on qualitative longitudinal research with women who graduated from universities in the UK between 2009 and 2011, the discussion highlights the value of an explicitly relational perspective for revealing the personal and emotional dimensions of the transition out of higher education. The article concludes that the process of securing work and committing to a career is embedded within the broader experiences of personal life, emotion and (im)mobility and, thus, raises important questions about the role and responsibility of universities at a time when employability metrics are read as a marker of teaching quality.     

The impact of color and role on retention of knowledge: a body-painting study within undergraduate medicine

Body painting as a tool for teaching anatomy is becoming increasingly popular as it is fun and diffuses the formal academic context. Students claim bright color plays a significant role in retention of knowledge from painting sessions. Medical students (n = 117) were divided into two conditions: block color (CLR) and black outlines (BLK). Students completed a pre-test before a body painting teaching session on abdominal referred pain. CLR students used bright block colors, and BLK students mapped outlines in black. Immediately afterward, students sat a mid-test to compare the conditions. Students completed a post-test to compare long-term retention of knowledge four weeks later. There was no difference in knowledge between the two conditions immediately after the teaching (P = 0.653). There was no significant difference in long-term retention of knowledge between those using black outlines and those using color (P = 0.278). The use of color had no impact on short-term or long-term retention of knowledge, despite previously collected qualitative data that color helped memorization. Despite there being no immediate difference in the amount of information retained, the students' enthusiasm for body painting and the use of bold colors warrant body painting's inclusion within the anatomy curriculum. Acceptability plays a significant role in the success of any teaching modality. Additionally, students who undertook the roles of painter or canvas retained similar amounts of information after six weeks (P = 0.505). Students' classification as verbalizer or visualizer did not impact on their test performance.