全文获取类型
收费全文 | 4374篇 |
免费 | 81篇 |
国内免费 | 120篇 |
专业分类
教育 | 3016篇 |
科学研究 | 520篇 |
各国文化 | 6篇 |
体育 | 450篇 |
综合类 | 371篇 |
文化理论 | 9篇 |
信息传播 | 203篇 |
出版年
2024年 | 6篇 |
2023年 | 16篇 |
2022年 | 51篇 |
2021年 | 112篇 |
2020年 | 83篇 |
2019年 | 47篇 |
2018年 | 29篇 |
2017年 | 50篇 |
2016年 | 34篇 |
2015年 | 113篇 |
2014年 | 212篇 |
2013年 | 238篇 |
2012年 | 223篇 |
2011年 | 299篇 |
2010年 | 282篇 |
2009年 | 287篇 |
2008年 | 269篇 |
2007年 | 305篇 |
2006年 | 285篇 |
2005年 | 255篇 |
2004年 | 250篇 |
2003年 | 260篇 |
2002年 | 333篇 |
2001年 | 272篇 |
2000年 | 148篇 |
1999年 | 42篇 |
1998年 | 28篇 |
1997年 | 7篇 |
1996年 | 12篇 |
1995年 | 6篇 |
1994年 | 5篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1989年 | 3篇 |
1985年 | 2篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
排序方式: 共有4575条查询结果,搜索用时 15 毫秒
41.
Objective: To determine the effective nucleotide sites of ribozymes against HCV, and obtain a highly effective, nontoxic and
inexpensive antisense ribozyme specific for HCV. Methods: Two effective ribozymes, targeted to HCV 5’NC region and C region,
were designed and synthesized. Eukaryotic expression vectors, pSV2-gpt. CD-SR (, containing either HCRZNC or HCRZC were constructued
and transfected into MT-2 cells, which had been infected by HCV. Quantitative PCR and hydridization were used to determine
the effect of inhibition of HCV by ribozymes. Results: HCRZNC and HCRZC suppressed the replication of HCV by 54.7% and 62.1%,
respectively. Furthermore, when both ribozymes were cotransfected into cells, they suppressed replication by 78.8%. Conclusion:
Two specific antisense ribozymes have strong inhibitory effects on the replication of HCV in cultured cells, and have better
effect when used together. 相似文献
42.
采用问卷调查法分析教师职业价值观与教师职业倦怠的关系.结果显示,不同职业价值观的教师其职业倦怠程度具有显著的差异性,教师的职业价值观与其职业倦怠的程度呈显著的正相关,教师的职业价值观对其职业倦怠程度具有一定的影响和预测作用. 相似文献
43.
Roland Barthes,the outstanding thinker as well as the semiologist of France,has left great contribution to us human beings.His The Death of the Author made a strong,polemical argument against the centrality of the figure of the author in literary study.It states that the intentions of the author are meaningless to the interpretation of a text.Michel Foucault,the famous French philosopher and the historian in the ideology field,in his What is An Author,shows the idea of the author,as a timeless,irreducible category,is,rather,a "function" of discourse which has changed in the course of history.The author means quite different in their ideas.But what the author really is? 相似文献
44.
Mobile devices are increasingly powerful in multimedia transmitting and browsing. However, the small screen and different
aspect ratios of mobile devices lower visual quality while watching images or videos. Video retargeting is aim at fitting
an existing video into arbitrary size and aspect ratio. Previous content-aware retargeting methods are mostly high computational
cost, which limits their applications on the portable devices with low computation ability. In this paper, a new crop-and-scale
approach is presented to adapt video to better suit the target display. We automatically find the optimal parameters of cropping
window using the dynamic programming method, and then scale it to fit the target display. The cropping window can smoothly
shift during a shot to follow the movement of important objects. The retargeting results using our approach introduce no deformation
and jitter effects over the whole video. Experimental results show the success of our approach on adapting a variety of source
videos to small display sizes. 相似文献
45.
对全国14所重点高校2800位学术型研究生进行培养模式运行状况的专题调研,调研内容包括学生基本信息、招生环节、培养过程、考核评价、生活情况、自我评价、对策建议七个方面。调研发现,有利于培养创新能力的课程比例不高,学生首次参加课题研究的时间过迟,毕业论文选题准备不足,独立完成能力不强,高水平科研成果偏少。以培养拔尖创新能力为目标完善研究生培养模式仍是高校重要任务,要建立学术本科-学术硕士-学术博士一条龙研究性培养对接体系,以"三题"(课题、专题、问题)研究接力提升学生创新能力;建立责权利相统一的考评体系,使导师的主要时间精力放在人才培养上;开设研究性、前沿性、创新性课程,着力实施探究式教学模式;大幅增加研究生教育投入,培育创新性研究环境和氛围,使导师和学生成为令人羡慕的知识创新共同体。 相似文献
46.
47.
朱智 《黄冈职业技术学院学报》2020,(1):62-65
非物质文化遗产需要传承和弘扬,地方旅游业发展需要文化支撑,如何将二者融合发展,值得研究。本文收集、整理湖州非物质文化遗产资料,对其数量、类型、分布等进行分析,并实地调查了湖州旅游发展过程中对非物质文化遗产的利用现状,提出湖州乡村旅游应充分利用非物质文化遗产,可从打造舞台盛宴、创新导游解说、打造非遗属性旅游纪念品三个方面提升乡村旅游的文化内涵。这样,既可以更好地保护地方非物质文化遗产,又可以推动地方旅游文化事业,二者的结合是双赢之举。 相似文献
48.
Objective: To investigate the effect of interleukin-18 (IL-18) on immune response induced by plasmid encoding hepatitis B virus middle protein antigen and to explore new strategies for prophylactic and therapeutic HBV DNA vaccines. Methods: BALB/c mice were immunized with pCMV-M alone or co-immunized with pcDNA3-18 and pCMV-M and then their sera were collected for analysing anti-HBsAg antibody by ELISA; splenocytes were isolated for detecting specific CTL response and cytokine assay in vitro. Results: The anti-HBs antibody level of mice co-immunized with pcDNA3-18 and pCMV-M was slightly higher than that of mice immunized with pCMV-M alone, but there was not significantly different (P>0.05). Compared with mice injected with pCMV-M, the specific CTL cytotoxity activity of mice immunized with pcDNA3-18 and pCMV-M was significantly enhanced (P<0.05) and the level of IFN-γ in supernatant of splenocytes cultured with HBsAg in vitro was significantly elevated (P<0.05) while the level of IL-4 had no significant difference (P>0.05). Conclusion: The plasmid encoding IL-18 together with HBV M gene DNA vaccines may enhance specific TH1 cells and CTL cellular immune response induced in mice, so that IL-18 is a promising immune adjuvant. 相似文献
49.
耿曼子 《伊犁教育学院学报》2006,19(4):175-177
类比法是一种重要的思维方法和推理方法,它在数学领域中发挥的作用无处不在.通过几个例子说明它在立体几何中的运用,在数学的学习与研究中,应该重视类比法的运用. 相似文献
50.
2018年《关于全面深化新时代教师队伍建设改革的意见》对教师继续教育提出了新要求,也为思政课教师的继续教育指明新方向和创新的新源泉.因此,文章在基于高校思政课教师继续教育现状分析的基础上,依据文件精神,紧紧把握政治方向,将文件精神融入继续教育全过程中,提出立德树人,强化继续教育德育功能;融合情景教育教学,构建"逆序创新"教育模式等对策,以解决新时代高校思政课教师继续教育所存在的问题. 相似文献