Pheromone synthesis in a biomicroreactor coated with anti-adsorption polyelectrolyte multilayer

To prepare a biosynthetic module in an infochemical communication project, we designed a silicon/glass microreactor with anti-adsorption polyelectrolyte multilayer coating and immobilized alcohol acetyl transferase (atf), one of the key biosynthetic enzymes of the pheromone of Spodoptera littoralis, on agarose beads inside. The system reproduces the last step of the biosynthesis in which the precursor diene alcohol (Z,E)-9,11-tetradecadienol is transformed into the major component (Z,E)-9,11-tetradecadienyl acetate. The scope of this study was to analyze and implement a multilayer, anti-adsorption coating based on layer-by-layer deposition of polyethylenimine/dextransulfate sodium salt (PEI/DSS). The multilayers were composed of two PEI with molecular weights 750 and 1.2 kDa at pH 9.2 or 6.0. Growth, morphology, and stability of the layers were analyzed by ellipsometry and atomic force microscopy (AFM). The anti-adsorption functionality of the multilayer inside the microreactor was validated. The activity of His(6)-(atf) was measured by gas chromatography coupled to mass spectrometer (GC-MS).     

Disposable parallel poly(dimethylsiloxane) microbioreactor with integrated readout grid for germination screening of Aspergillus ochraceus

In this work a disposable, parallel microbioreactor (MBR) suitable for screening in batch or continuous mode is presented. The reactor consists of five parallel microchambers made of poly(dimethylsiloxane) bonded to a glass substrate. A grid structure is engraved on each chamber, allowing subsequent morphology imaging. Measurements are recorded over the entire cultivation period with constant parameters, namely, position and focus in the z-axis. The microdevice may be used for either parallel, uni- or multiparametric screening, and overcomes the drawback of gridless microwell plates which require expensive equipment such as an inverted microscope with an automatic stage. To validate the scalability from laboratory scale to microscale, and thus the cultivation protocol in the MBR, the germination of fungal spores (A. ochraceus) is evaluated for two different key magnitudes (pH and temperature) and compared to the results obtained from conventional laboratory scale systems (flasks and agar plates). Information on germination capacity with regard to interspecies' variability allows for optimization of industrial processes as optimal pH and temperature matched to the mesoscopic cultivation systems. The germination conditions therefore remain unaffected inside the MBR, while providing the following advantages: (i) dramatic reduction of medium consumption, (ii) submerged cultivation with constant oxygen supply, (iii) assured low cost and disposability, and (iv) possibility of a continuous cultivation mode.     

A comparison of anthropometric and training characteristics of Ironman triathletes and Triple Iron ultra-triathletes

We examined differences in anthropometry and training between 64 Triple Iron ultra-triathletes competing over 11.4 km swimming, 540 km cycling, and 126.6 km running, and 71 Ironman triathletes competing over 3.8 km swimming, 180 km cycling, and 42.2 km running. The association of anthropometry and training with race time was investigated using multiple linear regression analysis. The Triple Iron ultra-triathletes were smaller (P < 0.05), had shorter limbs (P < 0.05), a higher body mass index (P < 0.05), and larger limb circumferences (P < 0.01) than the Ironman triathletes. The Triple Iron ultra-triathletes trained for more hours (P < 0.01) and covered more kilometres (P < 0.01), but speed in running during training was slower compared with the Ironman triathletes (P < 0.01). For Triple Iron ultra-triathletes, percent body fat (P = 0.022), training volume per week (P < 0.0001), and weekly kilometres in both cycling (P < 0.0001) and running (P < 0.0001) were related to race time. For Ironman triathletes, percent body fat (P < 0.0001), circumference of upper arm (P = 0.006), and speed in cycling training (P = 0.012) were associated with total race time. We conclude that both Triple Iron ultra-triathletes and Ironman triathletes appeared to profit from low body fat. Triple Iron ultra-triathletes relied more on training volume in cycling and running, whereas speed in cycling training was related to race time in Ironman triathletes.     

BISp Informationen

BISp-Informationen

BISp Informationen     

Informationen des Bundesinstituts für Sportwissenschaft (BISp)

BISp-Informationen

Informationen des Bundesinstituts für Sportwissenschaft (BISp)     

Rethinking the World Bank Agenda for Chinese Higher Education Reform

This article explores the way in which the World Bank has worked effectively with China in higher education. It investigates whether or not the cooperation between the two has changed in line with their changing relationship. More specifically, it discusses whether the World Bank’s China agenda reflects the reform package of socio-institutional neoliberalism which the World Bank has tended to promote worldwide in the era of the Post-Washington Consensus, and how China’s higher education reform has been influenced by the agenda. The article argues that as China is transferring its role from that of a recipient country to that of a donor country, it is increasingly important to position itself as a global player. Other than mastering the game rules of the international community, China should also expand its influence within and through these major international organizations.     

Vapor-liquid equilibrium simulation of binary and ternary mixtures of CH4, C2H4 and iso-C4H10

The vapor-liquid equilibrium（VLE） properties for the binary and ternary mixtures of CH4,C2H4 and isoC4H10 are of great importance in the recovery of ethylene from mixture containing CH4 and C2H4 with iso-C4H10 as solvent.Hence,Gibbs ensemble Monte Carlo（GEMC） simulations were used to estimate vapor-liquid equilibrium for the binary and ternary mixtures of CH4,C2H4 and iso-C4H10 with the united atom potential NERD model.The selected simulation conditions are based on the experiment in the literature.The results of this work were shown to be in satisfactory agreement with available experimental data and predictions of Peng-Robinson equation of state.The structure of simulated liquid phase is also characterized by radial distribution function（RDF）,which contributes to further understanding of the VLE curve of these systems.RDF is not sensitive to the pressure and temperature range.With the increase of pressure or the decrease of temperature,the molecules tend to gather together.