Formation of embryoid bodies using dielectrophoresis

Embryoid body (EB) formation forms an important step in embryonic stem cell differentiation invivo. In murine embryonic stem cell (mESC) cultures EB formation is inhibited by the inclusion of leukaemic inhibitory factor (LIF) in the medium. Assembly of mESCs into aggregates by positive dielectrophoresis (DEP) in high field regions between interdigitated oppositely castellated electrodes was found to initiate EB formation. Embryoid body formation in aggregates formed with DEP occurred at a more rapid rate-in fact faster compared to conventional methods-in medium without LIF. However, EB formation also occurred in medium in which LIF was present when the cells were aggregated with DEP. The optimum characteristic size for the electrodes for EB formation with DEP was found to be 75-100 microns; aggregates smaller than this tended to merge, whilst aggregates larger than this tended to split to form multiple EBs. Experiments with ESCs in which green fluorescent protein (GFP) production was targeted to the mesodermal gene brachyury indicated that differentiation within embryoid bodies of this size may preferentially occur along the mesoderm lineage. As hematopoietic lineages during normal development derive from mesoderm, the finding points to a possible application of DEP formed EBs in the production of blood-based products from ESCs.     

The problematic relationship between child development theory and teacher preparation

Abstract

This article addresses questions raised in the past decade regarding the role of child development knowledge for teaching and teacher preparation. Missing from the dialogue on this issue have been the voices of the students enrolled in teacher preparation programs. Accordingly, 77 preservice teachers were surveyed at the conclusion of a child development theory course. The results were examined for themes regarding how preservice teachers come to understand the changing nature of child development, the strengths and limitations of generalized theories of development, and the applicability of child development theory to their future practice.     

Effects of implementing flipped classroom elements and dynamic in‐class discussion on student performance

In a Food Processing–Unit Operations course, students learn the basic equipment that comprises unit operations and techniques commonly used in the food industry to prepare, process, and preserve a variety of food products. Due to the complexity of these operations, students frequently struggle with applying food processing principles to predict how unit operations influence the physical, biochemical, sensory, and nutritional properties of foods. This study is designed to evaluate how pre‐class readings and in‐class group activities improve the students’ learning in a Food Processing course. The survey after the exams shows that 48 and 60% of students agree that reading assignments and in‐class group activities help them understand the course material better, respectively. The mean value of exam scores shows that the students in the intervention section (Spring 2018) had significantly higher scores (88.4%) than that of the two previous sections without intervention (77.6%, Spring 2016 and 82.8%, Spring 2017) (P < .05). It is concluded that using a flipped class element like providing reading assignments and quizzes before the lecture can be an effective preparation technique for students as well as providing the instructors with critical insight into the students’ level of understanding before the lecture. This allows the instructor to focus time spent in class on areas in which students struggle the most.     

PROBLEM FINDING: DISCOVERING AND FORMULATING PROBLEMS

Research and theory on problem finding are scarce. This can be ascribed to difficulties in the operationalisation of the concept and to neglect of the importance of this essential link in the problem solving cycle. A similar lacuna, however, is not found in early research in the field of problem solving. In problem solving four components can be distinguished: cognitive (sensitivity to problems and formulating the problem), motivational (effectance motivation and curiosity), emotional (wonder and surprise) and, finally, personality components (tolerance for ambiguity, stimulus reduction and self‐confidence). Selective encoding, combining and comparing can be regarded as essential elements of the cognitive component. With respect to the developmental aspects of problem finding, emphasis is laid on the importance of competence motivation and the quality of attachment as determinants. The educational implications are unequivocal: problem solving and problem finding must have equal positions in curricula. For the time being this means that education will have to pay more attention to problem finding.     

Disruption of chickens’ color aversions when training and test mediums are different

Learning & Behavior - In the present experiments, the conditions under which chickens would avoid colored and flavored substances that they had associated with toxicosis were examined. It was...     

Students’ perception of frequent assessments and its relation to motivation and grades in a statistics course: a pilot study

This pilot study measures university students’ perceptions of graded frequent assessments in an obligatory statistics course using a novel questionnaire. Relations between perceptions of frequent assessments, intrinsic motivation and grades were also investigated. A factor analysis of the questionnaire revealed four factors, which were labelled value, formative function, positive effects and negative effects. The results showed that most students valued graded frequent assessments as a study motivator. A modest number of students experienced positive or negative effects from assessments and grades received. Less than half of the students used the results of frequent assessments in their learning process. The perception of negative effects (lower self-confidence and more stress) negatively mediated the relation between grades and intrinsic motivation. It is argued that communication with students regarding the purpose and benefits of frequent assessments could mitigate these negative effects.     

Evaluating deaf students' readiness to meet the English language and literacy demands of postsecondary educational programs

The purpose of this study was to investigate alternative methods for evaluating deaf students' readiness to meet the English language and literacy demands of postsecondary educational programs. In the first part of the study, scores obtained by a large sample of deaf students on the ACT Assessment (ACT Composite score and scores on the ACT English and Reading tests) were compared to their scores on various measures of English language and literacy skills. In the second part of the study, the performance of a smaller sample of deaf students on the ESL Reading and ESL Grammar/Usage components of COMPASS/ESL was compared to their performance on a set of concurrent measures of English skills. The results of this investigation demonstrate that neither the ACT Assessment nor COMPASS/ESL are appropriate for the full range of deaf students seeking admission to postsecondary educational programs. However, the ACT Assessment is appropriate for deaf students seeking admission to transferable (BS and AAS) degree programs, and the ESL Reading and Grammar/Usage tests appear to be appropriate for deaf students seeking admission to nontransferable (AOS) degree programs. Taken together, the combination of the ACT Assessment and COMPASS/ESL appear able to provide a valid, reliable, and coherent approach to admissions screening assessment for the full range of deaf students seeking admission to postsecondary programs.     

Taste-sickness associations in young rats over varying delays,stimulus, and test conditions

We showed, as had previous investigators, that young rats formed taste-sickness associations that were weaker than those of mature rats; associations were not formed over a delay greater than 45 min, and aversions did not survive a 60-min test session. The difficulty young rats had withholding consumption and their poor sensitivity to taste and sickness contributed to the weak aversions. Choice tests revealed aversions that had apparently extinguished during a no-choice test, and animals that were allowed to mature prior to the first test readily withheld consumption for 60 min. Furthermore, young rats formed an aversion over a delay of 2.5 h when the concentrations of saccharin and lithium chloride were increased. Aversions to the stronger saccharin did not extinguish over two one-bottle tests and were retained for 52 days.     

TOTAL QUALITY MANAGEMENT IN THE NEW UNIVERSITIES: HOW TO APPROACH HUMAN RESOURCE MANAGEMENT

The view taken in this article is that Total Quality Management (TQM) in the new universities (the former Polytechnics) and the development of a so‐called managerialist ideology has led to the inevitable adoption of an approach to Human Resource Management (HRM) policy and practice that is functionalist. The criteria favoured by managerialism represented by TQM is not only inappropriate in higher education, but more importantly, it limits the productive activity of individuals. This limitation occurs to the extent that at the level of the individual academic in higher education organizations, quality control and assurance, that has traditionally been a localized process of self‐ and close‐peer review, has become formalized by externally imposed systems and procedures ‐ a necessary condition under the current funding arrangements for enabling internal quality assurance systems to meet the requirements of external agencies. However, the authors suggest that only a truly professionalizing, soft, individualistic, and user‐focussed collegial culture will provide a sufficient condition for total quality enhancement to become a reality.