中顶庙会的历史叙事与都市村民的文化乡愁

中顶庙是明清时期京师最为著名的碧霞元君庙之一。作为公共记忆的特殊空间,它承载了北京民间社会变迁的经验与脉动,也记录了乡民社会向都市街区转型的历史过程。自2006年以来,中顶庙会以"中顶庙民俗文化节"的名义举办了七届,其仪式过程沿袭了传统的规矩礼法,创造了一个历史与现实、心灵与身体相互融合的文化氛围。这种不断被激活的集体记忆,表面上看是在重温村落历史与追念过往生活,但其实质则是一种社会再生产的情感力量,是都市村民以文化记忆应对现实处境的生存智慧。     

当代社会犯罪化政策实证分析

犯罪化问题是刑事政策的重要课题,即如何界定刑法涉足社会生活的广度和深度的问题。在当代社会,立法者根据现实的政治、经济、文化、科技等条件,对暴力犯罪、职务犯罪、环境犯罪、金融犯罪等制定了不同的犯罪化刑事政策,以此为依据划定犯罪圈,从而维护正常的法制秩序和社会秩序。     

“水产动物疾病诊断学”课程的教学思考

本文旨在介绍医科大学开设“水产动物疾病诊断学”的教学内容、方法和体会,并探讨水产动物疾病诊断学教学过程中存在的问题和拟解决的办法。     

基于培养学生会计执业综合实践能力的独立学院“中级财务会计”教学改革思考

会计人员的执业能力在一定程度上影响其所在单位的经济利益,在财务会计学课程体系中,“中级财务会计学”课程发挥着承上启下的重要作用,如何培养以实践能力为主的复合型应用人才是独立学院中级财务会计教学改革的核心课题。本文借助中级财务会计教学改革的问卷调查,综合分析了现行独立学院中级财务会计教学中存在的问题,并尝试提出了解决这些问题的对策和方法。     

Quadratically convex combination approach to stability of T–S fuzzy systems with time-varying delay

This paper develops a novel stability analysis method for Takagi–Sugeno (T–S) fuzzy systems with time-varying delay. New delay-dependent stability criteria in terms of linear matrix inequalities for time-varying delayed T–S fuzzy systems are derived by the newly proposed augmented Lyapunov–Krasovski (L–K) functional. This functional contains the cross terms of variables and quadratic terms multiplied by a higher degree scalar function. Different from previous results, our derivation applies the idea of second-order convex combination, and the property of quadratic convex function without resorting to the Jensen's inequality. Two numerical examples are provided to verify the effectiveness of the presented results.     

国际专利合作和引用对创新绩效的影响研究

专利合作和专利引用作为重要的知识流动途径,可以帮助创新主体获取外部知识,降低自身创新的投入和风险,对创新活动具有重要意义。本文以16个创新型国家在1981-2007年专利活动为实证对象,通过负二项式模型,在国家层面研究基于国际专利合作和国外专利引用的知识流动对创新绩效的影响,以及吸收能力对各国将外部知识转换为自身产出的影响,实证结果显示,专利合作对创新数量和质量均具有显著促进作用,专利引用对创新数量无显著影响,但对创新质量具有促进作用,吸收能力对创新数量和质量均具有促进作用。     

Microfluidic approaches to rapid and efficient aptamer selection

With their advantages as molecular recognition elements, aptamers have been extensively studied and used for bioanalytical and biomedical applications. However, the process of enrichment and screening of aptamers remains a bottleneck for aptamer development. Recently, microfluidic methods have been increasingly used for rapid and efficient aptamer selection, showing their remarkable advantages over conventional methods. This review briefly introduces aptamers and their advantages. The conventional process of generating aptamers is discussed, followed by the analysis of the key obstacles to efficient aptamer selection. Microfluidic methods for highly efficient enrichment and screening of aptamers are reviewed in detail.     

A highly parallel microfluidic droplet method enabling single-molecule counting for digital enzyme detection

Although digital detection of nucleic acids has been achieved by amplification of single templates in uniform microfluidic droplets and widely used for genetic analysis, droplet-based digital detection of proteins has rarely been reported, largely due to the lack of an efficient target amplification method for protein in droplets. Here, we report a key step towards digital detection of proteins using a highly parallel microfluidic droplet approach for single enzyme molecule detection in picoliter droplets via enzyme catalyzed signal amplification. An integrated microfluidic chip was designed for high throughput uniform droplet generation, monolayer droplet collection, incubation, detection, and release. Single β-galatosidase (β-Gal) molecules and the fluorogenic substrate fluorescein di-β-D-galactopyranoside were injected from two separated inlets to form uniform 20 μm droplets in fluorinated oil at a frequency of 6.6 kHz. About 200 000 droplets were captured as a monolayer in a capture well on-chip for subsequent imaging detection. A series of β-Gal solutions at different concentrations were analyzed at the single-molecule level. With no enzyme present, no droplets were found to fluoresce, while brightly fluorescent droplets were observed under single-enzyme molecule conditions. Droplet fluorescence intensity distribution analysis showed that the distribution of enzyme molecules under single-molecule conditions matched well with theoretical prediction, further proving the feasibility of detecting single enzyme molecules in emulsion droplets. Moreover, the population of fluorescent droplets increased as the β-Gal concentration increased. Based on a digital counting method, the measured concentrations of the enzyme were found to match well with input enzyme concentration, establishing the accuracy of the digital detection method for the quantification of β-Gal enzyme molecules. The capability of highly parallel detection of single enzyme molecules in uniform picoliter droplets paves the way to microdroplet based digital detection of proteins.     

文化因素对培养英语翻译人才的影响——以全球化为背景

论述了培养英语翻译人才的必要性,分析了文化因素对培养英语翻译人才的影响。认为高校在培养翻译人才过程中必须采取有效措施,如提高英语教师自身的文化素养、充分利用课堂教学、充分利用课外活动时间、培养主体文化意识等,以强化对学生中西方文化的教育,达到培养适应社会需求的高素质翻译人才的目的。     

古音学研究与谐声古音学研究述略

文章概述学界在古音学领域研究的历史及现状,梳理并简介了对自宋吴棫以来历代古音学家的研究概况及主要论著.同时,文章对谐声古音研究论著和成果进行了蒐集、整理、考辨和评价,认为归纳谐声与古音学的源流和历史演进,评介各家谐声与古音学思想、理论和方法之优劣及得失,具有很大的学术价值,是古音学研究新的视角和方向.