Anti-angiogenesis effect of generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide on breast cancer in vitro

Objective: To study the effects of the generation 4 polyamidoamine/vascular endothelial growth factor antisense oligodeoxynucleotide (G4PAMAMNEGFASODN) compound on the expressions of vascular endothelial growth factor (VEGF) and its mRNA of breast cancer cells and on the inhibition of vascular endothelial cells. Methods: We examined the morphology of G4PAMAM/VEGFASODN compound and its pH stability, in vitro transfection efficiency and toxicity, and the expressions of VEGF and its mRNA. Methyl thiazolyl tetrazolium assay was used to detect the inhibitory function of the compound on vascular endothelial cells. Results: The compound was about 10 nm in diameter and was homogeneously netlike. From pH 5 to 10, it showed quite a buffered ability. The 48-h transfection rate in the charge ratio of 1:40 was 98.76%, significantly higher than that of the liposome group (P<0.05). None of the transfection products showed obvious toxicity on the cells. The expressions of both VEGF protein and its mRNA after G4PAMAM/VEGFASODN transfection decreased markedly. Conclusion: With a low toxicity, high safety, and high transfection rate, G4PAMAMNEGFASODN could be a promising gene vector. Specifically, it inhibits VEGF gene expression efficiently, laying a basis for further in vivo animal studies.     

信息资源集成与知识集成比较研究

比较分析信息资源集成和知识集成的动因(why)、信息资源集成和知识集成的内涵及特征(what)、信息资源集成和知识集成机制(how),揭示信息资源集成与知识集成的差异和内在联系并得出研究结论:信息资源集成与知识集成相辅相成、相互促进,知识集成是信息资源集成的发展方向。     

大学生心理健康教育的有益形式--图书治疗

图书治疗对大学生心理健康具有独特的教育作用。高校图书馆通过积极的宣传和引导、培养专业馆员和实施分类导读等措施,科学高效地开展图书治疗,能够提高当前大学生心理健康教育的成效。参考文献5。     

环境绩效、环境成本内部化与环境保护:一个研究综述

在经济全球化的同时,环境资源的可持续面临着严峻挑战。本文从梳理环境绩效入手,对环境绩效评价、环境成本内部化以及环境长效保护机制等方面的研究进行综述;并从利益相关者的角度,分析了环境保护各主体间的博弈关系。     

基于TAM的网上购物意向综合模型

回顾了以往对消费者接受网上购物所进行的研究,结合CNNIC调查的我国网上购物发展的实际情氇,提出一个综合的概念模型。该模型以技术接受模型为基础,以消费者个人特性、消费者感知的网站质量特性、消费者感知的商家相关特性三个方面的因素为外源变量,以感知的有用性、感知的易用性和信任作为中介变量,共同影响消费者的网上购物意向。     

从冲突到适应与发展－传统型教师向新型教师的转换

教师是否实现新基础教育改革的最终决定性因素，前提是实现传统型向新型教师的转换，适应并发展新教育。     

我国远程网络教育存在的问题与展望

随着计算机技术和现代教育理论的发展,我国远程网络教育被提上日程,并逐步发展。我国远程网络教育发展起步较晚,但却对中国教育事业的发展具有深远而巨大的意义。目前,我国远程网络教育在发展过程中存在不少问题与不足,本文首先归纳分析了我国远程网络教育的发展现状,进而提出其中存在的问题与不足,在此基础上试图对我国远程网络教育的发展前景进行科学预期与展望。     

Basic fibroblast growth factor alleviates brain injury following global ischemia reperfusion in rabbits

The aim of this study was to explore the protective effect of basic fibroblast growth factor (bFGF) on brain injury following global ischemia reperfusion and its mechanisms. Brain injury following global ischemia was induced by four vessels occlusion and systemic hypotension. Twenty-four rabbits were randomized into three groups: group A, only dissection of vessels; group B, intravenous infusion of normal saline after reperfusion for 6 h; group C, 30 μg/kg bFGF injected intravenously at the onset of reperfusion, then infused with 10 μg/(kg·h) for 6 h. Serum neuron specific enolase (NSE), S-100B, tumor necrosis factor-α(TNF-α), interleukin-1 (IL-1), interleukin-8 (IL-8) were measured before ischemia, 30 min after ischemia, 0.5, 1, 3, 6 h after reperfusion. Brain water content was determined and cerebral histopathological damages were compared. NSE and S-100B were increased 1 h after reperfusion and reached their peaks 6 h after reperfusion, but were much higher in group B than those in group C 3, 6 h after reperfusion. In groups B and C, TNF-a was increased after ischemia and IL-1 and IL-8 were increased significantly 0.5 h after reperfusion, then reached their peaks 6 h, 3 h, 6 h after reperfusion respectively. TNF-a and IL-8 at the time points of 1 h and 3 h and IL-1 at 3 h and 6 h in group C were correspondingly lower than those in group B. These indices in group A were nearly unchanged. There were less severe cerebral histopathological damages in group C compared with group B, but no difference in brain water content. It could be concluded that bFGF alleviates brain injury following global ischemia and reperfusion by down-regulating expression of inflammatory factors and inhibiting their activities.     

遥感影像在煤矿中的解译研究

遥感影像是全国矿产资源开发多目标调查与监测的最基本的信息源,它在矿山开发监测中发挥着重要的作用。本文应用TM、SPOT5、IKONOS等影像,通过野外实地踏勘获取的现场资料及经验,分析煤矿在影像中的特征。从颜色、纹理、地物邻接关系等方面建立不同类型煤矿的遥感解译标志,提出煤矿的信息提取原则,旨在为矿山监测中的人们提供更加合理、准确的日视解译方法。     

我国高职院校“双师型”教师政策的解读与思考

1995年,原国家教委首次提出职业学校要建设“双师型”教师队伍,之后相继出台了15部政策文件．对“双师型”教师队伍的建设做出了规范。但这些政策本身仍有很多问题存在,如“双师型”教师标准界定不清、“双师”教师资格认证体系缺乏等问题。对于存在的问题,应从政策角度确立“双师”教师的标准。进而建立“双师’’教师资格认证体系,建立健全“双师”教师的培训培养体制,完善激励机制。