Managing Masculinities: The Metrosexual Moment

In this essay, I explore the historical moment of metrosexuality in popular culture that, I argue, rather than simply a fad, constituted a logical premise vital to assuaging the “crisis” in masculinity engendered by the phenomenon of commercial masculinity. I trace the ways in which the fleeting trend of metrosexuality was articulated rhetorically in US popular culture in such a way as to rationalise commercial masculinity in targeted, explicit ways. I argue that metrosexuality served a crucial rhetorical function for the reconciliation of commercial masculinity with normative masculinity by organising homosociality in strategic ways. Accordingly, I suggest that apparently transient popular cultural trends might best be understood in terms of their location in—and strategic rhetorical function for—broader cultural discourses.     

Reinventing Privilege: The New (Gay) Man in Contemporary Popular Media

In recent years, the gay man/heterosexual woman couple configuration has become a genre unto itself in mediated popular culture, resulting in unprecedented mainstream visibility for gay men. Major mainstream films, such as My Best Friend's Wedding, Object of My Affection, and The Next Best Thing, showcase this combination as their centerpiece, as does the highly rated prime-time network situation comedy, Will & Grace. In this essay, I assess this particular performance of gay identity in order to discern what qualities render it – as presented in this configuration – not only acceptable but popular, given the heteronormative sensibilities that characterize the mainstream audience to which it is directed. I argue that, in these texts, homosexuality is not only recoded and normalized in these representations as consistent with privileged male heterosexuality but is articulated as extending heterosexual male privilege. In so doing, blatant sexism is reinvented and legitimized, and gay male identity simultaneously is defined by and renormalizes heteronormativity.     

Public apprehension of emerging infectious diseases: are changes afoot?

Using social representations theory this paper casts light on the pattern of content that characterises the public response to emerging and re-emerging infectious diseases (EID). The pattern is: distancing the disease from the self/ one's in-groups; blame of particular entities for the disease's origin and/or spread; and stigmatisation of those who have contracted it and/or who are represented as having intensified its spread. This pattern is not unique to EID but extends to many risks, making EID fruitful events for understanding public apprehension of potential dangers. This process may be driven by worry, fear and anxiety since when levels of these are low, as has arguably been the case with the 2009/10 "Swine Flu" pandemic, the pattern transforms. The distancing-blame-stigma pattern may also be transformed by growing reflexivity, a feature of late modern societies, as well as material features of the epidemic and "EID fatigue".     

Microfluidic device for generating a stepwise concentration gradient on a microwell slide for cell analysis

Understanding biomolecular gradients and their role in biological processes is essential for fully comprehending the underlying mechanisms of cells in living tissue. Conventional in vitro gradient-generating methods are unpredictable and difficult to characterize, owing to temporal and spatial fluctuations. The field of microfluidics enables complex user-defined gradients to be generated based on a detailed understanding of fluidic behavior at the μm-scale. By using microfluidic gradients created by flow, it is possible to develop rapid and dynamic stepwise concentration gradients. However, cells exposed to stepwise gradients can be perturbed by signals from neighboring cells exposed to another concentration. Hence, there is a need for a device that generates a stepwise gradient at discrete and isolated locations. Here, we present a microfluidic device for generating a stepwise concentration gradient, which utilizes a microwell slide''s pre-defined compartmentalized structure to physically separate different reagent concentrations. The gradient was generated due to flow resistance in the microchannel configuration of the device, which was designed using hydraulic analogy and theoretically verified by computational fluidic dynamics simulations. The device had two reagent channels and two dilutant channels, leading to eight chambers, each containing 4 microwells. A dose-dependency assay was performed using bovine aortic endothelial cells treated with saponin. High reproducibility between experiments was confirmed by evaluating the number of living cells in a live-dead assay. Our device generates a fully mixed fluid profile using a simple microchannel configuration and could be used in various gradient studies, e.g., screening for cytostatics or antibiotics.     

Perceiving "outside the box" occurs early in development: evidence for boundary extension in three- to seven-month-old infants

This investigation examined whether infants display boundary extension-a tendency to remember more of a visual scene than was presented. Three- to 7-month-olds were familiarized with a photograph of a visual scene, and tested with wide-angle versus close-up views of the scene. Infants preferred the close-up, indicating that they perceived the wide angle (the one consistent with boundary extension) as more familiar. Converging experiments showed that: (a) infants did not spontaneously prefer the close-up, (b) adults did not judge the wide angle to be more similar to the familiarization stimulus, and (c) infants spontaneously preferred the close-up when the photographs depicted outline objects without backgrounds. The findings suggest that infants anticipate information that lies beyond the borders of a scene view.     

The influence of task and gender on search and evaluation behavior using Google

To improve search engine effectiveness, we have observed an increased interest in gathering additional feedback about users’ information needs that goes beyond the queries they type in. Adaptive search engines use explicit and implicit feedback indicators to model users or search tasks. In order to create appropriate models, it is essential to understand how users interact with search engines, including the determining factors of their actions. Using eye tracking, we extend this understanding by analyzing the sequences and patterns with which users evaluate query result returned to them when using Google. We find that the query result abstracts are viewed in the order of their ranking in only about one fifth of the cases, and only an average of about three abstracts per result page are viewed at all. We also compare search behavior variability with respect to different classes of users and different classes of search tasks to reveal whether user models or task models may be greater predictors of behavior. We discover that gender and task significantly influence different kinds of search behaviors discussed here. The results are suggestive of improvements to query-based search interface designs with respect to both their use of space and workflow.     

Metabolite profiling of microfluidic cell culture conditions for droplet based screening

We investigate the impact of droplet culture conditions on cell metabolic state by determining key metabolite concentrations in S. cerevisiae cultures in different microfluidic droplet culture formats. Control of culture conditions is critical for single cell/clone screening in droplets, such as directed evolution of yeast, as cell metabolic state directly affects production yields from cell factories. Here, we analyze glucose, pyruvate, ethanol, and glycerol, central metabolites in yeast glucose dissimilation to establish culture formats for screening of respiring as well as fermenting yeast. Metabolite profiling provides a more nuanced estimate of cell state compared to proliferation studies alone. We show that the choice of droplet incubation format impacts cell proliferation and metabolite production. The standard syringe incubation of droplets exhibited metabolite profiles similar to oxygen limited cultures, whereas the metabolite profiles of cells cultured in the alternative wide tube droplet incubation format resemble those from aerobic culture. Furthermore, we demonstrate retained droplet stability and size in the new better oxygenated droplet incubation format.