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In this paper, we present a microfluidic platform for the continuous generation of stable, monodisperse lipid vesicles 20–110 μm in diameter. Our approach utilizes a microfluidic flow-focusing droplet generation design to control the vesicle size by altering the system’s fluid flow rates to generate vesicles with narrow size distribution. Double emulsions are first produced in consecutive flow-focusing channel geometries and lipid membranes are then formed through a controlled solvent extraction process. Since no strong solvents are used in the process, our method allows for the safe encapsulation and manipulation of an assortment of biological entities, including cells, proteins, and nucleic acids. The vesicles generated by this method are stable and have a shelf life of at least 3 months. Here, we demonstrate the cell-free in vitro synthesis of proteins within lipid vesicles as an initial step towards the development of an artificial cell. 相似文献
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María Luisa Sanz de Acedo Lizarraga María Teresa Sanz de Acedo Baquedano María Soria Oliver 《Educational studies》2010,36(3):329-340
The objective of this research was to examine the effects of the instruction method “thinking actively in an academic context (TAAC)” on thinking skills, creativity, self‐regulation and academic achievement. The design was pre‐test–intervention–post‐test with control group. The sample included 46 participants (aged 16 to 18 years), 24 experimental and 22 controls. Before and after application of the method, six instruments were used to measure thinking skills and academic achievement. The method, divided into eight stages, was followed in each didactic unit during an academic course. The method allows teaching the thinking skills, the creativity and the self‐regulation simultaneously with the syllabus content. The results showed that greater changes were obtained with the new method of instruction in all the dependent variables. Relevant scientific and educational implications are drawn from the study. 相似文献