Kinetic study of low density lipoprotein oxidation by copper

Oxidation of Low Density Lipoprotein (LDL) is regarded as a key event in the development of atherosclerosis. The aim of this study was to investigate effect of various copper concentrations on LDL oxidation kinetic profile as a mechanism in atherosclerosis process. LDL was isolated from plasma and its oxidation with copper was investigated by monitoring the formation of conjugated dienes. Based on time course of the formation of conjugated diene was observed at concentrations of 0.5 to 10 μM copper, represented the conventional kinetics of LDL oxidation with an inhibition period followed by a propagation phase. In contrast, at concentrations of 20 to 50 μM copper, LDL oxidation proceeded after a negligibly short lag-time followed by a distinct propagation phase. At lower copper concentrations of about 0.5 μM, LDL oxidation can be combined in 4 consecutive oxidation phase. The increasing copper concentration (to 10 μM) lowered the first propagation and shortened the seconded inhibition period until they melted into one apparent kinetic phase. But in copper concentrations of about 20 to 50 μM, increasing copper concentration increased the first propagation and the second inhibition but lowered the second propagation phase. The results of this investigation on the copper dependence of the oxidation kinetics suggest that LDL contains two different copper binding sites. Copper bound to the low affinity binding sites with molar ratio of 200 to 500 of copper / LDL. These ions bound to the high affinity binding sites with molar ratio of copper / LDL of 5 to 100.     

Chip morphology predictions while machining hardened tool steel using finite element and smoothed particles hydrodynamics methods

Chip morphology predictions in metal cutting have always been challenging because of the complexity of the various multiphysical phenomena that occur across the tool-chip interface. An accurate prediction of chip morphology is a key factor in the assessment of a particular machining operation with regard to both tool performance and workpiece quality. Although finite element (FE) models are being developed over the last two decades, their capabilities in modeling correct material flow around the tool tip with shear localization are very limited. FE models with an arbitrary Lagrangian Eulerian (ALE) approach are able to simulate correct material flow around the tool tip. However, these models are unable to predict any shear localization based on material flow criteria. On the other hand, FE models with a Lagrangian formulation can simulate shear localization in the chip segments; they need to make use of a mesh-based chip separation criterion that significantly affects material flow around the tool tip. In this study a mesh-free method viz. smoothed particles hydrodynamics (SPH) is implemented to simulate shear localization in the chip while machining hardened steel. Unlike other SPH models developed by some researchers, this model is based on a renormalized formulation that can consider frictional stresses along the tool-chip interface giving a realistic chip shape and material flow. SPH models with different cutting parameters are compared with the traditional FE models and it has been found that the SPH models are good for predicting shear localized chips and do not need any geometric or mesh-based chip separation criteria.     

Relationship between social capital and lifestyle among older adults

ABSTRACT

Objective: This study was designed with the purpose of determining the relationship between social capital and lifestyle and their sub-variables among older adults.

Methods: This is a cross-sectional study using interviews that was conducted in 2017. This study was performed with randomly selected older people over 60 years of age in an urban area of Iran. Data were collected using a questionnaire in 3-month duration. The relationship between the study variables (lifestyle and social capital) was assessed through correlation analysis (in the case of non control of the underlying variables) and general linear modeling (in the case of control of the underlying variables). Data were analyzed using SPSS v.24 software at a 0.05 significant level.

Results: A total of 512 questionnaires were filed out. According to the Pearson correlation test, all social capital variables were associated with lifestyle components. However, in the general linear model test, feeling of trust and safety (p < 0.001), value of life (p = 0.030), and social participation (p = 0.004) had a significant relationship with the lifestyle.

Conclusions: The results indicated that social capital and lifestyle in the older adults were interconnected. Therefore, there is a need to improve social capital factors, especially social participation, feeling of trust and safety, and value of life, which is in-line with the strategies of achieving healthy older population. The results can be used to learn how to increase lifestyle in older adults.     

The role of teacher’s corrective feedback in improving Iranian EFL learners’ writing accuracy over time: is learner’s mother tongue relevant?

The purpose of the present study was to investigate the impact of feedback on writing accuracy over time and examine the relevance of the students’ mother tongue to the feedback effect. To this end, the study compared two groups of Iranian English majors (N = 56) over a period of four months: one with indirect grammar feedback and the other with no grammar feedback whatsoever. The results of the study did not show a significant effect for the teachers’ feedback. However, the results showed a main effect for practice and the interaction of practice and feedback as a result of which both groups improved their writing accuracy over the course of one semester, with the feedback group making more improvement. The results also showed significant differences between the two groups in reducing their errors in various grammatical categories. The study further revealed that the success ratio of error reduction in each category was highly sensitive to cross-linguistic differences. The students’ views, elicited after the study, support the findings obtained from the analysis of the quantitative data.     

Incorporating affordability,efficiency, and quality in the ICT development index: Implications for index building and ICT policymaking

Abstract

International Telecommunication Union’s ICT Development Index and related measures of a country’s ICT development maturity suffer from several limitations, including subjective estimation of the weights of individual indicators and sub-indices, use of inappropriate quantitative models, specification bias arising from the exclusion of potential predictors from the estimation models, and a failure to capture the disparities among different groups of countries. To overcome these problems and provide a more reliable measure of ICT development, this study develops the Modified ICT Maturity Level Index using the 2015 data of 166 countries. This index adds affordability, efficiency, and quality to the existing sub-indices of access, use, and skills. Sub-index and indicator weights are determined in an outcome-orientated way using Partial Least Squares Structural Equation Modeling. We find that affordability, quality, and efficiency significantly explain the variation in the level of maturity of ICT development in addition to the previously used dimensions of International Telecommunication Union’s ICT Development Index and modified ICT Development Index (mIDI) developed by Gerpott and Ahmadi, and that their explanatory power differ by a country’s level of economic development. The new index produces significantly different country rankings. This has important implications for ICT policy priorities and provides a measure of ICT development maturity less prone to the innocent or intentional distortion of such policy priorities.     

Modified particle swarm optimization for optimum design of spread footing and retaining wall

This paper deals with the economically optimized design and sensitivity of two of the most widely used systems in geotechnical engineering: spread footing and retaining wall. Several recent advanced optimization methods have been developed, but very few of these methods have been applied to geotechnical problems. The current research develops a modified particle swarm optimization (MPSO) approach to obtain the optimum design of spread footing and retaining wall. The algorithm handles the problem-specific constraints using a penalty function approach. The optimization procedure controls all geotechnical and structural design constraints while reducing the overall cost of the structures. To verify the effectiveness and robustness of the proposed algorithm, three case studies of spread footing and retaining wall are illustrated. Comparison of the results of the present method, standard PSO, and other selected methods employed in previous studies shows the reliability and accuracy of the algorithm. Moreover, the parametric performance is investigated in order to examine the effect of relevant variables on the optimum design of the footing and the retaining structure utilizing the proposed method.     

A hybrid dielectrophoretic system for trapping of microorganisms from water

Assessment of the microbial safety of water resources is among the most critical issues in global water safety. As the current detection methods have limitations such as high cost and long process time, new detection techniques have transpired among which microfluidics is the most attractive alternative. Here, we show a novel hybrid dielectrophoretic (DEP) system to separate and detect two common waterborne pathogens, Escherichia coli (E. coli), a bacterium, and Cryptosporidium parvum (C. parvum), a protozoan parasite, from water. The hybrid DEP system integrates a chemical surface coating with a microfluidic device containing inter-digitated microelectrodes to impart positive dielectrophoresis for enhanced trapping of the cells. Trimethoxy(3,3,3-trifluoropropyl) silane, (3-aminopropyl)triethoxysilane, and polydiallyl dimethyl ammonium chloride (p-DADMAC) were used as surface coatings. Static cell adhesion tests showed that among these coatings, the p-DADMAC-coated glass surface provided the most effective cell adhesion for both the pathogens. This was attributed to the positively charged p-DADMAC-coated surface interacting electrostatically with the negatively charged cells suspended in water leading to increased cell trapping efficiency. The trapping efficiency of E. coli and C. parvum increased from 29.0% and 61.3% in an uncoated DEP system to 51.9% and 82.2% in the hybrid DEP system, respectively. The hybrid system improved the cell trapping by encouraging the formation of cell pearl-chaining. The increment in trapping efficiency in the hybrid DEP system was achieved at an optimal frequency of 1 MHz and voltage of 2.5 V_pp for C. parvum and 2 V_pp for E. coli, the latter is lower than 2.5 V_pp and 7 V_pp, respectively, utilized for obtaining similar efficiency in an uncoated DEP system.     

Right ventricular function in elite male athletes meeting the structural echocardiographic task force criteria for arrhythmogenic right ventricular cardiomyopathy

Athlete pre-participation screening is focused on detecting pathological conditions like arrhythmogenic right ventricular cardiomyopathy (ARVC). The diagnosis of ARVC is established by applying the revised 2010 ARVC Task Force Criteria (TFC) that assesses RV structure and function. Some athletes may meet structural TFC without having ARVC but we do not know the consequences for RV function. This study compared RV structural and functional indices in male athletes that meet the structural TFC (MTFC) for ARVC and those that do not (NMTFC). We recruited 214 male elite athletes. All participants underwent 2D, Doppler, tissue Doppler and strain (ε) echocardiography with a focused and comprehensive assessment of the right heart. Athletes were grouped on RV structural data: MTFC n = 34; NMTFC n = 180. Functional data were compared between groups. By selection, MTFC had larger absolute and scaled RV outflow tract (RVOT) diameter compared to NMTFC (P ?0.05) but these athletes did not develop a proportional increase in the RV inflow dimensions. There was no difference in global conventional RV systolic function between both groups however, there was significantly lower global RV ε in athletes that MTFC which can be explained, in part, by the RVOT dimension.