Polymorphonuclear leukocyte mediated oxidative inactivation of alpha-1-proteinase inhibitor: Modulation by nitric oxide

Alpha-1-proteinase inhibitor activity was studied in presence of resting and activated polymorphonuclear leucocytes. Four different agonists; phorbol myristic acetate, N-formyl-methionyl-leucyl-phenylalanine, opsonised zymosan and arachidonic acid decreased the inhibitor activity by 23.3%, 20%, 12% and 16.6^ respectively. The inhibitor activity was protected by using various free radical scavengers. Catalase and superoxide dismutase both restored activity by about 18%, mannitol by 13% and sodium azide by 17.3%. The inhibitor activity was also protected significantly by pretreatment of polymorphs with L-Arg, a precursor of nitric oxide, before activation. L-Arg was also observed to suppress the generation of superoxide and hydroxyl radical appreciably. The nitric oxide synthase inhibitor, aminoguanidine drastically inhibited the nitrite release and reversed the protection offered by L-Arg to the inhibitor activity. Our results indicate a multifactorial nature of the inactivation process, the culprit species being superoxide, hydrogen peroxide, hydroxyl radical and hypohalides. Nitric oxide seems to scavenge the superoxide radical directly after its formation rather than inhibiting its generation by NADPH oxidase as was believed earlier.     

An evaluation of traditional admission standards in predicting Kuwait University students' academic performance

The purpose of this study was to evaluate the usefulness of the traditional admission standards utilized by Kuwait University in predicting students' academic performance. It was found that the established practice of using students' score in standardized secondary school examinations and branch of study (i.e. sciences or arts emphasis) to be highly predictive of their college cumulative grade point average. Moreover, it was established that students' secondary school scores reflect intellective as well as the non-intellective factors pertaining to students' background.     

Investigating the potential of using acoustic frequency on the degradation of linear alkylbenzen sulfonates from aqueous solution

The effectiveness of using acoustical (sonochemical) reactor for degradation of linear alkylbenzen sulfonate (LAS) from aqueous solution was investigated. LASs are anionic surfactants,found in relatively high amounts in domestic and industrial wastewaters. In this study,experiments on LAS solution were performed using methylene blue active substances (MBAS) method. The effectiveness of acoustical processor reactor for LAS degradation is evaluated with emphasis on the effect of treatment time and initial LAS concentration. Experiments were performed at initial concentrations of 0.2,0.5,0.8 and 1.0 mg/L,acoustic fre-quency of 130 kHz,applied power of 500 W and temperature of 18 °C～20 °C. At the conditions involved,LAS degradation was found to increase with increasing sonochemical time. In addition,as the concentration increased,the LAS degradation rate de-creased in the acoustical processor reactor.     

Induction of alternative fate other than default neuronal fate of embryonic stem cells in a membrane-based two-chambered microbioreactor by cell-secreted BMP4

Cell-secreted soluble factor signaling in a diffusion dominant microenvironment plays an important role on early stage differentiation of pluripotent stem cells invivo. In this study, we utilized a membrane-based two-chambered microbioreactor (MB) to differentiate mouse embryonic stem cells (mESCs) in a diffusion dominant microenvironment of the top chamber while providing enough nutrient through the bottom chamber. Speculating that accumulated FGF4 in the small top chamber will augment neuronal differentiation in the MB culture, we first differentiated mESCs for 8 days by using a chemically optimized culture medium for neuronal induction. However, comparison of cellular morphology and expression of neuronal markers in the MB with that in the 6-well plate (6WP) indicated relatively lower neuronal differentiation in the MB culture. Therefore, to investigate whether microenvironment in the MB facilitates non-neuronal differentiation, we differentiated mESCs for 8 days by using chemically defined basal medium. In this case, differentiated cell morphology differed markedly between the MB and 6WP cultures: epithelial sheet-like morphology in the MB, whereas rosette morphology in the 6WP. Expression of markers from the three germ layers indicated lower neuronal but higher meso- and endo-dermal differentiation of mESCs in the MB than the 6WP culture. Moreover, among various cell-secreted soluble factors, BMP4 expression was remarkably upregulated in the MB culture. Inhibition of BMP4 signaling demonstrated that enhanced effect of upregulated BMP4 was responsible for the prominent meso- and endo-dermal differentiation in the MB. However, in the 6WP, downregulated BMP4 had a minimal influence on the differentiation behavior. Our study demonstrated utilization of a microbioreactor to modulate the effect of cell-secreted soluble factors by autoregulation and thereby inducing alternative self-capability of mESCs. Understanding and implementation of autoregulation of soluble factors similar to this study will lead to the development of robust culture systems to control ESC behavior.     

Feedback about more accurate versus less accurate trials: differential effects on self-confidence and activation

One purpose of the present study was to examine whether self-confidence or anxiety would be differentially affected byfeedback from more accurate rather than less accurate trials. The second purpose was to determine whether arousal variations (activation) would predict performance. On day 1, participants performed a golf putting task under one of two conditions: one group received feedback on the most accurate trials, whereas another group received feedback on the least accurate trials. On day 2, participants completed an anxiety questionnaire and performed a retention test. Shin conductance level, as a measure of arousal, was determined. The results indicated that feedback about more accurate trials resulted in more effective learning as well as increased self-confidence. Also, activation was a predictor of performance.     

The interfaculty graduate environmental sciences program of the American University of Beirut: an ESD initiative in the Arab World

The American University of Beirut’s Interfaculty Graduate Environmental Sciences Program was launched in 1997 as a means of addressing salient issues on the environment and development in Lebanon and the Arab World using an interdisciplinary approach. The programme adopts a student-centred learning approach and aims to develop critical and systems thinking skills to produce socially and environmentally conscious leaders and agents of change in the Arab World. In this paper, we provide an evaluation of the programme’s ESD dimensions using the criteria of interdisciplinarity, local relevance and competence-based learning. This is followed by a critical analysis of the programme’s potential for use as a model in the Arab World. We find that, while the model may be useful in providing inspiration and a good practice case, its transfer “as is” to institutions of higher education in the Arab World or even in Lebanon is unlikely, and perhaps undesirable, in view of the inherently diverse nature of Arab universities.     

Chemical composition and antioxidant activity of certain <Emphasis Type="Italic">Morus</Emphasis> species

In the present work, the fruits of four Morus species, namely Morus alba (white mulberry), Morus nigra (black mulberry), Morus laevigata (large white fruit), and Morus laevigata (large black fruit), were analyzed for proximate composition, essential minerals, and antioxidant potentials. For this purpose, the ripe fruits were collected from the northern regions of Pakistan. The major nutritional components (moisture, ash, lipids, proteins, fibres, carbohydrates, and total sugar) were found to be in the suitable range along with good computed energy. Total dry weight, pH, and titratable acidity (percent citric acid) were (17.60±1.94)–(21.97±2.34) mg/100 g, (3.20±0.07)–(4.78±0.15), and (0.84±0.40)%–(2.00±0.08)%, respectively. Low riboflavin (vitamin B₂) and niacin (vitamin B₃) contents were recorded in all the fruits, while ascorbic acid (vitamin C) was in the range from (15.20±1.25) to (17.03±1.71) mg/100 g fresh weight (FW). The mulberry fruits were rich with regard to the total phenol and alkaloid contents, having values of (880±7.20)–(1650±12.25) mg/100 g FW and (390±3.22)–(660±5.25) mg/100 g FW, respectively. Sufficient quantities of essential macro-(K, Ca, Mg, and Na) and micro-(Fe, Zn, and Ni) elements were found in all the fruits. K was the predominant element with concentration ranging from (1270±9.36) to (1731±11.50) mg/100 g, while Ca, Na, and Mg contents were (440±3.21)–(576±7.37), (260±3.86)–(280±3.50), and (240±3.51)–(360±4.20) mg/100 g, respectivly. The decreasing order of micro-minerals was Fe>Zn>Ni. The radical scavenging activity of methanolic extract of fruits was concentration-dependent and showed a correlation with total phenolic constituents of the respective fruits. Based on the results obtained, mulberry fruits were found to serve as a potential source of food diet and natural antioxidants.     

Effective Integration of ICT in Jordanian Schools: An Analysis of Pedagogical and Contextual Impediments in the Science Classroom

A series of interviews and classroom observations were conducted with a group of in-service science teachers, students, school principal, and computer lab supervisors, from a “Discovery” female school in Jordan to assess their utilization of information and communication technology (ICT) in teaching science. The study also intended to determine how these participants were using ICT and if they had any internal and external impediments in the way of the effective integration of ICT in the teaching of science. Results showed that some participants were using ICT creatively in their science teaching. However, despite considerable political pressure to increase ICT use in the classroom, most expressed frustration at the lack of ICT tools, support from the school, from the Ministry of Education, and from the surrounding community. The article proposes possible resolutions to help these participants overcome their impediments. Some of the suggested resolutions for the internal impediments include involving teachers in preparing the school’s time-table, equipping the school with more ICT tools and offering more training courses for teachers. However, the suggested resolutions for the external impediments involve (1) The Ministry of Education to rethink the administration of board examinations, (2) The school to sacrifice scoring higher in board examinations for preparing more creative and more versatile students’ perspectives.     

Online learning of an interval type-2 TSK fuzzy logic controller for nonlinear systems

In this study, an adaptive interval type-2 Takagi-Sugeno-Kang fuzzy logic controller based on reinforcement learning (AIT2-TSK-FLC-RL) is proposed. The proposed controller consists of an actor, a critic and a reward signal. The actor is represented by the IT2-TSK-FLC in which the antecedents and the consequents are interval type-2 fuzzy sets (IT2FSs) and type-1 fuzzy sets (T1FSs), respectively, which are named A2-C1. The critic is represented by a neural network, which approximates the optimal guaranteed cost in the control design to ensure the system stability for all admissible uncertainties and noise. The use of a reward signal to formalize the idea of a goal is one of the most distinctive features of RL. Thus, the proposed controller evolves in time as a result of the online learning algorithm. The parameters of the proposed controller are learned online based on the Lyapunov theorem to guarantee the stability, overcome the shortcomings of the gradient descent, such as the local minima and instability, and determine the learning rate of the IT2-TSK-FLC controller. Furthermore, the critic stability is discussed for determining the optimal learning rate. The proposed controller is applied to uncertain nonlinear systems to show its robustness in reducing the effect of system uncertainties and external disturbances and is compared to other controllers.