Efficient sample preparation in immuno-matrix-assisted laser desorption/ionization mass spectrometry using acoustic trapping

Acoustic trapping of minute bead amounts against fluid flow allows for easy automation of multiple assay steps, using a convenient aspirate/dispense format. Here, a method based on acoustic trapping that allows sample preparation for immuno-matrix-assisted laser desorption/ionization mass spectrometry using only half a million 2.8 μm antibody covered beads is presented. The acoustic trapping is done in 200 × 2000 μm² glass capillaries and provides highly efficient binding and washing conditions, as shown by complete removal of detergents and sample processing times of 5-10 min. The versatility of the method is demonstrated using an antibody against Angiotensin I (Ang I), a peptide hormone involved in hypotension. Using this model system, the acoustic trapping was efficient in enriching Angiotensin at 400 pM spiked in plasma samples.     

Students’ meaning making in science: solving energy resource problems in virtual worlds combined with spreadsheets to develop graphs

The aim of this study is to scrutinize the characteristics of conceptual meaning making when students engage with virtual worlds in combination with a spreadsheet with the aim to develop graphs. We study how these tools and the representations they contain or enable students to construct serve to influence their understanding of energy resource consumption. The data were gathered in 1st grade upper-secondary science classes and they constitute the basis for the interaction analysis of students?? meaning making with representations. Our analyses demonstrate the difficulties involved in developing students?? orientation toward more conceptual orientations to representations of the knowledge domain. Virtual worlds do not in themselves represent a solution to this problem.     

Computer-based 3D simulation: a study of communication practices in a trauma team performing patient examination and diagnostic work

Diagnostic work in trauma teams is critical for the patient’s condition and for the possibility of survival. It is a difficult situation to train due to the inherently unpredictable and time-critical practice when an injured patient presents in the Emergency Room (ER). Different types of simulations have been developed for specialized training of specific skills and on contribution to teamwork, but there are hardly any studies reporting on opportunities to train realistic, multidisciplinary collaboration and communicative skills in such time-critical settings. In this article we report on a collaboration-oriented simulation where a trauma team performs diagnostic work: examining a patient in an ER at a hospital. The setting studied is arranged as a design experiment and video data constitute the basis for our interaction analysis. Our main finding is that highly-specialized simulations are useful as an arena for communication training among trauma team members. Doctors and nurses manage to make the simulated representations relevant in their talk, share medical observations and examinations, and consecutively include these findings into their collaborative diagnostic trajectory.     

Giving a voice to traumatized youth—Experiences with Trauma-Focused Cognitive Behavioral Therapy

The efficacy of Trauma-Focused Cognitive Behavioral Therapy (TF-CBT) has been shown in several randomized controlled trials. However, to our knowledge no one has studied the TF-CBT model from a user's perspective. The objective of this study was to explore traumatized youths’ experiences of receiving TF-CBT. Thirty youths between 11 and 17 years old (M = 15, SD = 1.8) were interviewed using a semi-structured interview guide after they had received TF-CBT as part of an effectiveness trial. The interviews were analyzed according to thematic analysis. The youths’ responses were grouped into four themes: (1) expectations, (2) experiences of talking to the therapist and sharing information, (3) experiences of trauma narrative work, and (4) experiences of change and change processes. Findings showed how an initial fear of talking about traumatic events and not knowing what to expect from therapy was reduced when the youth experienced the therapist as empathetic and knowledgeable. Talking to the therapist was experienced as positive because of the therapist's expertise, neutrality, empathy, and confidentiality. Talking about the trauma was perceived as difficult but also as most helpful. Learning skills for reducing stress was also perceived as helpful. Important change processes were described as resuming normal functioning and getting “back on track,”, or as acquiring new perspectives and “moving forward.” Because TF-CBT is recommended as a first line treatment for traumatized youth and treating posttraumatic stress may entail special challenges, understanding more about how youths experience this mode of treatment contributes to our knowledge base and may help us tailor interventions.     

On the determinants of employment-related organised education and informal learning

This paper analyses the distribution of employment-related organised education and informal learning in the Canadian workforce. The paper draws on a large-scale survey, the Changing Nature of Work and Lifelong Learning (WALL), which was based on structured and standardised telephone interviews with a representative sample of 5783 Canadian members of the employed labour force. In exploring the determinants facilitating employment-related informal learning, three analytical categories of factors derived from previous research on learning participation were used: individual-level factors, job characteristics and workplace environment. The analyses focus on differences in individuals, jobs and workplace characteristics among adult workers who acquired or improved their job-related skills through different training pathways. In addition, analyses were performed to compare the extent to which these factors differ in their influence on learning decisions among workers who combine both organised education and informal learning and those who receive only informal learning. The results indicate that important predictors of participation in employment-related organised education and informal learning are age, educational attainment, learning skills, occupational class, education-job relation, degree of autonomy, degree of labour intensity, principal area of production and organisation size.     

How Will We Understand What We Teach? - Primary Student Teachers’ Perceptions of their Development of Knowledge and Attitudes Towards Physics

The research outlined in this paper investigated how student teachers perceived the development of their knowledge and attitudes towards physics through video recorded practical workshops based on experiments and subsequent group discussions. During an 8-week physics course, 40 primary science student teachers worked in groups of 13–14 on practical experiments and problem-solving skills in physics. The student teachers were video recorded in order to follow their activities and discussions during the experiments. In connection with every workshop, the student teachers participated in a seminar conducted by their physics teachers and a primary science teacher; they watched the video recording in order to reflect on their activities and how they communicated their conceptions in their group. After the 8 weeks of coursework a questionnaire including a storyline was used to elicit the student teachers’ perceptions of their development of subject matter knowledge from the beginning to the end of the course. Finally, five participants were interviewed after the course. The results provided insight into how aspects such as self-confidence and the meaningfulness of knowledge for primary teaching were perceived as important factors for the primary science student teachers’ development of subject matter knowledge as well as a positive attitude towards physics.     

Selective cell capture and analysis using shallow antibody-coated microchannels

Demand for analysis of rare cells such as circulating tumor cells in blood at the single molecule level has recently grown. For this purpose, several cell separation methods based on antibody-coated micropillars have been developed (e.g., Nagrath et al., Nature 450, 1235–1239 (2007)). However, it is difficult to ensure capture of targeted cells by these methods because capture depends on the probability of cell-micropillar collisions. We developed a new structure that actively exploits cellular flexibility for more efficient capture of a small number of cells in a target area. The depth of the sandwiching channel was slightly smaller than the diameter of the cells to ensure contact with the channel wall. For cell selection, we used anti-epithelial cell adhesion molecule antibodies, which specifically bind epithelial cells. First, we demonstrated cell capture with human promyelocytic leukemia (HL-60) cells, which are relatively homogeneous in size; in situ single molecule analysis was verified by our rolling circle amplification (RCA) method. Then, we used breast cancer cells (SK-BR-3) in blood, and demonstrated selective capture and cancer marker (HER2) detection by RCA. Cell capture by antibody-coated microchannels was greater than with negative control cells (RPMI-1788 lymphocytes) and non-coated microchannels. This system can be used to analyze small numbers of target cells in large quantities of mixed samples.     

A new method for recording the temporal pattern of stride during treadmill running

The aim of this study was to investigate the reliability of a new infrared light based method (IR₄₀) for recording temporal stride patterns during treadmill running. The IR₄₀ device, emitting a tight web of 40 infrared light beams 10 mm above the treadmill running surface, was compared to a previously validated electro-pneumatic contact shoe (CS) method while nine well-trained athletes ran at 2.8, 3.3, 3.9, 4.4, 5.0, and 5.6 m s⁻¹. Disconnection and reconnection of the IR beams marked the stance phase. The sampling rate was 500 Hz for both methods. The stance phase duration was on average 11.5 (±8.4) ms longer with the IR₄₀ than with the CS depending on earlier touch down (8.3 ± 6.2 ms) and delayed toe off (3.2 ± 5.3 ms) registrations. Significantly different stance phases were recorded between all velocities and for both methods. Thus, despite the fact that the IR₄₀ systematically measured a somewhat longer stance phase duration than CS, the IR₄₀ is nonetheless useful for temporal stride analysis during treadmill running.     

The stroke has only a minor influence on direction consistency in golf putting among elite players

In the golf instructional literature, the putting stroke is typically given higher priority than green reading and aiming. The main purpose of this study was to assess the importance of the putting stroke for direction consistency in golf putting. Kinematic stroke parameters were recorded from 71 elite golf players (mean handicap = 1.8, s = 4.2) on 1301 putts from about 4 m. Of the different factors deciding stroke direction consistency, face angle was found to be the most important (80%), followed by putter path (17%) and impact point (3%). This suggests that improvements in consistency of putter path and impact point will have very little effect on overall putting direction consistency and should not be prioritized in the training of elite players. In addition, mean stroke direction variability for an elite player (European Tour) was found to be 0.39 degrees, which is good enough to hole about 95% of all 4-m putts. In practice, however, top professionals in tournaments only hole about 17% of 4-m putts. We conclude that the putting stroke of elite golfers has a relatively minor influence on direction consistency.