Elimination of lipaemic interference by high-speed centrifugation

IntroductionIn order to deliver high quality results, detection and elimination of possible analytical interferences, such as lipaemia, is crucial. The aim of this study is to evaluate the efficacy of high-speed centrifugation in eliminating lipaemic interference and to define own lipaemic index (LI) for the studied biochemical analytes.Materials and methodsEvaluated analytes were: albumin, alkaline phosphatase, alanine-aminotransferase (ALT), aspartate-aminotransferase (AST), calcium, creatinine, gamma-glutamyltransferase (GGT), glucose, phosphates, total proteins, urea and total bilirubin. Those analytes and LIs have been analysed in duplicate in the Roche Diagnostics-c8000 analyser in samples centrifuged at 3000 rpm/10 minutes in the SL16 (Thermo Scientific, Waltham, USA) centrifuge and according to an own high-speed centrifugation protocol (12,900 rpm/15 minutes) in the MicroCL17R (Thermo Scientific, Waltham, USA) centrifuge. Lipaemia has been measured in each sample. The efficiency of high-speed centrifugation is verified by the Wilcoxon test (P < 0.05). In cases where significant differences are observed, our own LI is calculated. For ALT and AST, it is verified by McNemar test (P < 0.05). For creatinine, both Wilcoxon and McNemar test were applied.ResultsThere were statistically significant differences in analyte concentration before and after high-speed centrifugation for: albumin, creatinine, GGT, glucose, phosphates, urea and total bilirrubin. Own LI is calculated. McNemar test shows statistically significant diferences in the proportion of delivered results before and after high-speed centrifugation in ALT, AST and creatinine.ConclusionsThis study confirms the efficacy of high-speed centrifugation protocol for all the considered analytes, excepting calcium, alkaline phosphatase and total proteins.     

The relationships among consumers’ ethical ideology,risk aversion and ethically-based distrust of online retailers and the moderating role of consumers’ need for personal interaction

Consumer distrust is only recently beginning to be perceived as an important e-commerce issue and, unlike online trust, the nature and role of distrust is much less established. This study examines the influence of two important consumer characteristics (ethical ideology and risk aversion) on consumer’s ethically-based distrust of online retailers. Also, the moderating role of consumer’s need for personal contact with sales staff is tested. Results from 409 online consumers confirm that both relativist-based ethical ideology and risk aversion are strongly and positively related to consumers’ distrust. Interestingly, our findings show that positive effects of relativism and risk aversion on consumer’s distrust are moderated by consumers’ need for personal interaction, which is more pronounced for those consumers with a high need for personal interaction with retail salespeople.     

Spreading the digital word: ISI’s Electronic Library Project

Describes the process behind – and the progress of – a unique project designed to explore many of the technical and economic issues pertaining to the implementation and use of an electronic library.     

Playing surface and UK professional rugby union injury risk

Artificial rugby union playing surface installation is increasing. This prospective cohort study aimed to examine the effect of playing surface on match injury types within 157 players of two UK professional rugby union clubs playing 209 matches (96 on artificial surfaces and 113 on grass) over three seasons. There was no difference in overall injury risk between the two playing surfaces with injury incidence on artificial 80.2 (CI 69.9–91.7) and on grass 81.9 per 1000 match-hours (CI 72.2–92.5), with an incidence rate ratio (RR) of 0.98 (CI 0.82–1.17). There was a higher rate of concussion (RR 0.52, CI 0.34 – 0.78) and chest injuries on grass (RR 0.26 CI 0.07, 0.95), and a higher rate of thigh haematoma (RR 2.25, CI 1.05–4.82) foot injuries (RR 4.12, CI 1.10, 15.40) and injury to players being tackled (RR 1.46, CI 1.00, 2.15) on artificial. Whilst there was no higher injury risk for matches played on artificial versus natural grass surfaces, the higher incidence of concussion and chest injury on grass, and the higher rate of foot injuries on artificial surfaces may be related to tackle and footwear-to-surface interface factors.     

Sex education in Spain: the relevance of teachers’ training and attitudes / Educación sexual en España: importancia de la formación y las actitudes del profesorado

Abstract

This research has two main aims: a) the study of Spanish teachers’ training and attitudes towards sex education, as well as the delivery of this subject in classrooms, while also exploring the existence of differences according to personal and professional characteristics; and b) to examine the association between teachers’ training and attitudes, and the extent to which these variables predict the delivery of sex education. A total of 3,695 Infant, Primary, Secondary and High school teachers (66.1% women, 33.9% men) throughout the country completed a voluntary online questionnaire that inquired about their training and attitudes towards sex education, their delivery or non-delivery of this subject in classrooms, and other personal and professional characteristics. Analyses indicate that participants show positive attitudes towards sex education, but that 43.3% are not trained in this issue, and 48.6% do not teach it at school. Some significant differences by sex, educational stage and type of school have been found. In addition, trained teachers show more positive attitudes to sex education. Finally, having more positive attitudes and, to a lesser extent, having prior training, positively predicts the delivery of sex education. These results highlight the importance of reviewing and improving teachers’ training on sexuality and sex education, with particular emphasis on attitudinal contents.     

Effective and inclusive schools? Attention to diversity in highly effective schools in the Autonomous Region of the Basque Country

This paper forms part of a research project that aims to characterise best practices in highly effective schools in the Autonomous Region of the Basque Country (ARBC). The aim is for the best practices identified to serve as points of reference when designing improvement plans to be implemented in all schools in the ARBC, with the advice and support of the education inspectorate. Highly effective schools are identified through the results of the Diagnostic Assessments carried out by the Basque Institute for Research and Evaluation in Education (ISEI-IVEI) of all primary and secondary schools in the ARBC. Multilevel statistical modelling and Hierarchical Linear Models were used to select 32 highly effective schools, with highly effective being taken to mean any school with higher-than-expected student results after adjusting for the effect of contextual factors. Eighty-three interviews were conducted with management teams, educational advisors and inspectors at each of the selected schools. This paper presents the results obtained in relation to how attention to diversity is organised. The practices identified included, among others, inclusive actions such as support teaching in ordinary classrooms, the splitting up of classroom groups, the formation of heterogeneous groups and the conceptualisation of the support provided as a shared teaching responsibility.     

Systematic Review: Flipped Classrooms in the Performance of Undergraduate Science Students

Journal of Science Education and Technology - The sciences are a complex and especially demanding area of study for undergraduate students, particularly in the earlier years, which are a critical...