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981.
Katja Uhlig Bj?rn Boysen Andreas Lankenau Magnus Jaeger Erik Wischerhoff Jean-Fran?ois Lutz André Laschewsky Claus Duschl 《Biomicrofluidics》2012,6(2)
Thermoresponsive polymer surface coatings are a promising tool for cell culture applications. They allow for a mild way of cell detachment that preserves the activity of membrane proteins—a prerequisite for reliable cell analysis. To enlarge the application range of these coatings to cells with different adhesion properties, we synthesized various novel poly(ethylene glycol)-based thermoresponsive polymers and describe how (i) their chemical structure and (ii) their surface density affect their efficiency. In order to quantify the influence of both factors, the time for cell spreading and rounding efficiency were observed. As a result, efficiency of cell rounding, which is closely correlated to cell detachment, is less affected by both factors than the time needed for cell spreading. This time can effectively be adjusted by the molecular architecture which includes the length of the polymer backbone and the side chains. Based on this work, recommendations are given for future optimization of functionality of thermoresponsive polymer coatings for cell culture applications. 相似文献
982.
Scafoglieri A Tresignie J Provyn S Marfell-Jones M Reilly T Bautmans I Clarys JP 《Journal of sports sciences》2012,30(8):777-785
The aim of the present study was to develop and cross-validate anthropometrical prediction equations for segmental lean tissue mass (SLM). One hundred and seventeen young healthy Caucasians (67 men and 50 women; mean age: 31.9 ± 10.0 years; Body Mass Index: 24.3 ± 3.2 kg · m(-2)) were included. Body mass (BM), stretch stature (SS), 14 circumferences (CC), 13 skinfolds (SF) and 4 bone breadths (BB) were used as anthropometric measurements. Segmental lean mass of both arms, trunk and both legs were measured by dual energy X-ray absorptiometry as the criterion method. Three prediction equations for SLM were developed as follows: arms = 40.394(BM) + 169.836(CCarm-tensed) + 399.162(CCwrist) - 85.414(SFtriceps) - 39.790(SFbiceps) - 7289.190, where Adj.R (2) = 0.97, P < 0.001, and standard error of estimate (SEE) = 355 g;trunk = 181.530(BM) + 155.037(SS) + 534.818(CCneck) + 175.638(CCchest) - 88.359(SFchest) - 147.232(SFsupraspinale) - 46522.165, where Adj.R(2) = 0.97, P < 0.001, and SEE = 1077g; and legs = 55.838(BM) + 88.356(SS) + 235.579(CCmid-thigh) + 278.595(CCcalf) + 288.984(CCankle) - 84.954(SFfront-thigh) - 53.009(SFmedial calf) - 28522.241, where Adj.R (2) = 0.96, P < 0.001, and SEE = 724 g. Cross-validation statistics showed no significant differences (P < 0.05) between observed and predicted SLM. Root mean squared errors were smallest for arms (362 g), followed by legs (820 g) and trunk (1477 g). These new prediction equations allow an accurate estimation of segmental lean mass in groups of young adults, but estimation errors of 8 to 14% can occur in certain individuals. 相似文献
983.
Science, technology and innovation have grown in importance over the last 50 years as we have moved towards a more knowledge-intensive society (the ‘knowledge society’). A number of new research fields have emerged in an effort to understand these developments and to offer advice to decision-makers in government, industry and elsewhere. This special issue focuses on studies of three relatively distinct though thematically related research fields (innovation studies, entrepreneurship studies, and science and technology studies). The first three articles use a particular methodology based on analysis of the references cited in the chapters to authoritative ‘handbooks’ to identify the core contributions in the three fields. A fourth article examines the relationship between the core literatures in three fields and how this has evolved over time. Other articles look at the evolution of innovation studies as reflected in highly cited papers, at the development of entrepreneurship as seen by a key ‘insider’, and at the creation of new centres in these fields and the difficulties they face. The last article in this special issue shows how interdisciplinary centres in innovation studies suffer from research assessment systems that are intrinsically biased against interdisciplinary research. This introduction presents a synthesis of the articles in this special issue, discusses similarities and differences between the three fields and their development over time, and considers challenges for policy and governance arising from the research presented here. 相似文献
984.
985.
986.
In this article Joris Vlieghe, Maarten Simons, and Jan Masschelein attempt to articulate a new way of dealing with the public character of education. Instead of discussing laughter as an instrument that one could use to facilitate established educational goals, the authors provide an extensive analysis of the phenomenon of laughter as a specific form of corporeal behavior. Their analysis demonstrates that when we laugh, we give an answer to a disorienting situation, but this answer is not the product of intentional agency. Instead, it consists in the uncontrollable spasmodic contraction of our diaphragm and other impersonal and automatic corporeal reactions. We are thus exposed to an ultimate loss of self‐control. The authors argue further that communal laughter—that is, when the lack of mastery over our own lives becomes a shared experience—results in what they call a “democracy of the flesh.” In this state, it is no longer possible to stick to well‐defined positions, nor is it tenable to defend any hierarchical ordering, including the strict hierarchy we typically find in the context of schooling and education. Common laughter makes equals out of us and grants the possibility of an unforeseen and unimaginable future. For precisely this reason, the authors conclude, communal laughter might be considered as an educational event itself. 相似文献
987.
Animated models explicating how a problem is solved and why a particular method is chosen are expected to be effective learning
tools for novices, especially when abstract cognitive processes or concepts are involved. Cognitive load theory was used to
investigate how learners could be stimulated to engage in genuine learning activities. It was hypothesized that illusion of
control would impede transfer performance compared to a condition without illusion of control. Moreover, we hypothesized that
learners who first studied an animated model and then solved the same problem would perform better on transfer than learners
who studied the same animated model twice or who first solved the problem and then studied the animated model. In a 2 × 3
factorial experiment (N = 90) with the factors illusion of control (yes vs. no) and instruction method (study–practice, practice–study, study–study) only the first hypothesis was confirmed. Implications for the design of animated
models are discussed. 相似文献
988.
989.
Anne-Marie Nu?ez Elizabeth Murakami Ramalho Kimberley K. Cuero 《Innovative Higher Education》2010,35(3):177-190
Three female tenure-track faculty members at a Hispanic-Serving Institution explored how their cultural backgrounds inform
their pedagogical approaches toward equity. They drew upon Mills’s (1959) and Collins’s (1993) frameworks to examine how their personal biographies, local social contexts, and broader systemic institutions affect their
teaching processes for diverse students. These teaching processes include limiting assumptions about students, encouraging
students to consider their own personal biographies in relation to the social world, welcoming students’ multiple modes of
expression, serving as role models, and challenging inequities in schooling. They conclude with recommendations for enhancing
inclusivity in student learning and faculty development. 相似文献
990.