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91.
Alex Livy Sayhean Lye Chahil K. Jagdish Nurul Hanis Velapasamy Sharmila Lian Wee Ler Bagali Pramod 《Indian journal of clinical biochemistry : IJCB》2012,27(1):28-33
Buccal cell usage has been shown by many to be a cost effective and safe method to isolate DNA for various biological experiments
especially large epidemiological studies (Garcia-Closas et al. Cancer Epidemiol Biomarkers Prev 10:687–696, 2001). Non-invasive DNA collection methods are preferred over phlebotomy in order to increase study participation and compliance
in research centers and for sick patients in hospital settings. There have been conflicting reports about the methodology
and results obtained from using buccal DNA. It is not very clear if phlebotomy can be confidently replaced by buccal cell
DNA. It is often left for the user to take an intelligent decision. To address this issue, we compared the performance of
buccal and blood DNA from same subjects in a genotyping experiment and this paper reports the results. Cotton swab derived
buccal cells were scraped from the inner side of cheeks from 16 subjects, and blood was also drawn from the same 16 subjects
participating in a genotypic association study of a lipid disease. The DNA quality was assessed by resolving on agarose gels,
checking purity (A260/A280) and finally by microarray hybridization. This study showed that DNA degradation affects the total
yield and performance of the buccal DNA when compared to the blood DNA in microarray based genotyping. Genotyping results
can be seriously compromised if care is not taken to check the quality and yields of such specimens. 相似文献
92.
Daniel F. B. Haeufle Jay Worobets Ian Wright Jenny Haeufle Darren Stefanyshyn 《Sports Engineering》2012,15(4):215-220
A common belief in the golf community is that a lighter shaft allows the golfer to swing the club faster. From a mechanical point of view, reducing the mass of the shaft would result in a faster swing. However, a golfer is not a purely mechanical system, and so it is simplistic to assume that identical loads will be applied when swinging different clubs. Therefore, the purpose of this study was to test the hypothesis that golfers behave similar to a mechanical model when swinging clubs of varying mass. A torque driven model estimated the effects caused by the addition of 22?g to the shaft. Twelve golfers hit balls with a standard driver as well as a driver fitted with the same 22?g increase in mass. Club kinematics were collected with a high-speed motion capture system. The model predicted a 1.7?% lower club head speed for the club with additional mass. One subject showed a similar reduction (1.4?%), but one subject showed an increase in club head speed by 3.0?%. Ten subjects did not show any significant differences. These results suggest that golfers do not respond to changes in club mass in a mechanically predictable way. 相似文献
93.
Governing education through data: Scotland,England and the European education policy space 总被引:1,自引:0,他引:1
This paper draws on interview data from national policy makers in England, Scotland and the European Commission to illustrate differences in the referencing of ‘Europe’ in education policy‐making in England and Scotland in order to highlight the emergent complexity of post‐devolution policy‐making in education through a focus on relations and interactions with Europe, as expressed in the negotiation and development of performance data systems. We suggest that policy‐makers in England reference global influences, rather than Europe, while policy‐makers in Scotland reference Europe in order to project a new positioning of Scotland in closer alignment with Europe. Europeanisation in education thus produces differing policy responses from closely aligned, indeed, in the case of England and Scotland, contiguous policy spaces. Thus the paper seeks to contribute to the literature on ‘travelling’ education policy and its ‘local’ mediation and to connect the development of devolution and the changing policy space of education in Europe. 相似文献
94.
As definitions of literacy become reconceptualised, so too do constructions of the primary teacher's identity. This study analyses the discourses that emerged from interviews with a group of Australian primary teachers who talked about their reactions to teaching media. Teachers who embraced this area of learning had a more futures-oriented view of their identity. However, there were still teachers who retained a more traditionalist perception of their role, including some who viewed teaching media as an unnecessary burden. These contrasting perspectives suggest that a crisis in identity will remain unless the boundaries that separate conventional literacies from new literacies can be challenged. 相似文献
95.
The purpose of this study was to extend understanding of how athletes and coaches in a women’s cycling talent development and selection programme negotiate and normalise athlete abuse in the media. A thematic analysis of six online cycling magazine articles and their representations of the Australian women’s elite cycling development camp was analysed to explore athletic abuse and the (re)production of coaching practices using Bourdieusian theory. The findings revealed a link between the expression of coaching practice and the maltreatment of athletes. Analysis of these articles also revealed that athletes were complicit in the normalisation of coaching practices through the misrecognition of social power embedded in the coaching intervention. The representations by athletes within the articles contributed narratives related to the reproduction and proliferation of abusive coaching practices. This study extends understanding of how taken for granted and power laden aspects of coaching practices can be presented in the media and highlights the implications for coaches, athletes and the general public that consume online cycling media content. 相似文献
96.
This paper explores three female swimmers’ relationships with their male coaches and the body practices they were exposed to within Australian swimming. Particular attention is given to how the relationships and practices might relate to gender. Additionally, the article examines how (if at all) the conduct contributed to the social construction of an accepted female swimmer body. Through narrative accounts, the three adolescent female athletes articulate hierarchical male coach–female athlete relationships and specific body encounters they were exposed to and/or engaged with. Their experiences reveal how a sexually maturing body (growing breasts, female body shape and menstruating) was deemed unsuitable for performance and the swimmers were thus encouraged to transform their bodies and behaviours towards that of the boys. Using a feminist Foucauldian perspective, these accounts points to how the three swimmers came to regulate their diet, training and appearance in order to fulfil expectations. This self-regulation is problematic in two ways: first, no scientific evidence shows that a boy like physique is essential for best performance. Second, the stress from being pressured to achieve a particular body, as well as the shame that resulted from being unable to achieve the idealised physique, eventually caused the swimmers to develop an unhealthy relationship with their developing bodies. We highlight how those immersed in sporting contexts should recognise the serious implications of gender practices and power relations underpinning the male coach–female athlete dynamic in competitive sport. 相似文献
97.
Sharon Connell John Fien Jenny Lee Helen Sykes David Yencken 《Environmental Education Research》1999,5(1):95-113
This article presents a range of findbigs from a qualitative study of the environmental attitudes of young people across their final 2 years of secondary school in the two Australian cities of Melbourne and Brisbane. Focus groups comprising the same 16‐ to 17‐year‐old students in 12 schools were interviewed twice, 12 months apart. Several minor differences were found in the attitudes of students between the two cities, but these pale alongside the common, indeed, overwhelming feelings of environmental concern mixed with frustration, cynicism and action paralysis that were reported. The ambivalence towards the environment that results, together with the individualistic frameworks for explaining environmental issues that were displayed, point to areas for renewed curriculum attention in secondary schools and directions for future research. 相似文献
98.
Dart Barry Burnett Paul Boulton-Lewis Gillian Campbell Jenny Smith David McCrindle Andrea 《Learning Environments Research》1999,2(2):137-156
Four hundred and eighty-four students from two metropolitan secondary schools completed the Learning Process Questionnaire,
the Individualised Classroom Environment Questionnaire and the Learner Self Concept scale. Relationships between perceptions
of the classroom learning environment, approaches to learning and self concept as a learner were investigated. Gender and
level of schooling (junior high versus senior high) differences were examined. Results showed Deep Approaches to learning
were significantly related to classroom learning environments which were perceived to be highly personalised and to be encouraging
active participation in the learning process and the use of investigative skills in learning activities. High learner self
concept was positively associated with Deep Approaches to learning and with classrooms perceived as high in Personalisation.
It was negatively associated with Surface Approaches to learning. Differences in perceptions of learning environments and
approaches to learning in relation to gender and level of schooling were small. The implications of these findings are discussed
and strategies for facilitating Deep Approaches to learning are referred to.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
99.
The aim of this study was to examine the effect of movement and stretching imagery on increases in flexibility. Thirty volunteers took part in a 4 week flexibility training programme. They were randomly assigned to one of three groups: (1) movement imagery, where participants imagined moving the limb they were stretching; (2) stretching imagery, where participants imagined the physiological processes involved in stretching the muscle; and (3) control, where participants did not engage in mental imagery. Active and passive range of motion around the hip was assessed before and after the programme. Participants provided specific ratings of vividness and comfort throughout the programme. Results showed significant increases in flexibility over time, but no differences between the three groups. A significant relationship was found, however, between improved flexibility and vividness ratings in the movement imagery group. Furthermore, both imagery groups scored significantly higher than the control group on levels of comfort, with the movement imagery group also scoring significantly higher than the stretching imagery group. We conclude that the imagery had stronger psychological than physiological effects, but that there is potential for enhancing physiological effects by maximizing imagery vividness, particularly for movement imagery. 相似文献
100.
This paper argues for a historical analysis of the relationship between teachers and the state. The historical analysis demonstrates the ways in which strategies of teacher management shifted according to circumstances. In the first period, the 1920s, restrictions on teacher autonomy were removed, and ‘indirect rule’ used as a means of moving teachers away from the labour movement. In the second period, after the 1944 Act, a restricted ‘professional’ autonomy was encouraged. In both periods equality of status for teachers as ‘partners’ in the educational enterprise was offered in return for teachers’ acceptance of a limited or licensed professionalism. Current developments in the management of the teaching force suggest that the abandonment of indirect strategies of management may result in a return by teachers to less restricted ‘professional’ behaviour. 相似文献