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211.
212.
Eriksen J Thilsted AH Marie R Lüscher CJ Nielsen LB Svendsen WE Szabo P Kristensen A 《Biomicrofluidics》2011,5(3):31101-311014
A method of in situ chromosome immobilisation and DNA extraction in a microfluidic polymer chip was presented. Light-induced local heating was used to induce poly(N-isopropylacrylamide) phase transition in order to create a hydrogel and embed a single chromosome such that it was immobilised. This was achieved with the use of a near-infrared laser focused on an absorption layer integrated in the polymer chip in close proximity to the microchannel. It was possible to proceed to DNA extraction while holding on the chromosome at an arbitrary location by introducing protease K into the microchannel. 相似文献
213.
Morten Bredsgaard Randers Jesper L. Andersen Jesper Petersen Emil Sundstrup Markus D. Jakobsen Jens Bangsbo 《Journal of sports sciences》2014,32(13):1300-1308
AbstractThe aim was to investigate performance variables and indicators of cardiovascular health profile in elderly soccer players (SP, n = 11) compared to endurance-trained (ET, n = 8), strength-trained (ST, n = 7) and untrained (UT, n = 7) age-matched men. The 33 men aged 65–85 years underwent a testing protocol including measurements of cycle performance, maximal oxygen uptake (VO2max) and body composition, and muscle fibre types and capillarisation were determined from m. vastus lateralis biopsy. In SP, time to exhaustion was longer (16.3 ± 2.0 min; P < 0.01) than in UT (+48%) and ST (+41%), but similar to ET (+1%). Fat percentage was lower (P < 0.05) in SP (–6.5% points) than UT but not ET and ST. Heart rate reserve was higher (P < 0.05) in SP (104 ± 16 bpm) than UT (+21 bpm) and ST (+24 bpm), but similar to ET (+2 bpm), whereas VO2max was not significantly different in SP (30.2 ± 4.9 ml O2 · min?1 · kg?1) compared to UT (+14%) and ST (+9%), but lower (P < 0.05) than ET (?22%). The number of capillaries per fibre was higher (P < 0.05) in SP than UT (53%) and ST (42%) but similar to ET. SP had less type IIx fibres than UT (?12% points). In conclusion, the exercise performance and cardiovascular health profile are markedly better for lifelong trained SP than for age-matched UT controls. Incremental exercise capacity and muscle aerobic capacity of SP are also superior to lifelong ST athletes and comparable to endurance athletes. 相似文献
214.
Martin Pinquart Jens P. Pfeiffer 《International Journal of Disability, Development & Education》2012,59(2):145-155
The present study compared psychological adjustment of 158 adolescents with vision impairment and 158 sighted adolescents with a matched-pair design using the Strengths and Difficulties Questionnaire. Adolescent self-reports and teacher reports on emotional problems, peer problems, and total difficulties showed higher scores in students with vision impairment than in sighted students. However, the size of between-group differences was small, and adolescents who are blind and their peers with low vision showed similar levels of psychological adjustment. In addition, older students reported more emotional problems than younger students. Furthermore, differences between self-reported emotional problems of students with and without vision impairment were stronger in older participants. In both groups, girls showed more emotional problems and less conduct problems than boys. We conclude that most students with vision impairment are well adjusted but a minority may benefit from psychological interventions. 相似文献
215.
Jens P. Pfeiffer Martin Pinquart Hannes Münchow 《European Journal of Psychology of Education - EJPE》2012,27(3):389-402
The present study analyzed whether the perceived attainment of developmental goals differs by school type and between adolescents with visual impairments and sighted peers. We created a matched-pair design with 98 German students from the middle school track and 98 from the highest school track; half of the members of each group were visually impaired. Students from the middle school track reported a higher attainment of identity development, career choice, and gender role awareness than students from the highest school track. Furthermore, students with visual impairment were less progressed with peer-group integration and forming intimate relationships than sighted students, but they reported higher levels of attainment of skills that are important for their future profession. However, we did not find an overall interaction effect between school type and vision status on the perceived attainment of developmental goals. The observed school type differences can be explained by the fact that students from the middle school track face the transition from school to work earlier. Implications for practice are discussed. 相似文献
216.
Jens‐Christian Smeby 《Teaching in Higher Education》2013,18(1):5-20
Abstract Even though the interaction between teaching and research at universities is a well‐studied topic, it is difficult on the basis of available studies to draw any definite conclusions concerning the character of this relationship. This study, which is based on survey data, as well as interviews, shows that university faculty believe that their research influences their teaching and that their teaching activities have a positive effect on their teaching. They find, however, that research is more important for teaching than vice versa. The characteristics of the interaction vary between teaching on various levels and between disciplines. In general, the interaction is stronger at a graduate than at an undergraduate level. At an undergraduate level the relationship is stronger in the humanities and the social sciences than in other fields of learning, while there are no such differences at a graduate level. The findings are discussed on the basis of differences between disciplines and types of teaching 相似文献
217.
218.
Astrid Heidemann Lassen Suna Løwe Nielsen 《Research in Science & Technological Education》2013,31(3):275-290
Background: Globalization, technological advancement, environmental problems, etc. challenge organizations not just to consider cost-effectiveness, but also to develop new ideas in order to build competitive advantages. Hence, methods to deliberately enhance creativity and facilitate its processes of development must also play a central role in engineering education. However, so far the engineering education literature provides little attention to the important discussion of how to develop knowledge intensive ideas based on creativity methods and concepts. Purpose: The purpose of this article is to investigate how to design creative camps from which knowledge intensive ideas can unfold. Design/method/sample: A framework on integration of creativity and knowledge intensity is first developed, and then tested through the planning, execution and evaluation of a specialized creativity camp with focus on supply chain management. Detailed documentation of the learning processes of the participating 49 engineering and business students is developed through repeated interviews during the process as well as a survey. Results: The research illustrates the process of development of ideas, and how the participants through interdisciplinary collaboration, cognitive flexibility and joint ownership develop highly innovative and knowledge-intensive ideas, with direct relevance for the four companies whose problems they address. Conclusions: The article demonstrates how the creativity camp methodology holds the potential of combining advanced academic knowledge and creativity, to produce knowledge intensive ideas, when the design is based on ideas of experiential learning as well as creativity principles. This makes the method a highly relevant learning approach for engineering students in the search for skills to both develop and implement innovative ideas. 相似文献
219.
Jan Alexis Nielsen 《International Journal of Science Education》2013,35(5):723-744
This paper explores how students invoked different conceptions of ‘nature’ in eight socio-scientific group discussions about human gene therapy. The paper illustrates and discusses how the students articulated nature and to what extent they elicited science factual content in the process. While the students in this study invoked nature at key places in a variety of dialectical contexts in the discussions, these invocations were often uncritical appeals and rarely involved science factual content. Even when an argument from nature was challenged, the author of that argument would often shift the sense of nature rather than elaborate upon the argumentation. It is argued that if students were properly introduced to the evaluative character of the term ‘nature’ it would not just be conducive to the quality of their argumentation, but also invite them to foreground science factual content at key places in their discussion. 相似文献
220.
Jens Rasmussen 《课程研究杂志》2013,45(5):569-582
Scaffolding as a metaphor for support of learning processes places focus on the relationship between the psychic and social aspects of those processes. This paper examines the conceptualization of this relationship in activity theory and in operational constructivism. The importance of a distinction between psychic processes, language and communication is especially emphasized and discussed in relation to understandings of empathy, inter-subjectivity, and distributed cognition. 相似文献