Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo

BackgroundConsidering the potential cumulative effects of repetitive head impact (HI) exposure, we need sensitive biomarkers to track short- and long-term effects. Circulating small extracellular vesicles (sEVs) (<200 nm) traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease. The purpose of this study was to identify the microRNA (miRNA) profile in circulating sEVs derived from human plasma following repetitive HI exposure.MethodsHealthy adult (aged 18–35 years) soccer players were randomly assigned to one of 3 groups: the HI group performed 10 standing headers, the leg impact group performed 10 soccer ball trapping maneuvers over 10 min, and the control group did not participate in any soccer drills. Plasma was collected before testing and 24 h afterward, and sEVs were isolated and characterized via nanoparticle tracking analysis. Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs, and candidate microRNAs were analyzed via quantitative polymerase chain reaction. In silico target prediction was performed using TargetScan (Version 7.0; targetscan.org) and miRWalk (http://mirwalk.umm.uni-heidelberg.de/) programs, and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney (HEK) 293T/17 cells.ResultsPlasma sEV concentration and size were not affected across time and group following repetitive HI exposure. After 24 h, the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p, miR-423-5p, and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p, miR-144-5p, miR-221-5p, and miR-22-3p. Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels. To our knowledge, miR-7844-5p is a previously unknown miRNA. We identified 8 miR-7844-5p mRNA targets: protein phosphatase 1 regulatory inhibitor subunit 1B (PPP1R1B), LIM and senescent cell antigen-like domains 1 (LIMS1), autophagy-related 12 (ATG12), microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), integrin subunit alpha-1 (ITGA1), mitogen-activated protein kinase 1 (MAPK1), glycogen synthase kinase 3β (GSK3β), and mitogen-activated protein kinase 8 (MAPK8).ConclusionCollectively, these data indicate repetitive HI exposure alters plasma sEV miRNA content, but not sEV size or number. Furthermore, for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis, autophagy regulation, mood disorders, and neurodegenerative disease.     

Health education for Somali Bantu refugees via home visits

Background: Somali Bantu refugees, with unique health information needs, created challenges for health and social service providers. Objectives: A service innovation was developed (i) to raise awareness, especially among local health and social service providers, about the Bantu refugees’ presence in the community, their culture, and their information needs and (ii) to deliver needed health information, emphasizing child health, to the Bantu mothers in their homes. Methods: The project consisted of: (i) a community conference targeting local health and social service providers, describing the refugees’ presence in the community, their culture, and information needs. (ii) Focus groups conducted with members of the Bantu population elicited additional information needs. (iii) Curriculum was developed based on identified needs, and (iv) the curriculum was delivered to the refugees in their homes. A clinical informationist and MP3 technology enhanced the project. Findings: Conference attendees’ evaluation responses indicated improved understanding of Bantu culture. Focus groups’ identification of health information needs provided a framework for the health education curriculum. A project website made educational materials available to other healthcare providers. Conclusions: The project raised awareness of the Bantus’ presence, culture, and information needs. Identification of other unmet needs demonstrated that additional support for refugees is required.     

EUROPEAN PUBLIC PERCEPTIONS OF SCIENCE

In this paper we explore the relationships between public interestin, knowledge of, and attitudes towards science and technologyand the level of industrialization in 12 countries of the EuropeanCommunity. Using the 1989 Eurobarometer survey no 31 (N=11,678),we constructed four scales measuring interest, knowledge, generalattitudes to science, and support for EC funded science foreach of the 12 EC countries. We found that overall factual scientificknowledge correlates moderately positively with interest andattitudes to science. For example the Netherlands and GreatBritain have relatively high levels of knowledge, interest,and support for science in general. Spain and Greece have relativelylow levels of knowledge, interest, and support for science ingeneral. However, the simple correlation hides more complexrelationships. First, the variance within and across countriesof knowledge, attitudes and interest increases with nationallevels of knowledge. This indicates greater degrees of polarizationof both support and interest. Second, the data indicates a post-industrialismeffect: knowledge, interest in, and attitudes to science showa curvilinear relationship with levels of industrialization.The decline of interest in science and the less positive attitudesin highly developed countries requires further exploration.Third, we found, that the consistency of the knowledge and theattitude measures declines as the national level of knowledgeincreases. We suggest that this reflects a knowledge-ignoranceparadox and knowledge specialization among informed populations.Support for EC level science follows a different pattern. Wefound that France, Italy, and Spain show high levels of supportfor EC level science. This is not the case in Great Britainand Germany. The pattern suggests that attitudes towards ECscience are formed on the basis of national level scientificstrength: if the national science base is strong, EC scienceinitiatives find less support and vice versa. These findingsrepresent a first step towards a comparative assessment of publicunderstanding of science in EC countries.     

Export restrictions,tax incentives and the national artistic patrimony

This paper analyses the main forms of government intervention used in the UK to protect the national artistic patrimony. It examines the two most common policy measures used in the art market ‐ export regulation and tax incentives ‐ and reports their use over an eight‐year period from 1990 to 1998. It also reviews the UK's collective obligations to patrimony internationally and specifically as a member of the European Union. This is the first time that these related topics have been considered as a whole.     

A Detection Scheme for Dependent Noise Processes

In this paper the problem of memoryless discrete time detection of a constant signal in additive φ-mixing noise is considered. The fidelity criterion is taken to be the asymptotic relative efficiency. The optimal detector nonlinearity is approximated by a polynomial, and the statistical knowledge required for the design of the detector is the family of joint moments of the noise, resulting in a very simple design procedure for the detector. It is shown that the performance of this detector can be made arbitrarily close to that of the optimal memoryless detector for a large class of noises.