Isolation and enrichment of low abundant particles with insulator-based dielectrophoresis

Isolation and enrichment of low-abundant particles are essential steps in many bio-analytical and clinical applications. In this work, the capability of an insulator-based dielectrophoresis (iDEP) device for the detection and stable capture of low abundant polystyrene particles and yeast cells was evaluated. Binary and tertiary mixtures of particles and cells were tested, where the low-abundant particles had concentration ratios on the order of 1:10 000 000 compared to the other particles present in the mixture. The results demonstrated successful and stable capture and enrichment of rare particles and cells (trapping efficiencies over 99%), where particles remained trapped in a stable manner for up to 4 min. A device with four reservoirs was employed for the separation and enrichment of rare particles, where the particles of interest were first selectively concentrated and then effectively directed to a side port for future collection and analysis. The present study demonstrates that simple iDEP devices have appropriate screening capacity and can be used for handling samples containing rare particles; achieving both enrichment and isolation of low-abundant particles and cells.     

Family patterns and child abuse in a nationally representative American sample

Data on 1, 146 families with a child age 3 through 17 at home are presented. Child abuse information was obtained for a randomly selected child in each family. Child abuse was defined as an attack by a parent involving punching, kicking, biting, hitting with an object, “beating up,” or using a knife or gun. Over 14 out of every 100 American children 3–17 are subjected to abusive violence each year. These figures are at least 26 times greater than those of the National Center For Child Abuse and Neglect. Even so, for reasons outlined in the paper, they are underestimates. Families in which child abuse occurred are compared with other families. The results suggest that child abuse is brought about by the very nature of the society and its family system. This has profound implications for the prevention and treatment of child abuse. Although psychotherapy may be appropriate in some cases, a more fundamental approach lies in such things as a more equal sharing of the burdens of child care, replacement of physical punishment with non-violent methods of child care and training, eliminating the stresses and insecurity which now characterize our economic system, and strengthening the ties of individual families to the extended family and the community.     

Universal metadata standard

Consciousness is based on the association of notions or a neural network. Similarly, the creation of the next-generation Internet (semantic web) is impossible without attributes that allow the semantic association of documents and their integration into an information context. To achieve these goals, the Universal Metadata Standard (UMS) may serve as a basis for documentography and is functionally required for interpretation of documents by automatic operating systems.     

Formation of the scientific concepts about the documental forms of information. The legal and source-study concepts of a document

The active use of documental information in various fields of activity has resulted in the formation of four main concepts of a document: the legal, source-study, gnoseological, and management concepts. The history of the origin and development of the legal and source-study concepts is considered. The significant influence that is exerted by the positivistic philosophy on the formation of these concepts is noted.     

Bookselling—book buying: The European perspective of the online market

The book market in European countries is different than the U.S. market for various political, geographical, and cultural reasons. The authors outline these differences and provide background on the move to electronic commerce and future. The article includes many electronic and print sources of and bibliographic tools for locating books in the European market.     

Analysis of publications of the pilot issue of the data collection of the All-Russia Institute of Scientific and Technical Information of the Russian Academy of Sciences “Industry of Nanosystems and Nanomaterials”

The pilot issue of the data collection of the All-Russian Institute of Scientific and Technical Information of the Russian Academy of Sciences “Industry of Nanosystems and Nanomaterials” gives an approximate view of the state of this field of science at the beginning of 2007. The topics of articles in the pilot issue are analyzed. The directions of development of nanotechnologies are shown. The basic types of nanomaterials, the methods of their production, and their properties and applications are given.