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71.
We have previously reported that microthrottle pumps (MTPs) display the capacity to pump solid phase suspensions such as polystyrene beads which prove challenging to most microfluidic pumps. In this paper we report employing a linear microthrottle pump (LMTP) to pump whole, undiluted, anticoagulated, human venous blood at 200 μl min(-1) with minimal erythrocyte lysis and no observed pump blockage. LMTPs are particularly well suited to particle suspension transport by virtue of their relatively unimpeded internal flow-path. Micropumping of whole blood represents a rigorous real-world test of cell suspension transport given blood's high cell content by volume and erythrocytes' relative fragility. A modification of the standard Drabkin method and its validation to spectrophotometrically quantify low levels of erythrocyte lysis by hemoglobin release is also reported. Erythrocyte lysis rates resulting from transport via LMTP are determined to be below one cell in 500 at a pumping rate of 102 μl min(-1). 相似文献
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A major concern of the governments of industrialised nations is the basis for the allocation of public resources to the private sector of industry. One particular area of debate is the role governments should play in the support and direction of high technology. This paper examines the contribution of the British government to the establishment of a computer industry and, in particular, the attempts to stimulate the industry during the period 1959 to 1962 by supporting the development and production of a ‘supercomputer’.The history of the negotiations between the three major participants, the National Research and Development Corporation, Ferranti Ltd., and Manchester University, has been reconstructed in considerable detail in order to provide the basis for an analysis of the interaction of technical, financial, organisational and political constraints in the attempts to establish the project'.The failure of NRDC to achieve its broad aims of promoting the future health of the computer industry is traced to three major problems — their failure to formulate clear objectives, their concern to maintain technical control, and inadequate policies for risk-sharing. These were exacerbated by the technical uncertainty associated with novel technology, an inadequate market analysis by Ferranti, and de facto technical direction by the university department.On this basis broad conclusions are drawn on the conditions necessary for a satisfactory return on public investment in high technology. 相似文献
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Pruyn EC Watsford ML Murphy AJ Pine MJ Spurrs RW Cameron ML Johnston RJ 《Journal of sports sciences》2012,30(1):71-78
Leg stiffness is a modifiable mechanical property that may be related to soft tissue injury risk. The purpose of this study was to examine mean leg stiffness and bilateral differences in leg stiffness across an entire professional Australian Football League (AFL) season, and determine whether this parameter was related to the incidence of lower body soft tissue injury. The stiffness of the left and right legs of 39 professional AFL players (age 24.4 ± 4.4 years, body mass 87.4 ± 8.1 kg, stature 1.87 ± 0.07 m) was measured using a unilateral hopping test at least once per month throughout the season. Injury data were obtained directly from the head medical officer at the football club. Mean leg stiffness and bilateral differences in leg stiffness were compared between the injured and non-injured players. There was no difference between the season mean leg stiffness values for the injured (219.3 ± 16.1 N x m(-1) x kg(-1)) and non-injured (217.4 ± 14.9 N x m(-1) x kg(-1); P = 0.721) groups. The injured group (7.5 ± 3.0%) recorded a significantly higher season mean bilateral difference in leg stiffness than the non-injured group (5.5 ± 1.3%; P = 0.05). A relatively high bilateral difference in leg stiffness appears to be related to the incidence of soft tissue injury in Australian football players. This information is of particular importance to medical and conditioning staff across a variety of sports. 相似文献
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Brenda Johnston 《Educational Action Research》2013,21(3):365-388
This article aims to explore one way of making connections between a teaching problem, a research process and a theory arrived at through action research. The article suggests this as a fruitful approach to pedagogic research in general. It focuses on a particular action research project on developing critical academic writing in an international context, illustrating how a particular teaching problem led to an action research investigation which resulted in a principled, theoretical framework to describe and guide the development of critical academic writing. Arriving at the theoretical framework involved the iterative interaction of theory with practice and required the integration of multiple theoretical perspectives. The theoretical framework was based on general principles or praxiology. There are few studies of this type on critical academic writing, or indeed any pedagogic issue, which seek to: (1) provide a framework for teaching; (2) operationalise this framework; (3) rigorously research its impact in in-depth case studies; and then (4) revise the framework in the light of reflection and analysis. 相似文献
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Lynne Wiltse Ingrid Johnston Kylie Yang 《Changing English: An International Journal of English Teaching》2014,21(3):264-277
In this paper we highlight findings from a teacher inquiry group study designed to explore possibilities for teaching contemporary Canadian literature to promote issues of social justice in secondary classrooms. Drawing on Boler and Zembylas’s notion of a ‘pedagogy of discomfort’, our paper will focus on the experiences of two teachers in the group who, through the selection and teaching of two Aboriginal Canadian texts, moved away from well-established pedagogical practices. We explore the role of the inquiry group in supporting teachers in their attempts to problematize unquestioned assumptions and address the absences in their curricular practices and examine the potential of using Canadian literature to enhance students’ understanding of historical marginalizations and structural inequalities. In conclusion, we discuss the implications of our research for pre-service and in-service educators who face the challenges of teaching in increasingly diverse schools. 相似文献
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