新疆锡伯族成年人城乡男女身体机能与全国指标比较分析

通过抽样调查新疆1500名锡伯族成年城乡男、女身体机能4项指标的测试、分析，揭示新疆锡伯族成年人的身体机能的变化规律、发展趋势，城乡与全国指标差异及其原因等，为研究锡伯族成年人机能变化规律和制定各项体制评定标准提供科学数据。     

新疆塔吉克族成年人城乡男、女身体机能与全国指标比较分析

通过抽样调查、测试和分析新疆帕米尔高原1500名塔吉克族城乡男、女成年人身体机能的4项指标,揭示了新疆塔吉克族成年人身体机能的变化规律、发展趋势,城乡及与全国指标差异及其原因等,为研究塔吉克族成年人机能变化规律和制定各项体质评定标准提供科学数据。结果显示,塔吉克族成年人身体机能低于全国平均水平。     

福建春季雷暴天气分析及灾害预测

2013年3月主要有2次冷空气过程,分别出现在19～20、23～24日.前期过程为西风槽东移,风暴结构特征为多超级单体的典型雷雨大风回波.200 hPa高空急流辐散强度和中低层急流强,垂直风切变大,地面倒槽更深,而后大范围显著升温,所以强天气现象更明显.后期过程为南支槽加深东移,过程以多单体或超级单体的断线型回波为主,冰雹强回波中心达到65 dbz,质心高且随高度的上升呈前倾类纺锤状.500 hPa降温幅度更大,冷锋移速更快,湿层更厚,所以短时强降水区域和强度更大.     

信息化技术与教学过程无缝融合的研究

为了实现信息化技术与教学过程的无缝融合,旨在分析目前信息化教学过程存在的问题并寻找引发这些问题的原因,进而探讨将信息化真正有效地融入教学过程的对策。采用归纳总结的方法,对目前信息化教学过程存在的问题和信息化教学过程有效性进行较为深入地研究。研究结果认为目前信息化教学过程存在人灌变电灌现象、过分强调学生的主体作用、落后的教学评价原则等问题,并从信息化教学理念、信息化教学目标、信息化教学情境、信息化教学评价等方面针对性地探讨实现有效性信息化教学的对策。研究结论认为,信息化技术与教学过程无缝融合对提高教育教学质量和培养高素质、创新型人才具有极其重要的现实意义,因此,寻求有效的信息化教学方法对实现信息化技术与教学过程的无缝融合凸显得尤为重要。     

Characterizations of kinetic power and propulsion of the nematode Caenorhabditis elegans based on a micro-particle image velocimetry system

Quantifying the motility of micro-organisms is beneficial in understanding their biomechanical properties. This paper presents a simple image-based algorithm to derive the kinetic power and propulsive force of the nematode Caenorhabditis elegans. To avoid unnecessary disturbance, each worm was confined in an aqueous droplet of 0.5 μl. The droplet was sandwiched between two glass slides and sealed with mineral oil to prevent evaporation. For motion visualization, 3-μm fluorescent particles were dispersed in the droplet. Since the droplet formed an isolated environment, the fluid drag and energy loss due to wall frictions were associated with the worm''s kinetic power and propulsion. A microparticle image velocimetry system was used to acquire consecutive particle images for fluid analysis. The short-time interval (Δt < 20 ms) between images enabled quasi real-time measurements. A numerical simulation of the flow in a straight channel showed that the relative error of this algorithm was significantly mitigated as the image was divided into small interrogation windows. The time-averaged power and propulsive force of a N2 adult worm over three swimming cycles were estimated to be 5.2 ± 3.1 pW and 1.0 ± 0.8 nN, respectively. In addition, a mutant, KG532 [kin-2(ce179) X], and a wild-type (N2) worm in a viscous medium were investigated. Both cases showed an increase in the kinetic power as compared with the N2 worm in the nematode growth medium due to the hyperactive nature of the kin-2 mutant and the high viscosity medium used. Overall, the technique deals with less sophisticated calculations and is automation possible.     

Microfluidic device capable of medium recirculation for non-adherent cell culture

We present a microfluidic device designed for maintenance and culture of non-adherent mammalian cells, which enables both recirculation and refreshing of medium, as well as easy harvesting of cells from the device. We demonstrate fabrication of a novel microfluidic device utilizing Braille perfusion for peristaltic fluid flow to enable switching between recirculation and refresh flow modes. Utilizing fluid flow simulations and the human promyelocytic leukemia cell line, HL-60, non-adherent cells, we demonstrate the utility of this RECIR-REFRESH device. With computer simulations, we profiled fluid flow and concentration gradients of autocrine factors and found that the geometry of the cell culture well plays a key role in cell entrapping and retaining autocrine and soluble factors. We subjected HL-60 cells, in the device, to a treatment regimen of 1.25% dimethylsulfoxide, every other day, to provoke differentiation and measured subsequent expression of CD11b on day 2 and day 4 and tumor necrosis factor-alpha (TNF-α) on day 4. Our findings display perfusion sensitive CD11b expression, but not TNF-α build-up, by day 4 of culture, with a 1:1 ratio of recirculation to refresh flow yielding the greatest increase in CD11b levels. RECIR-REFRESH facilitates programmable levels of cell differentiation in a HL-60 non-adherent cell population and can be expanded to other types of non-adherent cells such as hematopoietic stem cells.     

Development of flow through dielectrophoresis microfluidic chips for biofuel production: Sorting and detection of microalgae with different lipid contents

In this study, a continuous flow dielectrophoresis (DEP) microfluidic chip was fabricated and utilized to sort out the microalgae (C. vulgaris) with different lipid contents. The proposed separation scheme is to allow that the microalgae with different lipid contents experience different negative or no DEP force at the separation electrode pair under the pressure-driven flow. The microalgae that experience stronger negative DEP will be directed to the side channel while those experience less negative or no DEP force will pass through the separation electrode pair to remain in the main channel. It was found that the higher the lipid content inside the microalgae, the higher the crossover frequency. Separation of the microalgae with 13% and 21% lipid contents, and 24% and 30%–35% lipid contents was achieved at the operating frequency 7 MHz, and 10 MHz, respectively. Moreover, separation can be further verified by measurement of the fluorescence intensity of the neutral lipid inside the sorted algal cells.     

网络论坛质量评价的影响因素研究

网络论坛是重要的网络信息平台,本文从网站整体层次、版块层次和主题层次对影响网络论坛质量的因素进行一一阐述,比较全面的总结和解释了影响因素意义和特点。最后从网络论坛用户、网络论坛管理者、合作机构和学术研究机构几个角度分析了对网络论坛质量的研究的重要性,阐明了评价网络论坛质量的影响因素的应用价值。     

中国女篮第30届奥运会比赛能力分析

采用文献资料法、录像观察法、数理统计法和比较分析法对第30届伦敦奥运会女篮比赛进行研究,以为中国女篮运动水平的提高提供理论依据.结果表明:中国女篮水平下滑,比赛综合能力排名C级.中国女篮急需有针对性的训练来缩短与世界强队的距离.