A microdevice for the creation of patent, three-dimensional endothelial cell-based microcirculatory networks

Microvascular network formation is a significant and challenging goal in the engineering of large three-dimensional artificial tissue structures. We show here the development of a fully patent, 3D endothelial cell (microvascular) microfluidic network that has a single inlet and outlet, created in only 28 h in a microdevice involving fluid flow equivalent to natural vasculature. Our microdevice features a tailored "multi-rung ladder" network, a stylized mimic of an arterial-to-venous pedicle, designed to also allow for systematic and reproducible cell seeding. Immunofluorescence staining revealed a highly contiguous endothelial monolayer (human umbilical vein endothelial cells) throughout the whole network after 24 h of continuous perfusion. This network persisted for up to 72 h of culture, providing a useful template from which the effects of surface chemistry, fluid flow, and environmental conditions on the development of artificial vascular networks ex vivo may be rapidly and robustly evaluated.     

Microfluidics-based devices: New tools for studying cancer and cancer stem cell migration

Cell movement is highly sensitive to stimuli from the extracellular matrix and media. Receptors on the plasma membrane in cells can activate signal transduction pathways that change the mechanical behavior of a cell by reorganizing motion-related organelles. Cancer cells change their migration mechanisms in response to different environments more robustly than noncancer cells. Therefore, therapeutic approaches to immobilize cancer cells via inhibition of the related signal transduction pathways rely on a better understanding of cell migration mechanisms. In recent years, engineers have been working with biologists to apply microfluidics technology to study cell migration. As opposed to conventional cultures on dishes, microfluidics deals with the manipulation of fluids that are geometrically constrained to a submillimeter scale. Such small scales offer a number of advantages including cost effectiveness, low consumption of reagents, high sensitivity, high spatiotemporal resolution, and laminar flow. Therefore, microfluidics has a potential as a new platform to study cell migration. In this review, we summarized recent progress on the application of microfluidics in cancer and other cell migration researches. These studies have enhanced our understanding of cell migration and cancer invasion as well as their responses to subtle variations in their microenvironment. We hope that this review will serve as an interdisciplinary guidance for both biologists and engineers as they further develop the microfluidic toolbox toward applications in cancer research.     

Foot strike patterns of recreational and sub-elite runners in a long-distance road race

Although the biomechanical properties of the various types of running foot strike (rearfoot, midfoot, and forefoot) have been studied extensively in the laboratory, only a few studies have attempted to quantify the frequency of running foot strike variants among runners in competitive road races. We classified the left and right foot strike patterns of 936 distance runners, most of whom would be considered of recreational or sub-elite ability, at the 10 km point of a half-marathon/marathon road race. We classified 88.9% of runners at the 10 km point as rearfoot strikers, 3.4% as midfoot strikers, 1.8% as forefoot strikers, and 5.9% of runners exhibited discrete foot strike asymmetry. Rearfoot striking was more common among our sample of mostly recreational distance runners than has been previously reported for samples of faster runners. We also compared foot strike patterns of 286 individual marathon runners between the 10 km and 32 km race locations and observed increased frequency of rearfoot striking at 32 km. A large percentage of runners switched from midfoot and forefoot foot strikes at 10 km to rearfoot strikes at 32 km. The frequency of discrete foot strike asymmetry declined from the 10 km to the 32 km location. Among marathon runners, we found no significant relationship between foot strike patterns and race times.     

A Layered Framework for Considering Open Science Practices

The open science movement, although not new to social science broadly, has gained momentum recently within communication science. In response, journals in our field have begun encouraging open science practices, from data and materials sharing to submitting preregistered research reports. However, this momentum has also led to some confusion over what is and is not considered open science and what the value of open sciences practices is. In this editorial we lay out an “onion model” of open science that describes increasing levels of transparency and suggests how open science practices can be understood less as a revolutionary concept but more as a logical extension of some of the historical pillars of scientific norms. Through this model, we provide tangible steps for how scholars may begin thinking about how to introduce open science practices into their current and future empirical efforts.     

On Food,Farming and Land Management: Towards a research agenda to reconnect urban and rural lives

Science education has a key role to play in helping people to develop their understanding of the local and global dimensions of food, farming and land management. Based on a review of the literature on what is known about young people’s (3–19) views towards and learning about these topics, a research agenda is outlined for consideration by the science education research community.     

Co-Rumination Partially Mediates the Relationship Between Social Support and Emotional Exhaustion Among Graduate Students

Graduate students regularly report high levels of stress and burnout. Many of those same students utilize social support networks, which can act as stress buffers. This study evaluated excessive negative talk about issues (co-rumination) and its effects on that social support-to-burnout (emotional exhaustion) relationship and predicted that co-rumination would act as a suppressor variable. Graduate student volunteers (N = 213) reported their levels of social support, co-rumination, and emotional exhaustion. Data indicated that co-rumination did mediate the social support-to-emotional exhaustion relationship on 2 dimensions. This project purports that, although social support is important, the content of socially supportive interactions may also be important when attempting to intervene in stressful situations, especially when those interactions involve co-ruminative messages.     

Proposed Guidelines for Operating Counselor Education and Supervision Training Clinics

The purpose of this article is to justify and present a set of guidelines for the effective and ethical administration of counselor education and supervision training clinics. Responding directly to a call for creating guidelines, the authors address core issues surrounding their development. Benefits for clear and accessible guidelines and risks associated with their absence are covered. Drawing upon existing contributions from the field and related professions, guidelines are proposed along with a plan for their approval and implementation in counselor education training clinics.     

Concept of Care,Caring Expectations,and Caring Frustrations of the Elderly Suffering from Chronic Illness

While it is true that elderly concepts of care and caring expectations have been ascertained in previous literatures, little is known about how the elderly population views caring frustrations—particularly that of the Filipino elderly. This study purports to surface the lebenswelt of healthcare expectations and frustrations based on the repondents’ concept of care. A three-part instrument consisting of robotfoto, picture prompts, and unstructured interviews was used to elicit pertinent data. Through the use of dendrogram, field texts were phenomenologically reduced to emerge conceptual clusters. On the whole, this study yielded interesting conceptualizations which include the Clock of Care, the Clock of Caring Expectations and the Clock of Caring Frustrations. The emerged meanings in this study create a picture of care as a driving force in the progression of care expectations and frustrations. This eidetic background enables healthcare providers to offer care that is both timely and relevant to their client's subjective illness experience.