The effect of extremely high glucose concentrations on 21 routine chemistry and thyroid Abbott assays: interference study

Introduction

Extremely high glucose concentrations have been shown to interfere with creatinine assays especially with Jaffe method in peritoneal dialysate. Because diabetes is the fastest growing chronic disease in the world, laboratories study with varying glucose concentrations. We investigated whether different levels of glucose spiked in serum interfere with 21 routine chemistry and thyroid assays at glucose concentrations between 17-51 mmol/L.

Materials and methods

Baseline (group I) serum pool with glucose concentration of 5.55 (5.44-5.61) mmol/L was prepared from patient sera. Spiking with 20% dextrose solution, sample groups were obtained with glucose concentrations: 17.09, 34.52, and 50.95 mmol/L (group II, III, IV, respectively). Total of 21 biochemistry analytes and thyroid tests were studied on Abbott c8000 and i2000sr with commercial reagents. Bias from baseline value was checked statistically and clinically.

Results

Creatinine increased significantly by 8.74%, 31.66%, 55.31% at groups II, III, IV, respectively with P values of < 0.001. At the median glucose concentration of 50.95 mmol/L, calcium, albumin, chloride and FT4 biased significantly clinically (-0.85%, 1.63%, 0.65%, 7.4% with P values 0.138, 0.214, 0.004, < 0.001, respectively). Remaining assays were free of interference.

Conclusion

Among the numerous biochemical parameters studied, only a few parameters are affected by dramatically increased glucose concentration. The creatinine measurements obtained in human sera with the Jaffe alkaline method at high glucose concentrations should be interpreted with caution. Other tests that were affected with extremely high glucose concentrations were calcium, albumin, chloride and FT4, hence results should be taken into consideration in patients with poor diabetic control.Key words: assay interference, glucose interference, preanalytical phase, creatinine, Jaffe kinetic assay, thyroid function tests     

供应链上下游企业合作研发模式选择--宝钢-大众激光拼焊项目案例分析

企业供应链的发展促使企业合作研发寻求新的模式。分析了上下游大型企业进行合作研发的诱因及其模式选择问题,结合宝钢一上海大众激光拼焊案例,重点讨论了建立股权制合作研发对上下游企业的影响和供应链创新的意义。     

Using the web to encourage student-generated questions in large-format introductory biology classes

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Marital conflict and conduct problems in Children of Twins

The Children-of-Twins design was used to test whether associations between marital conflict frequency and conduct problems can be replicated within the children of discordant twin pairs. A sample of 2,051 children (age 14-39 years) of 1,045 twins was used to estimate the genetic and environmental influences on marital conflict and determine whether genetic or environmental selection processes underlie the observed association between marital conflict and conduct problems. Results indicate that genetic and nonshared environmental factors influence the risk of marital conflict. Furthermore, genetic influences mediated the association between marital conflict frequency and conduct problems. These results highlight the need for quasiexperimental designs in investigations of intergenerational associations.     

The role of gender constancy in early gender development

Kohlberg's (1966) hypothesis that the attainment of gender constancy motivates children to attend to gender norms was reevaluated by examining these links in relation to age. Ninety-four 3- to 7-year-old children were interviewed to assess whether and how constancy mediates age-related changes in gender-related beliefs. As expected, results indicated a general pattern of an increase in stereotype knowledge, the importance and positive evaluation of one's own gender category, and rigidity of beliefs between the ages of 3 and 5. Moreover, the stability phase, rather than full constancy, mediated some of these relations. After age 5, rigidity generally decreased with age, with relations primarily mediated by consistency.     

ISM-CMAP-Combine (ICMC) for hierarchical knowledge scenario in quality engineering education

Quality assurance in engineering education is a multifaceted problem further augmented by the stakeholders of engineering education. Students, parents and employers are rightfully questioning the quality of the curriculum, the instructional delivery, the learning environment, accessibility to learning technologies and equipment, employability as well as the possibilities of lifelong learning and the improvement of knowledge (Karapetrovic 2002 Karapetrovic, S. 2002. Why and how to develop a meaningful quality assurance system in engineering schools. Int. J. Engg. Educ, 18(3): 285–294. [Web of Science ®] [Google Scholar]). The purpose of the study is, therefore, to identify the parameters that influence the quality of an engineering education system and develop an integrated model of hierarchical structuring and a concept map for pre-identified concept elements through a process of iterative-interactive consensus-driven methodology, as advocated by second-generation system design paradigm for evolving knowledge scenario of concepts related to quality engineering education.     

The Intersection of Race and Gender Among Black American Adolescents

This study employed an exploratory sequential mixed-methods design to examine the intersection of race and gender among Black American adolescents, aged 11–19. The quantitative component (n = 344) used survey data to examine gender differences in peer-perpetrated and adult-perpetrated racial discrimination experiences, and no gender differences were evident. Qualitative data (n = 42) probed how males and females interpret discrimination experiences given the intersection of race and gender. Although the majority of participants believed that Black males and females have similar experiences, some believed that Black males face more racial discrimination. However, analyses revealed social disadvantages for Black females given that they report inappropriate comments and unwelcome hair touching and limited opportunities for interracial dating compared with Black males.     

Adolescents’ Friendships,Academic Achievement,and Risk Behaviors: Same-Behavior and Cross-Behavior Selection and Influence Processes

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