一种实验教学模式的探索与实践

结合教学实践总结出一种突出学生主体地位的实验教学模式.实践证明该模式对于培养学生掌握科学实验方法和技能,提高学生科学素质、动手能力与思维能力是行之有效的.     

E-凸集上的Ky Fan''''s 不等式及其应用

证明了B凸集上的Ky Fan’s不等式，并运用B凸集上的Ky Fan’s不等式，得到了E-凸集上的不动点定理.     

《工程材料及成形技术》教学改革的行与思

在对“工程材料及成形技术”及其实验课的课程教学改革实践中,增加了在线课堂学习,丰富了实验课的教学内容,据此对课堂教学的形式和考评机制进行了改革和实践探索,并对教改效果进行了反思。实践证明,教改在提升学生自主学习能力的同时,也有一些问题值得我们去反思、探索与改进。     

论戴望舒诗歌创作中的悲剧意识

戴望舒作为20世纪30年代现代派诗坛的首领,他强调新诗“应该有新的情绪“.也就是在这种“内在情绪“的牵引下,戴望舒的九十余首诗篇中流露出浓重的悲剧意识.本文拟从戴望舒悲剧意识的缘起、文本呈现及其得失等三个方面作一粗浅的分析.     

“三美论”观照下的《再别康桥》两译本评析

以许渊冲的“三美论”为导向, 对Mr . Ediard Connynkam和丁祖馨、拉菲尔的《再别康桥》两译本进行评析, 旨在洞察不同的译者对原诗不同的解读, 考察译者的翻译风格。     

陕北民歌中的民俗文化

本文对陕北民歌做了简单的归类介绍 ,揭示了民歌与民俗文化相互渗透 ,相互发展的关系     

师范学生的素质教育重在培养动手能力

针对当前师范生的现状 ,指出了师范生的素质教育与动手能力培养的内在关系 ,提出了几点建议     

生存之惑:世纪之交的中国电影类杂志发展趋势研究

作为中国电影历史的实时记录者与电影文化的有力传播者,我国的电影类期刊在世纪之交与我国电影产业一样经历了好莱坞等外国电影业的冲击。本文对我国电影类杂志在20世纪末、21世纪初的发展趋势进行仔细解读,以期为我国电影杂志发展史的研究补上不可或缺的一环,并进一步明确我国电影期刊的发展方向。     

小学生自我概念训练的实验研究

通过Piers-Harris儿童自我概念量表筛选出三至五年级低自我概念的学生,实验组接受认知训练,控制组不接受训练。认知训练以后,三个年级的实验组的自我概念水平提高的幅度均比控制组的幅度要大,经统计检验,差异都非常显著。这表明:(1)认知训练可提高学生的自我概念水平;(2)小学三到五年级自我概念水平的训练效果不存在年龄差异;(3)小学三到五年级自我概念的训练效果不存在性别差异。     

Investigation on apoptosis of neuronal cells induced by Amyloid beta-Protein

Objective: To construct a PC12 cell strain with neuronal differentiation, and observe the apoptosis and pro-liferation activity effects induced these cells by Amyloid beta-Protein (Aβ-43). Methods: 1) PC12 cells in logarithmicgrowth phase were subcultured for 24 h. After the culture fluid was changed, the cells were treated with Rat-β-NGF andcultured for 9 days. 2) Neuronal differentiation of PC12 cells in logarithmic growth phase were divided into four groups:control group (0), experimental group (1), experimental group (2) and experimental group (3). The concentrations of Aβ inthe four groups were 0 μmol/L, 1.25 μ mol/L, 2.5 μ mol/L and 5 μmol/L, respectively. The cells were harvested at 24, 48 and72 h later and stained with AnnexinV-FITC/PI after centrifugation and washing. Then flow cytometry was conducted toexamine the apoptosis percentage. 3) NGF-induced PC12 cells were selected and Aβ with different concentrations wasadded. The final concentrations of Aβ were 0 μmol/L, 1.25 μmol/L, 2.5 μmol/L and 5 μ mol/L, respectively. After the cellswere incubated in an atmosphere of 5% CO2 at 37 ℃ in an incubator for 72 h, the OD values were examined. Results: 1)Neuronal differentiated PC 12 cell lines were successfully established. 2) Flow cytometric examination indicated that Aβ(1.25, 2.5, and 5.0 μmol/L) could effectively induce apoptosis of neuronal-differented cells at the 24 h, 48 h and 72 h timepoints. 3) Aβ (0-5.00 μ mol/L) had no obvious effect on proliferation or restraining of the neuronal differentiation of thePC 12 cells after a 72 h interacting process. Conclusion: This investigation revealed successful neuronal differentiation of thePC12 cell strain. The induction of apoptosis of the neurocytes by various concentrations of Aβ was observed and the in-fluence of Aβ on induced proliferation of PC 12 cells by Rat-β-NGF was revealed. This study -05 provide basis for futureresearch on the molecular cure of AD and interdiction of AD evolution.