Preliminary studies on the antitubercular activity and the mechanism of action of the water extract of garlic (Allium sativum) and its two partially purified proteins (Garlic defensins?)

Water extract of garlic (Allium sativum) inhibited the growth ofMycobacterium tuberculosis H₃₇R_v andM. tuberculosis TRC-C1193 susceptible and resistant to isoniazid respectively. The minimum inhibitory concentration (MIC) was slightly above 80 but less than 160 μg/ml and slightly above 100 but less than 200 μg/ml for the susceptible and resistant strains respectively. Gel filtration in Sephadex G-100 columns showed that two protein fractions (43 & 38 kD) possessed antitubercular activity with much lower MICs of 20–40 μg/ml and 30–60 μg/ml for susceptible strain. Water extract, when added to actively growingM. tuberculosis in their mid log phase prevented their further growth. The water extract of garlic inhibited the incorporation of¹⁴C glycine into whole cells by 81% in 6 hrs. indicating that the primary mechanism of action is by inhibition of protein synthesis.     

Effect of varying durations of pyramid exposure — an indication towards a possibility of overexposure

Miniature replicas modeled after the Great Pyramid of Giza are believed to concentrate geoelectromagnetic energy within their cavities and hence act as antistressors in humans and animals. Although there are not many reports of adverse effects of ‘overexposure’ in the pyramid, subjects have claimed to feel uneasy after certain duration of staying in the pyramid. The present study was aimed to analyze the effects of prolonged pyramid exposure on plasma cortisol level, markers of oxidative damage and antioxidant defense in erythrocytes of adult female Wistar rats. Rats were divided into three groups, normal controls (NC, n=6) that were maintained under standard laboratory conditions in their home cages, pyramid exposed group-2 (PE-2, n=6) & pyramid exposed group-4 (PE-4, n=6) where the rats were housed under the pyramid for 6 hours/day for 2 weeks and 4 weeks respectively. Plasma cortisol and erythrocyte TBARS levels were significantly lower in both PE-2 and PE-4 rats and erythrocyte GSH levels and GSH-Px activity were significantly higher in them as compared to the NC rats. There was no significant difference in the results for these parameters between the PE-2 and PE-4 rats except for erythrocyte GSH-Px activity which was significantly more in the PE-2 rats than in the PE-4 rats. Although these results don’t confirm any adverse effects of prolonged exposure in pyramids, they indicate a possibility of such adverse effects.     

Cancer genes: The molecular militants

‘What actually seems to have happened when a cancer appears in an organism is that the biological behavior of some of the cells which make up that organism have changed. Whereas these cells had hitherto behaved in a manner consistent with the life of the organism, obeying the ‘rules’ of growth and differentiation, they quite suddenly cease to respond to such restraints and set off on a ‘lawless’ course of increased division, invasion of adjacent normal tissues, and even migration (metastasis) via the blood stream and lymph channels to other parts of the body.’ R J C Harris inCancer, 1962.     

Introduction: the “vectors” of Chinese history

This issue contains essays by the important Japanese sinologist and intellectual historian, Mizoguchi Yūzō (1932–2010). Born in Nagoya, he studied in the literature department in Tokyo University and then went to graduate school in Nagoya University. He was a student of Iriya Yoshitaka, the famous sinologist. Mizoguchi has taught at Saitama University, Hitotsubashi University and Tokyo University. He was also a Distinguished Professor at Tokyo University. Mizoguchi Yūzō left a number of important works throughout his life. The selection is unable to provide a complete picture of Mizoguchi’s ideas about the principle of Chinese history, but it provides an entry point for English readers to understand Mizoguchi’s work. In this introduction, we attempt to bring out certain key ideas of Mizoguchi’s writings and discuss his historical and theoretical significance.     

A standalone perfusion platform for drug testing and target validation in micro-vessel networks

Studying the effects of pharmacological agents on human endothelium includes the routine use of cell monolayers cultivated in multi-well plates. This configuration fails to recapitulate the complex architecture of vascular networks in vivo and does not capture the relationship between shear stress (i.e. flow) experienced by the cells and dose of the applied pharmacological agents. Microfluidic platforms have been applied extensively to create vascular systems in vitro; however, they rely on bulky external hardware to operate, which hinders the wide application of microfluidic chips by non-microfluidic experts. Here, we have developed a standalone perfusion platform where multiple devices were perfused at a time with a single miniaturized peristaltic pump. Using the platform, multiple micro-vessel networks, that contained three levels of branching structures, were created by culturing endothelial cells within circular micro-channel networks mimicking the geometrical configuration of natural blood vessels. To demonstrate the feasibility of our platform for drug testing and validation assays, a drug induced nitric oxide assay was performed on the engineered micro-vessel network using a panel of vaso-active drugs (acetylcholine, phenylephrine, atorvastatin, and sildenafil), showing both flow and drug dose dependent responses. The interactive effects between flow and drug dose for sildenafil could not be captured by a simple straight rectangular channel coated with endothelial cells, but it was captured in a more physiological branching circular network. A monocyte adhesion assay was also demonstrated with and without stimulation by an inflammatory cytokine, tumor necrosis factor-α.     

Effects of Simulated Interventions to Improve School Entry Academic Skills on Socioeconomic Inequalities in Educational Achievement

Randomized controlled trial evidence shows that interventions before age 5 can improve skills necessary for educational success; the effect of these interventions on socioeconomic inequalities is unknown. Using trial effect estimates, and marginal structural models with data from the Avon Longitudinal Study of Parents and Children (n = 11,764, imputed), simulated effects of plausible interventions to improve school entry academic skills on socioeconomic inequality in educational achievement at age 16 were examined. Progressive universal interventions (i.e., more intense intervention for those with greater need) to improve school entry academic skills could raise population levels of educational achievement by 5% and reduce absolute socioeconomic inequality in poor educational achievement by 15%.