One step antibody-mediated isolation and patterning of multiple cell types in microfluidic devices

Cell-cell interactions play a key role in regeneration, differentiation, and basic tissue function taking place under physiological shear forces. However, current solutions to mimic such interactions by micro-patterning cells within microfluidic devices have low resolution, high fabrication complexity, and are limited to one or two cell types. Here, we present a microfluidic platform capable of laminar patterning of any biotin-labeled peptide using streptavidin-based surface chemistry. The design permits the generation of arbitrary cell patterns from heterogeneous mixtures in microfluidic devices. We demonstrate the robust co-patterning of α-CD24, α-ASGPR-1, and α-Tie2 antibodies for rapid isolation and co-patterning of mixtures of hepatocytes and endothelial cells. In addition to one-step isolation and patterning, our design permits step-wise patterning of multiple cell types and empty spaces to create complex cellular geometries in vitro. In conclusion, we developed a microfluidic device that permits the generation of perfusable tissue-like patterns in microfluidic devices by directly injecting complex cell mixtures such as differentiated stem cells or tissue digests with minimal sample preparation.     

Arts education partnerships: sources of harmony and dissonance with cultural institutions’ collaborative efforts

ABSTRACT

Due to budget constraints, schools in the United States have increasingly turned to community arts organizations for support. School-community arts partnership stakeholders collaborate because of shared missions to provide students with valuable arts learning experiences. Investigations of these initiatives indicate that these partnerships improve arts learning opportunities and increase public support and resources for arts education. However, not much is known about the experiences and perspectives of the arts organizations that participate in these partnerships. Coordinating collective efforts with a multitude of institutions and interests poses challenges. In this study, we examine survey data collected from arts organization administrators who participated in a large-scale school-community arts partnership initiative. We find that these organizations are generally positive about their impacts on students’ educational outcomes, but there is substantial variation in these views. We also find that organizations differ in their levels of support for these collective efforts. Sources of this variation appear to be attributable to organizations’ preexisting resources and extent to which they are established. While this difference in levels of support is potentially inevitable, we find evidence that the operations handled by the “backbone” organization, i.e. the initiative’s facilitators and overseers, can significantly influence organizations’ levels of support for these efforts. Organizations are more likely to support these collaborative efforts when they believe the backbone organization ensures transparency with initiative operations, provides regular, effective communication, and effectively resolves competing priorities.     

The Effects of Self‐ and Peer‐Monitoring in Social Studies Performance of Students with Learning Disabilities and Low Achieving Students

This study employed group randomized trials to investigate the effects of self‐ and peer‐monitoring on the academic vocabulary and content knowledge of students with learning disabilities and low achieving students in social studies. Fourth grade students were randomly assigned to either treatment or control groups on a class level. Results indicated that there was a significant interaction effect between the intervention and students’ pretest scores on academic vocabulary acquisition; the intervention was more effective for students with lower scores in the pretest than those with higher scores. In addition, there was a significant intervention effect on content knowledge acquisition; the effect size of the growth of the treatment group on the content knowledge test was significantly larger than that of the comparison group. Teacher interviews also revealed that teachers viewed the intervention positively. Implications for practice and suggestions for future research are discussed.     

Cross-Cultural Adaptation and Psychometric Properties of the Portuguese Version of the Life Skills Scale for Sport

ABSTRACT

This research adapted the Life Skills Scale for Sport (LSSS) into Portuguese and provided evidence for its construct validity. Study 1 included four translators and five academics who developed a Portuguese version of the LSSS (P-LSSS). During this study, evidence for the content and substantive aspects of construct validity was provided using an expert panel and 25 sports participants. Study 2 included 413 participants that completed the P-LSSS. Within this study, evidence for the structural aspect of construct validity was provided via factor analyses. Study 3 included 134 participants who completed the P-LSSS and a measure of motivation. This study provided evidence for the external aspect of construct validity, with results showing more self-determined motivation had positive relationships with participant’s life skills development. Overall, our findings provided evidence for the construct validity of P-LSSS scores. Researchers and practitioners can use the P-LSSS to assess life skills development within sports participants.     

Fantasy,Pragmatism and Journalistic Socialisation: UK Journalism Students’ Aspirations and Motivations

ABSTRACT

Despite the sustained growth in journalism as a choice of degree path for young people, our understanding of students’ aspirations and motivations remains relatively underdeveloped. At the same time, journalism careers appear increasingly uncertain, as the industry responds to digitalisation and convergence. In this mixed-methods study – employing 35 interviews and a survey of 837 UK journalism students – we ask what areas of journalism do students aspire towards, how do they feel about their future career prospects, and what is motivating them to study journalism in the first place? We find that intrinsic motivations (calling and talent, dynamic job) prevail over public service ones, with students drawn to soft news beats over hard news. Aspirations are also strikingly gendered, opening up questions of journalism education in this process. We also find that while students articulate an aspirational career in respected media outlets, they are pragmatic about their immediate career prospects. Here, journalism education appears to play a significant role in socialising students towards careers beyond journalism. Findings are discussed in the light of ongoing debates around journalistic socialisation and the future of journalism.