Glial cells: The other cells of the nervous system

Carl Ludwig Schleich (1859–1922) was an anaesthetist and a surgeon who for the first time emphasized the role of neuroglia in brain function. In an era that was dominated by the idea that neurons alone were functional units of the nervous system and that glial cells were a mere glue holding neurons in place, Schleich insisted that glia had a prominent functional role to play. He suggested that interactions between neurons and glia were so strong that it is necessary to consider brain as a neuron-glia system whose performance is dependent on both the cell types. Today Carl Ludwig Schleich is considered the forgotten ancestor to a new breed of neurobiologists, the gliologists, who specialize in working on these neglected cells of the nervous system. Though outnumbering the neurons and occupying almost half the volume of the brain, glial cells have been given little importance in textbooks of neurobiology and are very often cursorily mentioned in discussions related to brain function. Glial cells deserve a better deal, for over the years a lot of evidence has accumulated to prove that they perform a wide spectrum of functions. This is the first of a series of articles that aims to update students on what is known about glia today. It provides an overview of the various types of glia and their origins. The following articles will deal with each of the glial sub-types and their functions.     

Antioxidant status in neonatal jaundice before and after phototherapy

Phototherapy has been related to increased oxidative stress and lipid peroxidation. In the present study, thirty full term jaundiced neonates with appropriate weight were analyzed before and after completion of phototherapy for malondialdehyde (MDA), reduced glutathione (GSH), total thiols, vitamin C and superoxide dismutase (SOD) levels in hemolysate and albumin levels in plasma. These parameters were analyzed in cord blood samples of 20 healthy neonates as control. It was observed that levels of MDA were elevated significantly (p<0.001) in patients as compared to controls and that the levels increased significantly after phototherapy (p<0.001). Levels of SOD were also found to be increased significantly as compared to controls and the levels rose after phototherapy (p<0.001). On the other hand, the levels of non-enzymatic antioxidants such as GSH, total thiols and vitamin C were significantly low (p<0.001) as compared to controls and the levels decreased significantly after phototherapy (p<0.001). The plasma albumin levels also were found to be decreased significantly after phototherapy (p<0.01). Therefore, phototherapy increases oxidative stress and should be used with care.     

The flexible university: higher education and the global production of migrant labor

This article demonstrates how neoliberal higher education has come to play a distinct role in the global market for migrant labor, where a growing number of developing nations educate its citizens for overseas work in order to maximize future monetary remittances. Located in the Philippines, this study shows how local colleges and universities attempt to impose an ideal notion of flexibility, quickly shifting academic manpower and resources to programs that would produce the ‘right’ types of workers to address foreign labor demands. Based on qualitative interviews with Filipino college educators and students, the article then discusses how such ‘flexible’ strategies undermine the job security of college faculty and lead to the constant restructuring of physical space within university campuses. Such changes negatively affect both students and teachers.     

Hypolipidemic effect of water extract ofFicus bengalensis in alloxan induced diabetes mellitus in rabbits

Hypolipidemic effect of the water extract of the bark ofFicus bengalensis was investigated in alloxan induced diabetes mellitus in rabbits. Treatment for one month (50 mg/kg body weight/day) brought down the level of total serum cholesterol (TC) in subdiabetic and diabetic rabbits (five in each group) from 82±11 mg% and 118±10.6 mg% to 42.7±3.1 mg% and 51.7±4.7 mg% respectively. Low density lipoprotein cholesterol and very low density lipoprotein cholesterol values came down from 34±10 mg% and 95±24 mg% to 16±3 mg% and 29±4 mg% in subdiabetic and diabetic rabbits. Triacylglycerol level before treatment was 121±21.6 mg% and 416±70 mg% in subdiabetic and diabetic rabbits. Treatment brought it down to 45±5 mg% and 81±27.5 mg%. Glycosylated hemoglobin was brought down from 2.1±0.3% to 1.5±0.1% in subdiabetic group and from 4.28±0.5% to 2±0.3% in diabetic group, indicating that treatment with water extract effectively controlled blood sugar. After treatment with water extract serum lipid values were nearly equal to those in healthy controls (normal) in subdiabetic rabbits, while in diabetic group, the values were only slightly above those of normal.     

Immunization with <Emphasis Type="Italic">Wuchereria bancrofti</Emphasis> Glutathione-S-transferase Elicits a Mixed Th1/Th2 Type of Protective Immune Response Against Filarial Infection in Mastomys

Lymphatic filariasis is a mosquito borne parasitic infection and can severely affect the normal working ability of an individual. Currently there is no vaccine available to prevent this infection and the development of a potential vaccine could effectively support the on-going mass drug administration program by World Health Organization (WHO). Filarial parasites have complex mechanisms to modulate the host immune responses against them. The glutathione-S-transferases (GST) are the important enzymes effectively involved to counteract the oxidative free radicals produced by the host. In the present study, we have shown that the mastomys which are fully permissible rodents for Brugia malayi when immunized with Wuchereria bancrofti recombinant GST (rWbGST) could induce 65.5 % in situ cytotoxicity against B. malayi infective (L₃) larvae. There was a balanced Th1/Th2 immune response in the vaccinated animals, characterized by higher levels of WbGST-specific IgG1 and IgG2a antibodies and pronounced IFN-γ, IL-10 and IL-4 cytokines production by the spleen cells.     

Lectin in agglutination and its role in detection of sugars on the surface of RBC of normal and cancer patients

In the present studies the property of Lectins as agglutinin and to bind sugars in the blood on normal and cancer cervix patients has been studied. Out of 36 lectins extracted from the seeds of various plants, 25 were non-specific haemagglutinins and 11 were non-reactive with the RBC of normal persons. The titre value greatly varied from 1∶4 to 1∶4096. The titre value of RBC of patients of carcinoma cervix (Stage III) was compared with the normal person’s RBC. Out of 25 non-specific haemagglutinins 16 lectins did not show any difference in titre value. Nine lectins agglutinated RBC of cancer patient at a higher dilution than the normal. On the contrary, 3 lectins agglutinated the cancerous patient’s RBC at a higher concentration than the normal (Control). Sugar specificity of 25 non-specific haemagglutinins was tested with 13 sugars separately. Haemagglutination Inhibition (HI) reaction showed marked difference in the RBC of normal and in carcinoma cervix patient. Four marked reactions were noticed. RBC of normal (N) and carcinoma patient (C) with positive HI reaction (C₊/N₊), with negative HI reaction (C_?/N_?); with C positive and N negative HI reaction (C₊/N_?) and with C negative and N positive HI reaction (C_?/N₊). Three lectins were specific to sugar moieties.Vicia faba JV II showed dissimilarity with fructose and glucose moiety and also indicated that cellulose is common in both RBC of normal and cancer cervix patient.Triticum vulgare lectin showed the difference in cellulose moiety.Linum usitatissimum lectin showed the difference in lactose moiety and indicated that melibiose is present in both. It was also observed that mannose gave positive HI reaction with lectins ofR. communis, L. esculentus, P. sativum, P. sativum Pusa 10,P. sativum R?1,D. lablab, A. indica, H. vulgare andZ. mays in the blood of cancer cervix patient suggesting that mannose perhaps is present in the RBC while it may not be present in the normal person’s RBC. Raffinose also gave the positive HI reaction with cancer patient’s blood and not with the normal person’s blood.     

Non enzymatic glycosylation of IgG and their urinary excretion in patients with diabetic nephropathy

Diabetic nephropathy is a major cause of end stage renal disease. Increased excretion of albumin has widely been recognized as an early manifestation of diabetic nephropathy particularly in subjects with diabetes mellitus. However, certain other proteins besides albumin may be excreted in high amount during early phase of diabetic nephropathy. The serum and urinary IgG, Glycosylated hemoglobin, fructosamine and glycosylated IgG were evaluated in the present study. Thirty-two patients of Type 2 Diabetes without any complications, thirty-one patients of Type 2 Diabetes with nephropathy, twenty-six patients of non-diabetic nephropathy and forty normal healthy individuals were enrolled in this study. Subjects were grouped based on their serum creatinine level. Serum IgG, glycosylation of IgG and urinary IgG excretion were increased significantly in diabetic patients compared to healthy controls, which were further increased significantly in chronic renal failure patients with respect to the clinical stage of nephropathy. A positive correlation was observed between glycosylation of IgG and IgG excretion (R²=0.5995, 0.7114 respectively) in diabetic patients without any complications and diabetic nephropathy patients only, suggesting a significant role of IgG glycosylation in the vascular clearances of IgG during diabetic nephropathy.     

Clinical correlation of alteration of endogenous antioxidant-uric acid level in major depressive disorder

Derangement of antioxidant levels in major depressive disorder had been correlated with oxidative damage. The effect of Selective Serotonin Re-Uptake Inhibitors on endogenous antioxidant uric acid levels in major depressive disorder has never been examined. This was a prospective; open labeled, parallel, 12 weeks study, in which serum uric acid levels and Hamilton Rating Scale for Depression score were estimated in age and sex matched thirty-six healthy and forty major depressive disorder subjects before and after fluoxetine and citalopram treatment. Significant decrease in serum uric acid (P<0.0001) was observed in newly diagnosed major depressive disorder subjects when compared to healthy subjects. The trend was reversed after 6 weeks more significantly after 12 weeks of treatment with improvement in Hamilton Rating Scale for Depression score. Also, Significant and negative correlation was found between Hamilton Rating Scale for Depression score and serum uric acid level (r= −0.864, P<0.001) after 12 weeks of treatment. Treatment with fluoxetine or citalopram reverses endogenous antioxidants like uric acid and improves Hamilton Rating Scale for Depression score in major depressive disorder.     

Mobilizing a Narrative of Generosity: Patient Experiences on an Urban Mobile Health Clinic

Mobile health clinics address the health needs of underserved populations by bringing healthcare to patients’ communities. Mobile health clinics find unique ways to treat patients and empower them to take control of their health. In this article, we examine how patients who use an urban mobile health clinic narrate their experiences on the clinic and what they see as the impact of the clinic on the community. A narrative of generosity emerged from the interviews, where patients believe the mobile health clinic creates a sense of welcome, encourages patients to become active participants in their health, and fosters a “pay it forward” attitude among community members.