Core-shell hydrogel beads with extracellular matrix for tumor spheroid formation

Creating multicellular tumor spheroids is critical for characterizing anticancer treatments since they may provide a better model of the tumor than conventional monolayer culture. Moreover, tumor cell interaction with the extracellular matrix can determine cell organization and behavior. In this work, a microfluidic system was used to form cell-laden core-shell beads which incorporate elements of the extracellular matrix and support the formation of multicellular spheroids. The bead core (comprising a mixture of alginate, collagen, and reconstituted basement membrane, with gelation by temperature control) and shell (comprising alginate hydrogel, with gelation by ionic crosslinking) were simultaneously formed through flow focusing using a cooled flow path into the microfluidic chip. During droplet gelation, the alginate acts as a fast-gelling shell which aids in preventing droplet coalescence and in maintaining spherical droplet geometry during the slower gelation of the collagen and reconstituted basement membrane components as the beads warm up. After droplet gelation, the encapsulated MCF-7 cells proliferated to form uniform spheroids when the beads contained all three components: alginate, collagen, and reconstituted basement membrane. The dose-dependent response of the MCF-7 cell tumor spheroids to two anticancer drugs, docetaxel and tamoxifen, was compared to conventional monolayer culture.     

Examining the Rule of Thumb of Not Using Multilevel Modeling: The “Design Effect Smaller Than Two” Rule

Educational researchers commonly use the rule of thumb of “design effect smaller than 2” as the justification of not accounting for the multilevel or clustered structure in their data. The rule, however, has not yet been systematically studied in previous research. In the present study, we generated data from three different models (which differ in the location of the clustering effect). With a 3 (design effect) × 5 (cluster size) × 4 (number of clusters) Monte Carlo simulation study we found that the rule should not be applied when researchers: (a) are interested in the effects of higher-level predictors, or (b) have a cluster size less than 10. Implications of the findings and limitations of the study are discussed.     

Integrating Creative Thinking Skills Pedagogies into a Higher Education Visual Arts Course

Although there has been increasing international emphasis on creativity in education, many creativity training programmes have focused on enhancing students' creative thinking skills with few studies on how these skills can be integrated into the teaching of subject disciplines. As a member of a Community of Practice project that ran from spring 2021 to summer 2022 at my university, I worked with ten university teachers from multiple disciplines to develop and implement instructional strategies to foster students' creative thinking skills. The paper documented the development, implementation and evaluation of creative thinking skills teaching strategies for a higher education course in visual arts. Both the development of the teaching strategies and the measurement of the impact on student learning have undergone vigorous research procedures and made reference to the existing literature. The effectiveness of the activities was assessed using multiple methods including the Torrance Test of Creative Thinking, a self-report inventory, and a focus group interview. The results indicated the new learning activities enhanced students' creative thinking skills. They also showed that creativity can be developed through teaching while revealing that playfulness, freedom and structure, group interactions, and problem-solving activities are beneficial for the development of creative thinking skills. Readers may better understand the different ways in which creative thinking skills instruction materials can be developed and incorporated into teaching of visual arts by making reference to the strategies suggested and the process of development in the paper.     

The Perfect Marriage? – Language and Art Criticism in the Hong Kong Public Examination Context

Art education in Hong Kong has undergone various changes in response to educational reform. In art assessment, a major change in the Hong Kong New Senior Secondary (NSS) Curriculum is the inclusion of art criticism as a compulsory component of the new public examination. Assessing students’ abilities to interpret art in an art criticism public examination context is a critical issue in Hong Kong because the new senior secondary curriculum and assessment has brought attention to the role of written language in the art examination paper. This means the examination assesses not only students’ abilities to interpret art, but also their language abilities required to respond to art in written form. Since this new mode of assessment of art criticism has been published a number of issues have appeared. Recent studies show that teachers and students perceive this development negatively and they believe that the written format will assess students’ written language abilities rather than their critical abilities. These findings challenge the justification of the new art assessment policy and raise questions about the role of written language in responding to art. This article aims to raise the issue of the marriage between language and art criticism in the Hong Kong public examination context. It argues and examines the relationship of language to art interpretation, reasoning in the assessment, and issues in the public art criticism examination context. The issues addressed in this article provide opportunities for researchers and policy makers to reconsider and refine the new form of examination.     

Novice teacher vertical professional development? Exploring teachers’ and coaches’ beliefs throughout a two-year induction program

Learning Environments Research - The pedagogical beliefs of novice teachers and their coaches throughout a two-year induction program and how those beliefs impact their induction experiences were...     

Teachers’ perception of mobile edutainment for special needs learners: the Malaysian case

Study of Malaysian adoption of mobile learning (m-learning) is still in the early stages. However, there are numerous researchers in the country exploring the potential and application of m-learning in the Malaysian education system, including special education. A key question is whether teachers are prepared to incorporate mobile technology as part of their teaching tools or do teachers perceive mobile technology only as personal communication gadgets. This paper investigates teachers' perception of m-learning applications in special education classes. Interviews were conducted to collect related information on teachers' perceptions of m-learning in the selected schools. The teachers expressed that there are limited resources to cater to the attention of special needs learners and they believed edutainment technologies such as augmented reality, game-based educational software and animation projects could be the answer. The benefits and challenges of implementing blended learning for special education are also discussed. The paper concludes by summarising the current environment of m-learning in Malaysian special needs education, together with recommendations for future use of the technology.     

Homophobia in medical students of the University of Hong Kong

Homosexuality is now accepted as a normal variant of human sexuality, but homophobia among healthcare professionals is well documented. Establishment of trustful doctor–patient relationships is impossible in the presence of homophobia. We were interested to examine the extent of homophobia among medical students, the future doctors. This article aims to study attitudes of medical students of the University of Hong Kong towards homosexuality and to identify significant factors affecting their attitudes. A cross‐sectional study was performed on students of the University of Hong Kong. Attitudes towards homosexuality were compared between medical and non‐medical students. More than 25% of the medical students agreed with the statement ‘homosexuality is a psychological disorder that requires therapy’. More than 15% would avoid any physical contact with homosexuals to minimise the chance of contracting diseases, especially AIDS. Comparison between the medical students and non‐medical students showed that the attitudes of the medical students were significantly more homophobic. Factors explaining the difference included the importance of religion in the formation of moral values, gender, experience of working closely with homosexual friends, sexual orientation, experience of the heterosexual sexual act, acquaintance of homosexual friends, and experience of homosexual love relationships. In conclusion, there appeared to be a certain level of homophobia among the medical students. This could possibly be explained by their lack of experience of working closely with homosexual friends. Relevant curriculum modification and development are urgently needed, to provide ongoing opportunities for students to realise their own values that could unconsciously affect their clinical judgements.     

A Two-Stage Approach to Synthesizing Covariance Matrices in Meta-Analytic Structural Equation Modeling

A great obstacle for wider use of structural equation modeling (SEM) has been the difficulty in handling categorical variables. Two data sets with known structure between 2 related binary outcomes and 4 independent binary variables were generated. Four SEM strategies and resulting apparent validity were tested: robust maximum likelihood (ML), tetrachoric correlation matrix input followed by SEM ML analysis, SEM ML estimation for the sum of squares and cross-products (SSCP) matrix input obtained by the log-linear model that treated all variables as dependent, and asymptotic distribution-free (ADF) SEM estimation. SEM based on the SSCP matrix obtained by the log-linear model and SEM using robust ML estimation correctly identified the structural relation between the variables. SEM using ADF added an extra parameter. SEM based on tetrachoric correlation input did not specify the data generating process correctly. Apparent validity was similar for all models presented. Data transformation used in log-linear modeling can serve as an input for SEM.     

Multivariate Meta-Analysis as Structural Equation Models

Multivariate meta-analysis has become increasingly popular in the educational, social, and medical sciences. It is because the outcome measures in a meta-analysis can involve more than one effect size. This article proposes 2 mathematically equivalent models to implement multivariate meta-analysis in structural equation modeling (SEM). Specifically, this article shows how multivariate fixed-, random- and mixed-effects meta-analyses can be formulated as structural equation models. metaSEM (a free R package based on OpenMx) and Mplus are used to implement the proposed procedures. A real data set is used to illustrate the procedures. Formulating multivariate meta-analysis as structural equation models provides many new research opportunities for methodological development in both meta-analysis and SEM. Issues related to and extensions on the SEM-based meta-analysis are discussed.